Browsing by Author "Sumit Kumar Pandey"

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A comparative study of band gap engineered in-situ and ex-situ MWCNTs/TiO2 heterostructures for their enhanced photocatalytic activity under visible light
(Elsevier B.V., 2023) Anshu Kumar Singh; Pramod Kumar Vishwakarma; Sumit Kumar Pandey; Raghvendra Pratap; Rajiv Giri; Anchal Srivastava
Organic pollutants, such as various types of organic dyes coming out from the textile industries, are polluting surface and groundwater resources alarmingly and posing a threat to aquatic ecosystems. So, the demand for visible-light-driven high-performance photocatalysts having high activity and structural stability is a need of an hour. TiO2 has been one of the well-known and most studied semiconductor photocatalysts for decades. But its low electron-hole pair (e-/h+) recombination time reduces its efficiency, and the large band gap restricts its use as a visible-light-driven photocatalyst. To overcome these limitations of TiO2, herein, we have reported an in-situ and ex-situ MWCNTs modified TiO2 heterostructure nanocomposites photocatalyst and established a comparative study in terms of their ability to degrade methylene blue (MB) dye under visible light irradiation. The as-synthesized in-situ CNTs-TiO2 nanocomposite and ex-situ CNTs-TiO2 nanocomposite were characterized structurally, morphologically, compositionally, and optically through various characterization techniques such as XRD, RAMAN, SEM, XPS, FTIR, and UV–Vis diffuse reflectance spectroscopy. The result reveals the band gap tuning in the in-situ and ex-situ CNTS-TiO2 nanocomposites as a result of increasing MWCNTs concentration. The in-situ CNTs-TiO2-2 nanocomposite has high degradation efficiency (94% in 150 min) and stability due to smooth and strong chemical interactions between the MWCNTs and TiO2, while ex-situ CNTs-TiO2-20 with 10 times more MWCNTs concentration (by weight) as compared to MWCNTs concentration in in-situ CNTs-TiO2-2, exhibits degradation efficiency of 89% in 150 min. The possible degradation mechanism to degrade MB dye has also been put forward. © 2023
Carbon nanotubes molybdenum disulfide 3D nanocomposite as novel nanoscaffolds to immobilize Lens culinaris β-galactosidase (Lsbgal): Robust stability, reusability, and effective bioconversion of lactose in whey
(Elsevier Ltd, 2019) Anjali Yadav; Sumit Kumar Pandey; Dinesh Chand Agrawal; Himanshu Mishra; Anchal Srivastava; Arvind M. Kayastha
Multiwalled carbon nanotubes molybdenum disulfide 3D nanocomposite (MWCNT-MoS2 NC) was successfully synthesized via eco-friendly hydrothermal method. The microstructural characterization of synthesized nanocomposite was carried out using different spectroscopic and microscopic techniques. Nanocomposite was activated using glutaraldehyde chemistry and used as a platform to immobilize Lens culinaris β-galactosidase (Lsbgal) which resulted in 93% of immobilization efficiency. Attachment of Lsbgal onto nanocomposite was confirmed by AFM, FE-SEM, FTIR, and CLSM. The nanobiocatalyst showed broadening in operational pH and temperature working range. Remarkable increase in thermal stability was observed as compared to soluble enzyme. Nanobiocatalyst showed outstanding increase in storage stability, retained 92% of residual activity over a period of 8 months. This offers good reusability as it retained ∼50% residual activity up to 21 reuses and exhibited higher rate of lactose hydrolysis in whey. MWCNT-MoS2 NC conjugated to biomolecules can serve as a potential platform for fabrication of lactose biosensor. © 2019 Elsevier Ltd
Catalyst-free biphasic orthorhombic/hexagonal tungsten oxide system with enhanced photocatalytic response under visible light
(Oxford University Press, 2023) Anshu Kumar Singh; Sumit Kumar Pandey; Pramod Kumar Vishwakarma; Raghvendra Pratap; Ranjana Verma; Anamika Pandey; Rajiv Giri; Anchal Srivastava
These days, textile industries pose a more significant threat to surface water and groundwater sources directly or indirectly by discharging wastewater containing various dyes and organic pollutants to these water sources. The phase-junction-engineered heterogeneous photocatalysis carried out by visible light-driven semiconductor photocatalysts is opening a new window for the degradation of environmental organic pollutants. In this work, we have reported a one-step bottom-up hydrothermal synthesis of biphasic tungsten oxide (o/h-WO3) and performed a photodegradation experiment under visible light irradiation for the efficient degradation of organic pollutants such as Methylene blue (MB) and Methyl violet (MV), respectively. The X-ray diffraction, RAMAN, transmission electron microscopy and ultraviolet-Vis characterization techniques were used to investigate the structural, morphological and optical properties of the as-synthesized o/h-WO3. Moreover, the low calculated band gap (∼2.8 eV) and the anionic nature of o/h-WO3 suggest it as an efficient visible light-driven photocatalyst suitable for heterogeneous photocatalysis. The photodegradation experiment performed under visible light using o/h-WO3 photocatalyst showed better degradation efficiency of 71% and 89% for MB and MV, respectively, in 100 min. The dyes followed first-order kinetics, and their kinetic rate constants were calculated using the Langmuir-Hinshelwood model. Furthermore, the recyclability study of the photocatalyst was also performed and discussed the underlying mechanism for the photodegradation of the organic dyes. © 2023 The Author(s).
