Browsing by Author "Sujata Roy"

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In situ patterns of nuclear replication in brain ganglia of l(2) gl4 mutant larvae of Drosophila melanogaster
(1991) Sujata Roy; S.C. Lakhotia
The 1(2) gl4 mutation (a deletion mutation of a recessive oncogene) of Dwsophila melanogaster causes neuroblastoma in the optic centres of brain of late third instar larvae. We have studied the in situ patterns of DNA synthesis in these brains by immunocytochemical detection of cells incorporating 5-bromodeoxyuridine. It was seen that 1 (2) gl4 brains from younger 3rd instar larvae had fewer replicating cells than in wild type larvae of comparable age but in brain ganglia of older l (2) gl4 larvae the number of replicating cells was much higher. The spatial distribution of replicating cells in optic lobes of brain ganglia of 1 (2) gl4 larvae was disturbed from early 3rd instar stage, much before the tumourous growth was morphologically detectable. The stereotyped pattern of asymmetrical cell divisions of the neuroblasts and their progeny cells was also not seen in 1 (2) gl4 brain ganglia. Therefore, it appears that the l (2)gl4 product has an important role early in development to determine the temporal and spatial patterns of neuroblast cell division in developing brains. © 1991, Indian Academy of Sciences. All rights reserved.