Browsing by Author "Surajita Sahu"

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3D nanocomposites of β-TCP-H3BO3-Cu with improved mechanical and biological performances for bone regeneration applications
(Nature Research, 2025) Sarvesh Kumar Avinashi; Rajat Kumar Mishra; n. Shweta; Saurabh Kumar; Amreen Shamsad; Shama Parveen; Surajita Sahu; Savita Kumari; Zaireen Fatima; Sachin Kumar Yadav; Monisha Banerjee; Monalisa Mishra; Neeraj Mehta; Chandkiram Gautam
Recently, 3-D porous architecture of the composites play a key role in cell proliferation, bone regeneration, and anticancer activities. The osteoinductive and osteoconductive properties of β-TCP allow for the complete repair of numerous bone defects. Herein, β-TCP was synthesized by wet chemical precipitation route, and their 3-D porous composites with H3BO3 and Cu nanoparticles were prepared by the solid-state reaction method with improved mechanical and biological performances. Several characterization techniques have been used to investigate the various characteristics of fabricated porous composites. SEM and TEM studies revealed the porous morphology and hexagonal sheets of the β-TCP for the composite THC8 (82TCP-10H3BO3-8Cu). Moreover, the mechanical study showed excellent compressive strength (188 MPa), a high Young’s modulus (2.84 GPa), and elevated fracture toughness (9.11 MPa.m1/2). An in vitro study by MTT assay on osteoblast (MG-63) cells demonstrated no or minimal cytotoxicity at the higher concentration, 100 µg/ml after 24 h and it was found a more pronounced result at 20 µg/ml on increasing the concentration of Cu nanoparticles after incubating 72 h. The THC12 composite showed the highest antibacterial potency exclusively against B. subtilis. S. pyogene, S. typhi and E. coli. at 10 mg/ml, indicating its potential effectiveness in inhibiting all of these pathogens. Genotoxicity and cytotoxicity tests were also performed on rearing Drosophila melanogaster, and these findings did not detect any trypan blue-positive staining, which further recommended that the existence of composites did not harm the larval gut. Therefore, the fabricated porous composites THC8 and THC12 are suitable for bone regrowth without harming the surrounding cells and protect against bacterial infections. © The Author(s) 2025.
A novel nanocomposite of HAp-TiC-Ag with enhanced mechanical and biological properties for bone regrowth and anticancer applications
(Royal Society of Chemistry, 2025) Sarvesh Kumar Avinashi; Shweta; Rajat Kumar Mishra; Saurabh Kumar; Amreen Shamsad; Shama Parveen; Surajita Sahu; Savita Kumari; Zaireen Fatima; Vijay Pratap; Rupesh Kumar; Monisha Banerjee; Monalisa Mishra; Horesh Kumar; Rakesh Kumar C. Gautam; Chandkiram Gautam
Hydroxyapatite (HAp)-based composites are extensively used in various applications, including bone regeneration, bone implants, catalysis, drug delivery, and cancer treatment, owing to their unique properties such as osteogenesis, osteoconduction, and osteoinduction, as well as their ability to inhibit tumor cell growth. In this study, pure HAp and silver (Ag) nanoparticles were synthesized using microwave irradiation and green synthesis methods, while a solid-state reaction route was employed for the fabrication of HAp-TiC-Ag composites aimed at enhancing their mechanical and biological properties. A range of characterization techniques, including XRD, FTIR, Raman, XPS, DLS, SEM, TEM, and in vitro assays, were used to assess the structural, morphological, mechanical, and biological properties of the composites. The composite HTA6 exhibited excellent mechanical properties, including a high compressive strength (185 MPa), elevated fracture toughness (10.88 MPa m1/2), a moderate Young's modulus (1.08 GPa), and a Vickers hardness (339.65 HV). The cell viability tests demonstrated that HTA6 treatment did not significantly reduce osteoblast cell growth, while significantly inhibiting the proliferation of cancer cells. Additionally, the composite showed good biocompatibility, displaying non-cytotoxicity in D. melanogaster and strong antibacterial activities against the tested bacteria. These findings suggest that HTA6 is a promising candidate for applications in bone regeneration and cancer treatment. © 2025 The Royal Society of Chemistry.