Characterizations of nanoscale two-dimensional materials and heterostructures
(Elsevier, 2020) Anchal Srivastava; Chandra Shekhar Pati Tripathi; Vijay Kumar Singh; Rohit Ranjan Srivastava; Sumit Kumar Pandey; Suyash Rai; Ravi Dutt; Amit Kumar Patel
In recent years, two-dimensional (2D) atomically thin crystals ranging from insulator to superconductor such as graphene, hexagonal boron nitride (h-BN), transition metal dichalcogenides (TMDs), etc. have attracted extensive attention due to their exceptional properties and many potential applications in various areas. In this chapter we focus on the experimental characterization of 2D materials and their heterostructures andcover brief introduction and detailed structural, optical, and chemical characterizations of some important 2D materials. © 2020 Elsevier Inc.
Correction to: First report of edible mushroom Pleurotus ostreatus from India with potential to kill plant parasitic nematodes (Indian Phytopathology, (2019), 72, 1, (173-176), 10.1007/s42360-018-0093-0)
(Springer, 2019) R.K. Singh; Sumit Kumar Pandey; Dalel Singh; Prahlad Masurkar
The original version of this article was revised: The article First report of edible mushroom Pleurotusostreatus from India with potential to kill plant parasitic nematodes, was written by R. K. Singh, Sumit Kumar Pandey, Dalel Singh, and Prahlad Masurkar, was originally published electronically on the publisher’s internet portal (currently SpringerLink) on 14 December 2018 with open access. With the author decision to step back from Open Choice, the copyright of the article changed in April 2019 to © Indian Phytopathological Society 2019 and the article is forthwirth distributed under the terms of copyright. © 2019, Indian Phytopathological Society.
Design of an efficient, tunable and scalable freestanding flexible membrane for filter application
(Royal Society of Chemistry, 2022) Sumit Kumar Pandey; Anchal Srivastava
To address the global challenge of water pollution, membrane-based technologies are being used as a dignified separation technology. However, designing low-cost, reusable, freestanding and flexible membranes for wastewater treatment with tunable pore size, good mechanical strength, and high separation efficiency is still a major challenge. Herein, we report the development of a scalable, reusable, freestanding, flexible and functionalized multiwalled carbon nanotube (FMWCNT) membrane filter with tunable pore size for wastewater treatment, which has attractive attributes such as high separation efficiency (>99% for organic dyes and ∼80% for salts), permeance (∼225 L h-1 m-2 bar-1), tensile strength (∼6 MPa), and reusability of both the membrane as well as contaminants separately. This FMWCNTs membrane filter has been developed by a simple vacuum-assisted filtration technique followed by the synthesis of MWCNTs using a cost-effective spray pyrolysis assisted chemical vapor deposition (CVD) technique and chemical functionalization. This study deals with understanding the rejection, retrieval, and reusability of both the membranes as well as waterborne contaminants separately. The developed membrane filter has potential utility in many applications such as wastewater treatment, food industry, and life sciences due to its robust mechanical and separation performance characteristics. © The Royal Society of Chemistry.
