Browsing by Author "Sudip Mukherjee"

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Advancement of Nanomaterials- and Biomaterials-Based Technologies for Wound Healing and Tissue Regenerative Applications
(American Chemical Society, 2025) Durba Banerjee; Kalyan Vydiam; Vangala Venugopal; Sudip Mukherjee
Patients and healthcare systems face significant social and financial challenges due to the increasing number of individuals with chronic external and internal wounds that fail to heal. The complexity of the healing process remains a serious health concern, despite the effectiveness of conventional wound dressings in promoting healing. Recent advancements in materials science and fabrication techniques have led to the development of innovative dressings that enhance wound healing. To further expedite the healing process, novel approaches such as nanoparticles, 3D-printed wound dressings, and biomolecule-infused dressings have emerged, along with cell-based methods. Additionally, gene therapy technologies are being harnessed to generate stem cell derivatives that are more functional, selective, and responsive than their natural counterparts. This review highlights the significant potential of biomaterials, nanoparticles, 3D bioprinting, and gene- and cell-based therapies in wound healing. However, it also underscores the necessity for further research to address the existing challenges and integrate these strategies into standard clinical practice. © 2025 American Chemical Society.
Advances in Nanomedicine for Cancer Theranostics
(Institute of Physics, 2025) Sudip Mukherjee; A. K. Rengan; Chitta Ranjan Patra
Cancer is one of the foremost reasons for global death. According to estimates, around 19.3 million instances of cancer and over 10 million fatalities were documented in the year 2020, making it one of the leading causes of death across the globe. There are still restrictions due to the absence of effective early detection and inadequate conventional therapy, which has led to poor prognosis and survival rates. This is the case despite the fact that there have been breakthroughs in diagnosis and treatment. The science of nanomedicine has achieved considerable advancements in the realm of cancer theranostics. These advancements offer a number of distinct advantages, including tumor-targeting through the increased permeability and retention effect, biocompatibility, and small size. In light of the above, the purpose of the Focus Issue on ‘Advances in Nanomedicine for Cancer Theranostics’, was introduced to highlight new research on nanomedicine-based approaches to cancer treatment. These techniques include drug and gene delivery, bioimaging, biomarker identification, diagnosis, immunotherapy, biosensors, and other precision oncology strategies. For this Focus Issue, we invited front-line researchers and authors who contributed the original research papers and topical review articles. This editorial summarizes the published articles of this collection which includes eighteen research articles and eleven review articles. © 2025 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved.
Correlation between changeover from weak antilocalization (WAL) to weak localization (WL) and positive to negative magnetoresistance in S-doped Bi1.5Sb0.5Te1.3Se1.7
(American Institute of Physics Inc., 2022) Mahima Singh; Labanya Ghosh; Vinod K. Gangwar; Yogendra Kumar; Debarati Pal; P. Shahi; Shiv Kumar; Sudip Mukherjee; K. Shimada; Sandip Chatterjee
The magneto-transport and angle-resolved photoelectron spectroscopy (ARPES) of the S-doped Bi1.5Sb0.5Te1.3Se1.7 system have been investigated. Both the positive magnetoresistance (pMR) and negative magnetoresistance (nMR) under a perpendicular magnetic field as well as a changeover from weak antilocalization (WAL) to weak localization (WL) are observed. The interplay between pMR and nMR is elucidated in terms of the dephasing and spin-orbit scattering time scales. The topological surface state bands have been explored using ARPES. © 2022 Author(s).
