Browsing by Author "Swapnil C. Kamble"

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Assessment of Biological Activities of Fungal Endophytes Derived Bioactive Compounds Isolated from Amoora rohituka
(MDPI, 2022) Ashish Verma; Priyamvada Gupta; Nilesh Rai; Rajan Kumar Tiwari; Ajay Kumar; Prafull Salvi; Swapnil C. Kamble; Santosh Kumar Singh; Vibhav Gautam
Fungal endophytes have remarkable potential to produce bioactive compounds with numerous pharmacological significance that are used in various disease management and human welfare. In the current study, a total of eight fungal endophytes were isolated from the leaf tissue of Amoora rohituka, and out of which ethyl acetate (EA) extract of Penicillium oxalicum was found to exhibit potential antioxidant activity against DPPH, nitric oxide, superoxide anion and hydroxyl free radicals with EC50 values of 178.30 ± 1.446, 75.79 ± 0.692, 169.28 ± 0.402 and 126.12 ± 0.636 µg/mL, respectively. The significant antioxidant activity of EA extract of P. oxalicum is validated through highest phenolic and flavonoid content, and the presence of unique bioactive components observed through high-performance thin layer chromatography (HPTLC) fingerprinting. Moreover, EA extract of P. oxalicum also displayed substantial anti-proliferative activity with IC50 values of 56.81 ± 0.617, 37.24 ± 1.26 and 260.627 ± 5.415 µg/mL against three cancer cells HuT-78, MDA-MB-231 and MCF-7, respectively. Furthermore, comparative HPTLC fingerprint analysis and antioxidant activity of P. oxalicum revealed that fungal endophyte P. oxalicum produces bioactive compounds in a host-dependent manner. Therefore, the present study signifies that fungal endophyte P. oxalicum associated with the leaf of A. rohituka could be a potential source of bioactive compounds with antioxidant and anticancer activity. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Beyond the synthetic drugs: Fungal endophytes derived bioactive compounds in the management of neurodegenerative disorders
(Bentham Science Publishers, 2023) Ashish Verma; Nilesh Rai; Swapnil C. Kamble; Pradeep Mishra; Suvakanta Barik; Rajiv Kumar; Santosh Kumar Singh; Prafull Salvi; Vibhav Gautam
Fungal endophytes are a group of fungi that reside in plant tissues and show a symbiotic relationship with the host plants. They protect against pathogens and increase food availability without causing any harmful effects on the host plant. Fungal endophytes are known to produce a wide range of bioactive compounds with several biological activities, including neuroprotective effects. Neurodegenerative disorders lead to miscommunication between nerve cells, damage or loss in structure and function of the central nervous system (CNS) or peripheral nervous system (PNS). Reactive oxygen species, neuroinflammation, protein degradation or aggregation, familial history, mutation in mitochondrial genes, and aging contribute to neurodegenerative disorders. Plant-associated fungal endophytes produce bioactive compounds, which show anti-neuroinflammatory, antioxidant, and anti-cholinesterase activities. Several pro-inflammatory (TNF-α and NF-κB) and depressant (serotonin, dopamine, and noradrenaline) molecules or neuronal signaling pathways leading to neurodegenerative disorders are known to be inhibited or down-regulated by fungal endophyte-derived bioactive compounds. Therefore, bioactive compounds produced from fungal endophytes could be a promising approach to treating various health ailments. The present chapter discusses selected fungal endophyte-derived potential bioactive compounds with neuroprotective effects for managing neurodegenerative disorders. © 2023 Bentham Science Publishers. All rights reserved.