Detection and diagnosis of seed-borne and seed-associated nematodes
(Springer Singapore, 2020) R.K. Singh; Sumit Kumar Pandey; Anirudha Chattopadhyay
Seed is a very good carrier of phytonematodes, especially for long distance dissemination. This transmission occurs either directly through seed infection or via seed contamination. Although in low rate, this transmission becomes a serious concern when trans-boundary movement of invasive alien nematode species flares up in epiphytotic proportion. Hence, detection and diagnosis of seed-borne and seed-associated nematode are significant for their protection. So far, various conventional methods are mostly employed for detection, but advancement of modern approaches, viz. serological or molecular techniques, speeds up the process with more accurate detection. Their robustness and specificity with greater resolution help in identification and discrimination of different species of seed-borne phytonematodes, thus strengthening phytosanitary programme and ensuring low risk in world trade. It also helps in pest risk analysis (PRA) of any pests having quarantine importance and development of national standards for import of seed and planting materials. Besides these, for the identification of pest-free area, production of certified seeds, and promotion of export quality raw plant products, detection of seed and seed-associated nematodes is very essential. Hence, detection is always considered as primary step for crop protection. © Springer Nature Singapore Pte Ltd. 2020.
Fabrication of sensitive bioelectrode based on atomically thin CVD grown graphene for cancer biomarker detection
(Elsevier Ltd, 2018) Vijay K. Singh; Saurabh Kumar; Sumit Kumar Pandey; Saurabh Srivastava; Monu Mishra; Govind Gupta; B.D. Malhotra; R.S. Tiwari; Anchal Srivastava
Motivation behind the present work is to fabricate a cost effective and scalable biosensing platform for an easy and reliable detection of cancer biomarker Carcinoembryonic antigen (CEA). Here, we report the sensitive and selective detection of CEA using graphene based bio-sensing platform. Large sized (~ 2.5 × 1.0 cm2), uniform, continuous, single and few layers graphene films have been grown on copper (Cu) substrate employing chemical vapor deposition (CVD) technique using hexane as a liquid precursor. Functional group has been created over Graphene/Cu substrate through π-π stacking of 1- pyrenebutanoic acid succinimidyl ester (PBSE). Further, to make the sensor specific to CEA, antibody of CEA (anti-CEA) has been covalently immobilized onto PBSE/Graphene/Cu electrode. Selective and sensitive detection of CEA is achieved by anti-CEA/PBSE/Graphene/Cu electrode through electrochemical impedance spectroscopy (EIS) measurements. Under optimal condition, the fabricated sensor shows linear response in the physiological range 1.0–25.0 ng mL−1 (normal value ~ 5.0 ng mL−1), revealing sensitivity 563.4 Ω ng−1 mL cm−2 with a correlation coefficient of 0.996 and limit of detection (LOD) 0.23 ng mL−1. In this way, one step electrode fabrication with high specific surface area provides a light weight, low cost, reliable and scalable novel biosensing platform for sensitive and selective detection of CEA. We believe that this bioelectrode equipped with specific recognition elements could be utilized for detection of other biomolecules too. © 2018 Elsevier B.V.
First report of edible mushroom Pleurotus ostreatus from India with potential to kill plant parasitic nematodes
(Springer, 2019) R.K. Singh; Sumit Kumar Pandey; Dalel Singh; Prahlad Masurkar
Edible fungus Pleurotus ostreatus was, isolated cultured and identified from the bark of Mangifera indica tree. The hyphae emerging from the fruiting body produces secretory cells with toxin droplets in water agar medium which immobilize the nematode and mostly enter from the mouth region. Earlier finding showed that trans-2-decenedioic acid toxin affects only saprophytic nematodes through the mouth part but similar observations in plant parasitic nematode Meloidogyne incognita suggested that there is no obstacle from stylet in paralysing and killing. From Indian subcontinent P. ostreatus was first time isolated, in perspective of plant parasitic nematode management in addition to mushroom production. © 2018, The Author(s).