Corrigendum to “Engineered cell-laden hemostatic hydrogels for all-round rapid repair of diabetic wounds” [Chem. Eng. J. volume 524 (2025) 169544] (Chemical Engineering Journal (2025) 524, (S1385894725103872), (10.1016/j.cej.2025.169544))
(Elsevier B.V., 2025) Malay Nayak; Durba Banerjee; Devyani Yenurkar; Snehasish Mandal; Lipi Pradhan; Arnab Sarkar; Sudip Mukherjee
The authors regret the use of incorrect schematic diagram in Fig. 4A and its corresponding figure caption. The updated figure (Fig. 4A) and its caption is provided below. In the results section (Section 3.4), there would be a change in line number 6, wherein the word ‘extrinsic’ would be replaced by the word ‘intrinsic’. The authors would like to apologise for any inconvenience caused.[Figure presented] Caption: Fig. 4A. Schematic representation of the intrinsic coagulation pathway and the role of Factor VIII in enhancing blood clotting. © 2025 Elsevier B.V.
Development of multimodal silver thiocyanate nanoparticles for the treatment of infected wounds in animal model
(Elsevier B.V., 2025) Malay Nayak; Avinash Mishra; Durba Banerjee; Rajdeep Bhattacharjee; Anjali Upadhyay; Ashish Kumar Kumar Agrawal; Sudip Mukherjee
Wound healing, a complex process often impaired by infection and chronic conditions, necessitates innovative therapeutic strategies. To address this, we investigated silver thiocyanate nanoparticles (AgSCN-NPs) as a multimodal agent for infection control and wound care. AgSCN-NPs displayed potent anti-infective properties against gram-positive (Bacillus subtilis) and gram-negative (E. coli) bacteria. Specifically, zone of inhibition assays revealed significantly larger inhibition zones for AgSCN-NPs (21 mm for B. subtilis and 18 mm for E. coli) compared to amoxicillin (10 mm for both strains) at 100 µg/mL. Confocal microscopy showed that AgSCN-NPs effectively disrupted E. coli biofilm formation. In vivo, studies in a rat model demonstrated that AgSCN-NPs (100 µg/mL) accelerated wound closure, with AgSCN-NP treated wounds achieving 98.151 ± 0.87 % closure by day 12, compared to the untreated control group (89.473 ± 4.96 %). Immunohistochemical staining revealed increased expression of CD163 (five-fold increase) and α-SMA (2.7-fold increase) in AgSCN-NP-treated tissues, indicating enhanced anti-inflammatory activity and tissue regeneration. Moreover, infected wound healing studies were performed using E. coli infection demonstrating rapid healing of infected wounds in the AgSCN-NP treatment. These findings suggest that AgSCN-NPs offer a promising multimodal approach to enhance wound healing and combat skin infections associated with wound healing. © 2025 Elsevier B.V.
Engineered cell-laden hemostatic hydrogels for all-round rapid repair of diabetic wounds
(Elsevier B.V., 2025) Malay Nayak; Durba Banerjee; Devyani Yenurkar; Snehasish Mandal; Lipi Pradhan; Arnab Sarkar; Sudip Mukherjee
The complex microenvironment of diabetic wounds, characterized by persistent inflammation, impaired angiogenesis, and compromised hemostasis, severely delays healing and presents a significant clinical challenge. Current therapies often lack the multifaceted approach required to address these interwoven pathologies effectively. Herein, we present a novel cell-based therapeutic platform: precision-engineered human embryonic kidney (HEK 293T) cells encapsulated within alginate hydrogels, designed for diabetic wound repair and liver hemostasis. HEK 293T cells are stably engineered via a PiggyBac transposon system to co-express pro-angiogenic human Vascular Endothelial Growth Factor A (hVEGFA), hemostatic Factor VIII (hFVIII), and immunomodulatory Interleukin-10 (hIL10), ensuring sustained secretion of these therapeutic factors. In vitro studies confirmed hVEGFA-mediated elevation of cell migration and wound closure, hFVIII stimulated acceleration of blood clotting, and hIL10's role as a potent anti-inflammatory agent, reducing oxidative stress. A combination of hVEGFA, hFVIII, and hIL10 notably achieved superior in vitro and in vivo wound healing. In a diabetic rat full-thickness excisional wound model, administration of these engineered cell-laden hydrogels significantly accelerated wound closure within 2 weeks. Furthermore, cell-secreted hFVIII demonstrated remarkable hemostatic efficacy when mixed with human blood ex vivo and in a liver puncture model, reducing activated partial thromboplastin time, blood loss, and clotting time in vivo. On the other hand, capsules did not show any xenogeneic effect and immunogenicity in the long term. This multi-functional cell-based hydrogel system offers a comprehensive and promising platform for rapid attenuation of homeostasis for rapid diabetic wound healing. © 2025 Elsevier B.V.
