Browsing by Author "Surya Prakash Dube"

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Advances in Trichoderma biology for biocontrol applications
(Elsevier, 2025) Amrita Saxena; Riddha Dey; Surya Prakash Dube; Richa Raghuwanshi
Lauded globally as a potent biopesticide and biofertilizer, Trichoderma can be the mainspring for the much needed “Greener Revolution” in the world required to restore food security for the ever-teeming global population. The uniqueness of Trichoderma that singles it out from other microbes is its several survival strategies. It owns a high capability of “rhizosphere competence,” a term aptly suggested for microbial growth along with plant roots. The fungus efficiently colonizes the plant roots, establishing along with the existing microbial communities. Reshaping the soil microbiome has also been a major mechanism of plant growth promotion by Trichoderma. Its evolving lifestyle has not only made it successful in establishing a beneficial cross-talk with the host plant toward providing tolerance against abiotic and biotic stresses but also in activating transcriptional memory in plants for future stress responses. The chief criteria that it selects for survival is antagonism or biocontrol. The competition for nutrients and space primarily steers such a kind of activity. Trichoderma secretes diverse volatile compounds including alcohols, aldehydes, ketones, ethylene, hydrogen cyanide, and monoterpenes, as well as non-volatile compounds including peptaibols and diketopiperazine-like gliotoxin and gliovirin which are known to exhibit antibiotic activity. Another important mechanism adopted by Trichoderma is mycoparasitism (penetration/infection) and the production of cell wall-degrading enzymes for successful inhibition of phytopathogenic fungi. The role of Trichoderma in sustainable agriculture is expanding with the unrevealing secondary metabolites, and it is commercialized. The generation of new bioformulations based on microbial metabolites and living consortia can be the future solutions for the increasing toxins in soil and underground water. © 2025 Elsevier Inc. All rights reserved.
Biotechnological approaches in mitigating climate variability and anthropogenic factors
(Nova Science Publishers, Inc., 2023) Riddha Dey; Surya Prakash Dube; Seema Devi; Richa Raghuwanshi
Rapid climate change and harsh anthropogenic activities have affected the ecosystem beyond resilience. Human-natural systems interference has brought about a large number of negative impacts across the continents, which includes the rise in the sea level, biodiversity loss, altered ecosystem; structure and function, and human livelihoods. Many species have got extinct and many are listed as endangered. To protect them and restore the balance of the environment, it is crucial to sensitize humans and encourage the applications of biotechnological tools in ecosystem restoration. The interference of biotechnology in agriculture has helped in developing climate-resilient crops. The techniques like genetic engineering, meta-omics, and CRISPR Cas9 are some of the promising conservation technologies that are commercially affordable, reproducible, and need to be implemented at the pilot level. This chapter may help in representing possible approaches to improve adaptive capacity as well as restoration of the ecosystem. It deals with various effects of anthropogenic activities and climate changes on different ecosystems like the Himalayan ecosystem, desert ecosystem, terrestrial ecosystem, and aquatic ecosystem. It also focuses on the biotechnological techniques which can be used to diminish biodiversity loss and help restore the ecological balance. © 2023 Nova Science Publishers, Inc.
Green Solid-State Synthesis of Antibacterial Binary Organic Material: Crystal Growth, Physicochemical Properties, Thermal Study, Antibacterial Activity, and Hirshfeld Surface Analysis
(Multidisciplinary Digital Publishing Institute (MDPI), 2025) Adarsh Rai; Sumit Chaudhary; Surya Prakash Dube; Szymon Bajda; Richa Raghuwanshi; Shiva Kant Mishra; Gaetano Palumbo; Ramanand Rai
The organic compounds 2-aminopyrimidine (AP) and 4-aminobenzoic acid (PABA) were selected for the synthesis of a compound by establishing the phase diagram and adopting the solid-state synthesis method. The phase diagram analysis suggested the formation of a novel intermolecular compound (IMC) at a 1:1 stoichiometric ratio of AP and PABA, along with two eutectics at 0.25 and 0.90 mole fractions of AP. FTIR and NMR spectroscopy were used for the structure elucidation of the intermolecular compound. The powder X-ray diffraction analysis revealed the novel nature of IMC (APPABA) and the mechanical mixture nature of eutectics. The sharp and single peak of the DSC curve suggested the melting and pure nature of the synthesized IMC. Various thermodynamic parameters of IMC and eutectics were studied. A single crystal of the IMC was grown from solution and its single-crystal X-ray diffraction analysis revealed that it crystallized in a monoclinic system with the P21/n space group. Hirshfeld surface analysis further validated the weak non-covalent interactions summarized through the single-crystal X-ray analysis. Studies on the IMC were thoroughly conducted to evaluate its antibacterial activity with reference to antibiotics, and it showed significant positive responses against various pathogenic microbial isolates (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella aerogenes, and Shigella boydii) and non-pathogenic microbial isolates (Enterobacter cloacae, Pseudomonas azotoformans, and Burkholderia paludis). It was also found effective against methicillin-resistant bacterial strains viz. Staphylococcus aureus MRSA. © 2025 by the authors.
The potential of Burkholderia sp. in meeting the goals of sustainable agriculture
(Nova Science Publishers, Inc., 2023) Richa Raghuwanshi; Seema Devi; Surya Prakash Dube
Interest in sustainable approaches to biological control of plant pathogens and plant-growth augmentation has been driven by environmental and public concerns. Burkholderia sp., a diverse and adaptable bacterial genus is an incredibly versatile Ggram-negative genus with over 120 species. Burkholderia, a phylogenetically coherent genus wellrecognized in agricultural applications are often isolated from infected patients as well. Recent studies have demonstrated that the pH of the soil has a significant impact on its biogeographic range. Burkholderia sp. exhibiting laccase activity is beneficial for the survival of the strain in phenol-rich environments. Burkholderia is an important bacterial species that directly promotes plant growth by phosphate solubilization, nitrogen fixation, phytohormone production and indirectly by producing antibiotics and siderophores, inhibiting phytopathogens in diverse crops. It is also well-known for its bioremediation, biopesticidal activities as well. The genus holds potential in producing multiple antibiotics by quorum sensing, a cell-density-dependent regulatory mechanism. The inhibitory metabolites produced by Burkholderia including pyrrolnitrin, phenylpyrroles, altericidin A, altericidin B, altericidin C, bacteriocins, and a novel lipopeptide are active against diverse organisms, including the plant parasitic nematode, bacteria, and several important soil-borne plant-pathogenic fungi. The goal of sustainable agriculture is to develop efficient, biological systems that don't need high levels of material inputs. Healthy soil is an important component of sustainability and Burkholderia sp. holds immense possibilities to enhance and protect the productivity of the soil. The multifaceted roles exhibited by Burkholderia sp. in attaining the sustainable agricultural practices are dealt in detail in the present chapter. © 2023 Nova Science Publishers, Inc. All rights reserved.