In Situ Fabrication of Activated Carbon from a Bio-Waste Desmostachya bipinnata for the Improved Supercapacitor Performance
(Springer, 2021) Gopal Krishna Gupta; Pinky Sagar; Sumit Kumar Pandey; Monika Srivastava; A.K. Singh; Jai Singh; Anchal Srivastava; S.K. Srivastava; Amit Srivastava
Herein, we demonstrate the fabrication of highly capacitive activated carbon (AC) using a bio-waste Kusha grass (Desmostachya bipinnata), by employing a chemical process followed by activation through KOH. The as-synthesized few-layered activated carbon has been confirmed through X-ray powder diffraction, transmission electron microscopy, and Raman spectroscopy techniques. The chemical environment of the as-prepared sample has been accessed through FTIR and UV–visible spectroscopy. The surface area and porosity of the as-synthesized material have been accessed through the Brunauer–Emmett–Teller method. All the electrochemical measurements have been performed through cyclic voltammetry and galvanometric charging/discharging (GCD) method, but primarily, we focus on GCD due to the accuracy of the technique. Moreover, the as-synthesized AC material shows a maximum specific capacitance as 218 F g−1 in the potential window ranging from − 0.35 to + 0.45 V. Also, the AC exhibits an excellent energy density of ~ 19.3 Wh kg−1 and power density of ~ 277.92 W kg−1, respectively, in the same operating potential window. It has also shown very good capacitance retention capability even after 5000th cycles. The fabricated supercapacitor shows a good energy density and power density, respectively, and good retention in capacitance at remarkably higher charging/discharging rates with excellent cycling stability. Henceforth, bio-waste Kusha grass-derived activated carbon (DP-AC) shows good promise and can be applied in supercapacitor applications due to its outstanding electrochemical properties. Herein, we envision that our results illustrate a simple and innovative approach to synthesize a bio-waste Kusha grass-derived activated carbon (DP-AC) as an emerging supercapacitor electrode material and widen its practical application in electrochemical energy storage fields. © 2021, The Author(s).
Moisture and nitrogen stress induces severity of wiltnematode complex of lentil grown in rice fallow in an inceptisol
(Indian Phytopathological Society, 2017) Raj Mukhopadhyay; Victor Phani; Nirmal De; Sumit Kumar Pandey; Rakesh Kumar Singh
Lentil (Lens culinaris) is a leading high protein pulse grown by the farmers as a rainfed rabi crop in lowland rice fallows in Indo Gangetic Plains (IGP) of India. Disease complex due to Fusarium oxysporum and Meloidogyne incognita possess a great threat to yield of lentil when grown as fallow crop after rice with residual soil moisture and nitrogen in dryland condition. This paper discusses the role of soil moisture and availability of soil NO3-N to the plants on wilt nematode disease complex under a long term rice-lentil cropping system. In our long term experimental conditions which prevailed under dry condition, low level of soil moisture and low availability of soil NO3-N to plants illustrated increased in fungus nematode wilt complex severity, which can be mitigated by use of farm yard manure as full source of nitrogen in rice. This leads to the enhancement of the available soil moisture and nitrogen for residual crops like lentil and also reduced wilt nematode complex. © Indian Phytopathological Society 2017.
Multiwalled Carbon Nanotube Filters for Toxin Removal from Cigarette Smoke
(American Chemical Society, 2020) Sumit Kumar Pandey; Pramod Kumar Vishwakarma; Sunil Kumar Yadav; Prashant Shukla; Anchal Srivastava
The fatal diseases and disorders caused by smoking have raised a serious concern for worldwide public health. So for its prevention, it has become very necessary to develop various types of filter material for the removal of carcinogenic and other toxic elements present in cigarette smoke. Herein, we demonstrate a novel filter for cigarettes, fabricated by the insertion of multiwalled carbon nanotubes (MWCNTs) based thin flexible membrane into the conventional cellulose acetate filter. The developed filter has attractive attributes of high filtering efficiency, lightweight, flexible, cost-effective, and scalable production. This flexible MWCNTs membrane has been fabricated using a simple vacuum-assisted filtration technique followed by the synthesis of MWCNTs using a cost-effective spray pyrolysis technique. The filter shows excellent performance for the removal of PM2.5, having a removal efficiency of ∼99%. It also shows a significant ability to remove nicotine, tar, and toxic heavy metals such as lead present in cigarette smoke. Copyright © 2019 American Chemical Society.
Multiwalled Carbon Nanotube-Based Freestanding Filters for Efficient Removal of Fine Particulate Matters (PM0.3), Microplastics (MP0.3), and Bioaerosols
(American Chemical Society, 2022) Pramod Kumar Vishwakarma; Sumit Kumar Pandey; Sunil Kumar Yadav; P. Shukla; Anchal Srivastava; Rajiv Giri
Air pollution caused by various hazards such as particulate matters (PM), microplastics (MP), bioaerosols (BA), etc. has become a global concern for public health across the globe. In recent decades, nanofiltration-based air purification techniques have rapidly evolved as a viable solution to address worldwide air pollution challenges. Herein, we report a simple, cost-effective, and scalable technique for the fabrication of lightweight, freestanding, and flexible multiwalled carbon nanotube (MWCNT) membranes for air filter applications. The developed membrane filter possesses excellent capturing efficiency of more than 99% for PM0.3, MP0.3, and BA. Additionally, the developed membrane has also been well investigated in terms of hydrophobic behavior (contact angle ∼148 ± 7°), narrow pore size (∼16 nm), packing density (∼0.65 g/cc), porosity (∼56%), pressure drop (∼139.7 Pascal), flexibility, and reusability, reflecting its self-cleaning feature, physical sieve characteristics, adaptability, and commercialization. Thus, the filter developed in this work shows its potential utility toward the removal of indoor pollution and in air filter industries as well. © 2022 American Chemical Society.