Biological potential of bioactive metabolites derived from fungal endophytes associated with medicinal plants
(Springer Science and Business Media Deutschland GmbH, 2021) Priyanka Kumari Keshri; Nilesh Rai; Ashish Verma; Swapnil C. Kamble; Suvakanta Barik; Pradeep Mishra; Santosh Kumar Singh; Prafull Salvi; Vibhav Gautam
Endophytes are endosymbiotic fungi or bacteria that invade and colonize the plant tissue without harming their respective host. Since fungal endophytes live inside the host tissues, their effective isolation and identification is an important step. Endophytic fungi produce bioactive metabolites with unique chemical structures, which may help in improving the physiological condition of both the plants and endophytes. Endophytic fungi are a potent source of all major classes of secondary metabolites including terpenoids, non-ribosomal peptides, polyketides, and alkaloids. With the increase in the number of diseases and human health problems, an intensive search for new sources and effective metabolites is now in progress. The bioactive metabolites produced by the fungal endophytes are competent and effective against various diseases such as cancer, diabetes, infectious diseases, immunological disorders, and cardiovascular diseases. The present review summarizes the efficient approaches used for the screening of fungal endophytes, extraction, and purification of bioactive metabolites along with OMICS based study of endophytes. Along with this, the pharmaceutical importance of novel bioactive metabolites and their efficient production from fungal endophytes using various approaches like genetic engineering, use of elicitors, and precursor feeding are also discussed. © 2021, German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature.
Bioprospecting of fungal endophytes from Oroxylum indicum (L.) Kurz with antioxidant and cytotoxic activity
(Public Library of Science, 2022) Nilesh Rai; Priyanka Kumari Keshri; Priyamvada Gupta; Ashish Verma; Swapnil C. Kamble; Santosh Kumar Singh; Vibhav Gautam
Oroxylum indicum (L.) Kurz, a medicinal plant, shows numerous pharmacological properties which may be attributed to the bioactive compounds produced by O. indicum or due to associated endophytes. In the present study, leaf of O. indicum was evaluated for the presence of associated fungal endophytes, and antioxidant and cytotoxic activities of bioactive compounds produced from them. Using culture-dependent approach, eight fungal endophytes belonging to five different genera were identified. Two endophytes Daldinia eschscholtzii and Ectophoma multirostrata have been reported for the first time from the leaf of O. indicum plant. High-performance thin-layer chromatography (HPTLC) of ethyl acetate (EA) extract of isolated fungal endophytes showed a distinct fingerprinting profile in EA extract of Colletotrichum gloeosporioides. Among identified endophytes, EA extract of C. gloeosporioides showed significant antioxidant activity against DPPH free radical, superoxide anion radical, nitric oxide radical and hydroxyl radical with EC50 values of 22.24±1.302 μg/mL, 67.46 ±0.576 μg/mL, 80.10±0.706 μg/mL and 61.55±1.360 μg/mL, respectively. EA extract of C. gloeosporioides exhibited potential cytotoxicity against HCT116, HeLa and HepG2 cancer cell lines with IC50 values of 76.59 μg/mL, 176.20 μg/mL and 1750.70 μg/mL, respectively. A comparative HPTLC fingerprinting and the antioxidant activity of C. gloeosporioides associated with two different hosts (leaf of O. indicum and dead twigs of other plant) showed that C. gloeosporioides produces bioactive compounds in a host-dependent manner. Copyright: © 2022 Rai et al.
Ethyl Acetate Extract of Colletotrichum gloeosporioides Promotes Cytotoxicity and Apoptosis in Human Breast Cancer Cells
(American Chemical Society, 2023) Nilesh Rai; Priyamvada Gupta; Ashish Verma; Rajan Kumar Tiwari; Prasoon Madhukar; Swapnil C. Kamble; Ajay Kumar; Rajiv Kumar; Santosh Kumar Singh; Vibhav Gautam
