Browsing by Author "Sumit Kumar Anand"

Now showing 1 - 2 of 2

Green synthesis of silver nanoparticles by root extract of Premna integrifolia L. and evaluation of its cytotoxic and antibacterial activity
(Elsevier Ltd, 2023) Chandrashekhar Singh; Sumit Kumar Anand; Richa Upadhyay; Nidhi Pandey; Pradeep Kumar; Deepjyoti Singh; Punit Tiwari; Rajesh Saini; Kavindra Nath Tiwari; Sunil Kumar Mishra; Ragini Tilak
An efficient, reproducible, and eco-friendly protocol has been developed for the green synthesis of silver nanoparticles using an aqueous root extract of Premna integrifolia (PE-AgNPs). The synthesized nanoparticles were characterized by different techniques to determine their formation, stability, and morphology. Nanoparticles were tested for cytotoxicity against the HepG2 cancer cell line and antibacterial activity against clinical isolates of bacteria. The cytotoxic potential of PE-AgNPs was analyzed using MTT assay, Hoechst and AO/EtBr staining, ROS measurement, mitochondrial membrane potential, clonogenic, and wound healing assays. PE-AgNPs were found strongly cytotoxic against Hep G2 cell lines during all assays. A disc diffusion assay confirmed that Pseudomonas aeruginosa was most susceptible to PE-AgNPs than Staphylococcus aureus and E. coli. It was further confirmed by MIC, MBC values, and TEM microphotographs. As a result, our findings indicate that biosynthesized silver nanoparticles could be useful as cytotoxic and antimicrobial candidates. © 2023 Elsevier B.V.
Phytochemical profiling and cytotoxic evaluation of Premna serratifolia L. against human liver cancer cell line
(Springer Science and Business Media Deutschland GmbH, 2021) Chandrashekhar Singh; Sumit Kumar Anand; Kavindra Nath Tiwari; Sunil Kumar Mishra; Poonam Kakkar
Premna serratifolia L. (Lamiaceae) is a medicinal plant, widely distributed in the tropical and subtropical regions and commonly used in traditional medicine. The current study was focused to evaluate the cytotoxic potential of aqueous extract of root of P. serratifolia (AEPS) against human hepatoblastoma cancer cell line (Hep G2).The yield of the dried extract was 5.8% and used for further studies.Cytotoxic potential of AEPS was analyzed by MTT assay, which exhibits IC50 value 1000 µg/mL after 48 h incubation. Hoechst and AO/EtBr staining, ROS measurement, mitochondrial membrane potential, clonogenic and wound healing assays also confirmed the cytotoxic efficacy of AEPS in dose and time-dependent manner. UPLC–Q-TOF–MS/MS analysis of AEPS confirmed the presence of 12polyphenolic compounds, namely 4-hydroxy-3-methoxycinnamic acid, linarin, peonidin-3,5-O-di-beta-glucopyranoside, diosmin, trans-cinnamic acid, daidzein, saponarin, homoorietin, acacetin, sarsasapogenin, phytol and sissotrin. The cytotoxic potential of AEPS might due to presence of biologically active polyphenolic compounds. © 2021, King Abdulaziz City for Science and Technology.