Reusable and thermostable multiwalled carbon nanotubes membrane for efficient removal of benz[α]anthracene from cigarette smoke
(Oxford University Press, 2023) Pramod Kumar Vishwakarma; Sumit Kumar Pandey; Anshu Kumar Singh; Sanny Rathore; Kirpa Ram; Rajiv Giri; Anchal Srivastava
Poly-aromatic hydrocarbons (PAHs), such as benz[α]anthracene (BaA), are often released from cigarette smoke and pose a severe threat to public health. In this quest, several filtration techniques have been proposed for the elimination of these toxins, but the challenges remain the same in terms of low efficiency, high cost and complex manufacturing processes. Herein, we report a simple, cost-effective and reusable multiwalled carbon nanotubes (MWCNTs) membrane-based filter for enhanced removal (∼90%) of BaA from cigarette smoke. In terms of the excellent thermal stability of the MWCNTs membrane, the adsorbed PAHs could be removed simply by a thermolysis process. We believe that the present study may serve as inspiration for the design and development of high-performance fibrous materials for filtering and separation applications. © 2023 The Author(s). Published by Oxford University Press.
Rhizobacteria–Plant Interaction, Alleviation of Abiotic Stresses
(Springer, 2019) R.K. Singh; Prahlad Masurkar; Sumit Kumar Pandey; Suman Kumar
At the present scenario, climate change became the potential threat to growers with rise in temperature, inconsistent rainfall, and salinization of agricultural land. However, the microbes more specifically plant growth-promoting rhizobacteria (PGPR) play a significant role to mitigate the abiotic stresses. Rhizobacteria act as bioprotectants against drought, salt, heavy metals, high temperature, and cold stress. During drought condition, PGPR intensifies osmolytes (proline, glycine, betaine) and acts as an osmoprotectant. The drought-related enzyme ACC deaminases were regulated by the PGPR, which also regulates the stomatal physiology during the water deficit conditions. The salt stress in plants was also a complex process to understand. During salt stress condition, PGPR acts as an activator of antioxidant enzymes and polyamines and also acts as a modulator of abscisic acid. Inoculation of PGPR affects the expression of 14 genes (four upregulated and two downregulated) related to salt stress. The effect of heavy metal toxicity is also found in plants, which is due to the improper fertilizer applications, industrial waste, sludge, etc. The main site for accumulation of heavy metals is the root nodule. At present many PGPR sp., i.e., Bacillus sp., Pseudomonas sp., Azotobacter sp., Enterobacter sp., and Rhizobium sp., were proposed to speed up the phytoremediation process of nodules. Bacterial metallothioneins (MTs) of the family Bmt, a family with low-molecular proteins, play a significant role to absorb heavy metals. High temperature also acts as a constraint of normal plant root nodulation and rhizobial growth. The strains of PGPRs evolve during the heat stress period against the raised temperature with the production of extra LPS, EPS, and special class of proteins, i.e., heat shock proteins (HSPs). Cold tolerance can also be derived by PGPR as the accumulation of more carbohydrate, regulation of stress-related genes for osmolytes expression, and enhancement of specific protein synthesis, which helps plant to fight against cold stress. © Springer Nature Singapore Pte Ltd. 2019.
Silica Nanospheres Coated Silver Islands as an Effective Opto-Plasmonic SERS Active Platform for Rapid and Sensitive Detection of Prostate Cancer Biomarkers
(MDPI, 2022) Anamika Pandey; Subhankar Sarkar; Sumit Kumar Pandey; Anchal Srivastava
The in vitro diagnostics of cancer are not represented well yet, but the need for early-stage detection is undeniable. In recent decades, surface-enhanced Raman spectroscopy (SERS) has emerged as an efficient, adaptable, and unique technique for the detection of cancer molecules in their early stages. Herein, we demonstrate an opto-plasmonic hybrid structure for sensitive detection of the prostate cancer biomarker sarcosine using silica nanospheres coated silver nano-islands as a facile and efficient SERS active substrate. The SERS active platform has been developed via thin (5–15 nm) deposition of silver islands using a simple and cost-effective Radio Frequency (RF) sputtering technique followed by the synthesis and decoration of silica nanospheres (~500 nm) synthesized via Stober’s method. It is anticipated that the coupling of Whispering Gallery Modes and photonic nano-jets in SiO2 nanospheres induce Localized Surface Plasmon Resonance (LSPR) in Ag nano-islands, which is responsible for the SERS enhancement. The as-fabricated SERS active platform shows a linear response in the physiological range (10 nM to 100 μM) and an extremely low limit of detection (LOD) of 1.76 nM with a correlation coefficient of 0.98 and enhancement factor ~2 × 107. The findings suggest that our fabricated SERS platform could be potentially used for the rapid detection of bio-chemical traces with high sensitivity. © 2022 by the authors.