Neurogenic and angiogenic poly(N-acryloylglycine)-co-(acrylamide)-co-(N-acryloyl-glutamate) hydrogel: preconditioning effect under oxidative stress and use in neuroregeneration
(Royal Society of Chemistry, 2024) Kirti Wasnik; Prem Shankar Gupta; Gurmeet Singh; Somedutta Maity; Sukanya Patra; Divya Pareek; Sandeep Kumar; Vipin Rai; Ravi Prakash; Arbind Acharya; Pralay Maiti; Sudip Mukherjee; Yitzhak Mastai; Pradip Paik
Traumatic injuries, neurodegenerative diseases and oxidative stress serve as the early biomarkers for neuronal damage and impede angiogenesis and subsequently neuronal growth. Considering this, the present work aimed to develop a poly(N-acryloylglycine)-co-(acrylamide)-co-(N-acryloylglutamate) hydrogel [p(NAG-Ac-NAE)] with angiogenesis/neurogenesis properties. As constituents of this polymer modulate their vital role in biological functions, inhibitory neurotransmitter glycine regulates neuronal homeostasis, and glutamatergic signalling regulates angiogenesis. The p(NAG-Ac-NAE) hydrogel is a highly branched, biodegradable and pH-responsive polymer with a very high swelling behavior of 6188%. The mechanical stability (G′, 2.3-2.7 kPa) of this polymeric hydrogel is commendable in the differentiation of mature neurons. This hydrogel is biocompatible (as tested in HUVEC cells) and helps to proliferate PC12 cells (152.7 ± 13.7%), whereas it is cytotoxic towards aggressive cancers such as glioblastoma (LN229 cells) and triple negative breast cancer (TNBC; MDA-MB-231 cells) and helps to maintain the healthy cytoskeleton framework structure of primary cortical neurons by facilitating the elongation of the axonal pathway. Furthermore, FACS results revealed that the synthesized hydrogel potentiates neurogenesis by inducing the cell cycle (G0/G1) and arresting the sub-G1 phase by limiting apoptosis. Additionally, RT-PCR results revealed that this hydrogel induced an increased level of HIF-1α expression, providing preconditioning effects towards neuronal cells under oxidative stress by scavenging ROS and initiating neurogenic and angiogenic signalling. This hydrogel further exhibits more pro-angiogenic activities by increasing the expression of VEGF isoforms compared to previously reported hydrogels. In conclusion, the newly synthesized p(NAG-Ac-NAE) hydrogel can be one of the potential neuroregenerative materials for vasculogenesis-assisted neurogenic applications and paramount for the management of neurodegenerative diseases. © 2024 The Royal Society of Chemistry.