Fungal endophytes are known to be a paragon for producing bioactive compounds with a variety of pharmacological importance. The current study aims to elucidate the molecular alterations induced by the bioactive compounds produced by the fungal endophyte Colletotrichum gloeosporioides in the tumor microenvironment of human breast cancer cells. GC/MS analysis of the ethyl acetate (EA) extract of C. gloeosporioides revealed the presence of bioactive compounds with anticancer activity. The EA extract of C. gloeosporioides exerted potential plasmid DNA protective activity against hydroxyl radicals of Fenton’s reagent. The cytotoxic activity further revealed that MDA-MB-231 cells exhibit more sensitivity toward the EA extract of C. gloeosporioides as compared to MCF-7 cells, whereas non-toxic to non-cancerous HEK293T cells. Furthermore, the anticancer activity demonstrated by the EA extract of C. gloeosporioides was studied by assessing nuclear morphometric analysis and induction of apoptosis in MDA-MB-231 and MCF-7 cells. The EA extract of C. gloeosporioides causes the alteration in cellular and nuclear morphologies, chromatin condensation, long-term colony inhibition, and inhibition of cell migration and proliferation ability of MDA-MB-231 and MCF-7 cells. The study also revealed that the EA extract of C. gloeosporioides treated cells undergoes apoptosis by increased production of reactive oxygen species and significant deficit in mitochondrial membrane potential. Our study also showed that the EA extract of C. gloeosporioides causes upregulation of pro-apoptotic (BAX, PARP, CASPASE-8, and FADD), cell cycle arrest (P21), and tumor suppressor (P53) related genes. Additionally, the downregulation of antiapoptotic genes (BCL-2 and SURVIVIN) and increased Caspase-3 activity suggest the induction of apoptosis in the EA extract of C. gloeosporioides treated MDA-MB-231 and MCF-7 cells. Overall, our findings suggest that the bioactive compounds present in the EA extract of C. gloeosporioides promotes apoptosis by altering the genes related to the extrinsic as well as the intrinsic pathway. Further in vivo study in breast cancer models is required to validate the in vitro observations. © 2023 The Authors. Published by American Chemical Society.
Nanoparticulate curcumin spray imparts prophylactic and therapeutic properties against SARS-CoV-2
(Springer Nature, 2024) Swapnil C. Kamble; Prem Pandey; Vijay K. Kanuru; Nilesh Rai; Vibhav Gautam; Dinesh Amalnerkar
Prevention of transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the key to limit spread of COVID-19. Pharmaceutical preparation of curcumin, a natural polyphenolic compound, has been proven to have anti-inflammatory action. To have accomplishable function, nanosized curcumin may dictate a functionality against SARS-CoV-2. Herein, we used a unique water-dispersible nano-spray formulation of curcumin with enhanced efficacy. The nano-curcumin has an average size of 20 nm. No cytotoxicity was detected at the tested concentrations on Vero E6 cells, which is consistent with curcumin’s GRAS status of FDA. Vero E6 cell-assays demonstrated effective inhibitory activity when pre-treated with curcumin nanoformulation. Therapeutic effect on infected cells by subsequent exposure to SARS-CoV-2 viral particles shows considerable control on viral particle spread. Given the safety and efficacy of nano-curcumin, it appears to be an ideal candidate for prophylactic protection as well as in reducing the viral transmission. © Qatar University and Springer Nature Switzerland AG 2024.
Natural compound-based scaffold to design in vitro disease systems
(Elsevier, 2023) Chirag Varshney; Brijesh Kumar; Swapnil C. Kamble
Traditional disease evaluation models of animal systems and two-dimensional (2D) systems are extremes of executability and reflection of true disease state. By using the in vitro three-dimensional scaffold, a sustainable path could be achieved that is similar to disease conditions without the use of animals. Scaffold-based three-dimensional (3D) systems can replicate the natural physical architecture as well as are able to induce similar genotypic and phenotypic gene expressions in the cells. Scaffolds can be constructed either by artificial or natural sources. However, in many cases, natural scaffolds show the best result in term of cell proliferation and differentiation with extracellular matrix cell secretion properties. Because of their natural origin, such scaffolds lack mechanical strength and tunability which can be easily overcome by mixing them with other biocompatible compounds to make hydrogel. In this chapter, we discuss the significance of natural compounds for the preparation of scaffolds and their implication for the disease model. © 2023 Elsevier Inc. All rights reserved.