Thermally Stable, Thin, Ultralight, Reusable, and Flexible Multiwalled Carbon Nanotube Membranes for Removal of Heavy Metals, Polycyclic Aromatic Hydrocarbons, and Particulates from Coal Smoke
(American Chemical Society, 2023) Pramod Kumar Vishwakarma; Sumit Kumar Pandey; Anshu Kumar Singh; Sunil Kumar Yadav; P. Shukla; Sanny Rathore; Kirpa Ram; Preeti S. Saxena; Rajiv Giri; Anchal Srivastava
Rapid industrialization, haphazardous urbanization, and ruthless burning of fossil fuel have alarmingly impacted human health and the environment. The majority of health issues, such as asthma, cardiovascular disease, and cancer, are primarily associated with polyaromatic hydrocarbons (PAHs), particulate matter (PM), nickel (Ni) and cadmium (Cd) heavy metals, and other contaminants. To address these challenges, the development of an efficient air filtration unit to remove the toxic substances from the smoke is a necessity. The majority of the recently developed air filters are architecturally monotonous and bulky and suffer from a trade-off between removal effectiveness and air permeability. Herein, we have proposed an air filter which is scalable, ultralight (∼0.0034 g), thin (∼50 μm), thermally stable (∼500 °C), hydrophobic (contact angle 138° ± 6°), and porous (porosity of ∼57%) with a pore size of ∼16 nm and has an effective packing density of ∼0.64 g/cm3. The self-assembled nanoarchitecture network (nanonetwork) air filter has a relatively lower pressure drop (∼133.3 Pa) and can remove ∼99%, ∼99%, and ∼90% and 50% for PAHs, PM, and Ni and Cd heavy metals, respectively. This research will be highly beneficial for designing and developing high-performance fibrous materials for filtering and separation applications. © 2023 American Chemical Society.
Ultra-sensitive electrochemical detection of glutamate based on reduced graphene oxide/Ni foam nanocomposite film fabricated via electrochemical exfoliation technique using waste batteries graphite rods
(Elsevier Inc., 2024) Rim M. Alsharabi; Sumit Kumar Pandey; Jay Singh; Arvind M. Kayastha; Preeti S. Saxena; Anchal Srivastava
Glutamate (Glut) is the most abundant excitatory neurotransmitter in our brain and central nervous system (CNS) that is essential for the proper functioning of the brain. The uneven concentration of glutamate causes cell's overstimulation which leads to many severe diseases, including brain cancer, and brain cell damage. Further, the overexcitation of neuronal cells which is involved in the neurotoxic processes induces many neurodegenerative diseases such as amyotrophic lateral sclerosis, motor neuron, Huntington's, Alzheimer's, and Parkinson's diseases. Thus, real-time quantitative analysis is of utmost importance for its prevention. In the present study, we report an enzymatic electrochemical biosensor based on reduced graphene oxide-modified nickel foam (rGO/NiF) for glutamate detection. Graphene has been successfully synthesized via electrochemical exfoliation technique using waste batteries graphite rods as a cheap, waste recycling, and environmentally friendly approach. Using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), the rGO-modified NiF electrode demonstrates improved electrocatalytic activities compared to the bare Ni foam electrode. Under the optimal conditions, the fabricated enzyme-based rGO/NiF biosensor shows a linear response in the physiological range of 5–300 µM (normal value 30–80 µM), revealing a sensitivity of ∼4.8 µA/(µM·cm2) with a correlation coefficient of ∼0.997, the limit of detection (LOD) ∼0.1 µM and shelf-life of about ten weeks. Thus, we believe that the fabrication of this sensitive and selective responding biosensor will also enable us to measure the dynamic processes associated with Glut neurotransmission in the CNS. © 2024