Poly(N-acryloylglycine-acrylamide) Hydrogel Mimics the Cellular Microenvironment and Promotes Neurite Growth with Protection from Oxidative Stress
(American Chemical Society, 2023) Kirti Wasnik; Prem Shankar Gupta; Sudip Mukherjee; Alagu Oviya; Ravi Prakash; Divya Pareek; Sukanya Patra; Somedutta Maity; Vipin Rai; Monika Singh; Gurmeet Singh; Desh Deepak Yadav; Santanu Das; Pralay Maiti; Pradip Paik
In this work, the glycine-based acryloyl monomer is polymerized to obtain a neurogenic polymeric hydrogel for regenerative applications. The synthesized poly(N-acryloylglycine-acrylamide) [poly(NAG-b-A)] nanohydrogel exhibits high swelling (∼1500%) and is mechanically very stable, biocompatible, and proliferative in nature. The poly(NAG-b-A) nanohydrogel provides a stable 3D extracellular mimetic environment and promotes healthy neurite growth for primary cortical neurons by facilitating cellular adhesion, proliferation, actin filament stabilization, and neuronal differentiation. Furthermore, the protective role of the poly(NAG-b-A) hydrogel for the neurons in oxidative stress conditions is revealed and it is found that it is a clinically relevant material for neuronal regenerative applications, such as for promoting nerve regeneration via GSK3β inhibition. This hydrogel additionally plays an important role in modulating the biological microenvironment, either as an agonist and antagonist or as an antioxidant. Furthermore, it favors the physiological responses and eases the neurite growth efficiency. Additionally, we found out that the conversion of glycine-based acryloyl monomers into their corresponding polymer modulates the mechanical performance, mimics the cellular microenvironment, and accelerates the self-healing capability due to the responsive behavior towards reactive oxygen species (ROS). Thus, the p(NAG-b-A) hydrogel could be a potential candidate to induce neuronal regeneration since it provides a physical cue and significantly boosts neurite outgrowth and also maintains the microtubule integrity in neuronal cells. © 2023 American Chemical Society.
Protocatechuic Acid and its Derivatives: Synthesis, Antioxidant Properties, and Potential In Vivo Wound Healing Applications
(John Wiley and Sons Ltd, 2025) Sumit Maurya; Sumit Manna; Mala Singh; Dharmendra Singh; Malay Nayak; Sudip Mukherjee; Arun Kumar Manna
Treatment of chronic wounds is a potential healthcare problem, affecting people globally. Traditional methods of wound healing are prone to several limitations, like infections, oxidative stress, and development of secondary wounds. Management of oxidative stress is an interesting platform to deal with chronic wounds. For this study, novel derivatives of protocatechuic acid, a naturally occurring phenolic acid, were designed by modifying the carboxylic acid moiety while preserving the hydroxyl groups for radical scavenging. So, a series of amide conjugates were synthesized by incorporating various amine moieties. Glucose conjugates were obtained through both click chemistry and direct coupling strategies and a chlorinated derivative was also prepared. The antioxidant potential of the compounds was screened using DPPH assay, followed by in vitro DCFDA assay on HEK-293T cell line. Top 5 lead molecules were checked for biocompatibility through MTT assay, which provided us with top 2 leads, Compounds 12 and 17 which were examined for a wound healing study on Wistar rats along with the starting compound 1. It was observed that Compound 17 demonstrated excellent wound tissue regeneration on day 12, as compared to the control group, suggesting the promising role of triazole and glucose moieties conjugation for relieving oxidative stress and wound management. © 2025 Wiley-VCH GmbH.
Recent advances of nanocrystals in cancer theranostics
(Royal Society of Chemistry, 2023) Devyani Yenurkar; Malay Nayak; Sudip Mukherjee
Emerging cancer cases across the globe and treating them with conventional therapies with multiple limitations have been challenging for decades. Novel drug delivery systems and alternative theranostics are required for efficient detection and treatment. Nanocrystals (NCs) have been established as a significant cancer diagnosis and therapeutic tool due to their ability to deliver poorly water-soluble drugs with sustained release, low toxicity, and flexibility in the route of administration, long-term sustainable drug release, and noncomplicated excretion. This review summarizes several therapies of NCs, including anticancer, immunotherapy, radiotherapy, biotheranostics, targeted therapy, photothermal, and photodynamic. Further, different imaging and diagnostics using NCs are mentioned, including imaging, diagnosis through magnetic resonance imaging (MRI), computed tomography (CT), biosensing, and luminescence. In addition, the limitations and potential solutions of NCs in the field of cancer theranostics are discussed. Preclinical and clinical data depicting the importance of NCs in the spotlight of cancer, its current status, future aspects, and challenges are covered in detail. © 2023 RSC.