Plant associated fungal endophytes as a source of natural bioactive compounds
(Taylor and Francis Ltd., 2021) Nilesh Rai; Priyanka Kumari Keshri; Ashish Verma; Swapnil C. Kamble; Pradeep Mishra; Suvakanta Barik; Santosh Kumar Singh; Vibhav Gautam
Endophytes are a potent source of bioactive compounds that mimic plant-based metabolites. The relationship of host plant and endophyte is significantly associated with alteration in fungal colonisation and the extraction of endophyte-derived bioactive compounds. Screening of fungal endophytes and their relationship with host plants is essential for the isolation of bioactive compounds. Numerous bioactive compounds with antioxidant, antimicrobial, anticancer, and immunomodulatory properties are known to be derived from fungal endophytes. Bioinformatics tools along with the latest techniques such as metabolomics, next-generation sequencing, and metagenomics multilocus sequence typing can potentially fill the gaps in fungal endophyte research. The current review article focuses on bioactive compounds derived from plant-associated fungal endophytes and their pharmacological importance. We conclude with the challenges and opportunities in the research area of fungal endophytes. © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
Preface
(Springer Science and Business Media B.V., 2024) Vibhav Gautam; Rajiv Kumar; Krishna Das Manandhar; Swapnil C. Kamble
[No abstract available]
Probiotics for Management of Gastrointestinal Cancers
(Springer Singapore, 2020) Nilesh Rai; Anurag Kumar Singh; Priyanka Kumari Keshri; Suvakanta Barik; Swapnil C. Kamble; Santosh Kumar Singh; Rajiv Kumar; Pradeep Mishra; Deepak Kotiya; Vibhav Gautam
Probiotics has been used for management of genetically predisposed health disorders associated with gastrointestinal (GI) tract. Administration of specific probiotics in defined doses causes revitalization of healthy gut microflora that can positively modulate immune response within GI tract and hence, aid in the management of inflammation of intestinal mucosa. Thus, it has potential of becoming a durable therapeutic approach to resolve metabolism related disorders including GI cancers. Probiotic-induced competition can exclude and replace pathogenic microorganisms from GI cancer-induced niche in gut. Notably, oral administration of probiotics is a key driving factor for the ease of management of post-operative complications of GI tract cancers. Here, we attempt to summarize the diversified knowledge of probiotics to utilize as therapeutic tool in prevention, progression and treatment of GI tract cancer, current challenges of probiotics in regulating GI cancer progression, and future perspectives. © Springer Nature Singapore Pte Ltd. 2021, corrected publication 2021.
Recent technological advancements in stem cell research for targeted therapeutics
(Springer, 2020) Nilesh Rai; Anurag Kumar Singh; Santosh Kumar Singh; Bhaskar Gaurishankar; Swapnil C. Kamble; Pradeep Mishra; Deepak Kotiya; Suvakanta Barik; Neelam Atri; Vibhav Gautam
Stem cells have characteristic features of self-renewal, pluripotency and differentiation, which are responsible for replenishment of tissue or organ. Stem cells are potentiated as therapeutic tool in drug targeting and regenerative medicine—from curing various neurological diseases and malignancies to congenital diseases. These technological advancements have established stem cells as future of medicine. However, due to ethico-social limitations, the use of embryonic stem cells (ESCs) has been avoided, while physiological availability of adult stem cells (ASCs) and induced pluripotent stem cells (iPSCs) has gained appropriate preference. These iPSCs are very much similar to ESCs in terms of their self-renewal and pluripotency. Here, we have summarized the technologies that have established stem cells isolation, their molecular marker and factors responsible for their maintenance. Different cellular (transcription factors, regulatory proteins, miRNA like miRNA-296, miRNA-145, etc.) and extracellular components transcend stem cell fate. Their identification and characterization involve development and efficient utilization of tools like magnetic activated cell sorting (MACS) and fluorescence activated cell sorting (FACS). Some of the technologies have been patented and spin-off’s based on them have been commercialized. In conclusion, we present the future scope and possibilities that stem cell technologies behold for us. [Figure not available: see fulltext.]. © 2020, Controlled Release Society.
Unveiling the cytotoxic and anti-proliferative potential of green-synthesized silver nanoparticles mediated by Colletotrichum gloeosporioides
(Royal Society of Chemistry, 2024) Priyamvada Gupta; Swati Singh; Nilesh Rai; Ashish Verma; Harshita Tiwari; Swapnil C. Kamble; Hemant Kumar Gautam; Vibhav Gautam
Fungal endophytes are a putative source of bioactive metabolites that have found significant applications in nanomedicine due to their metabolic versatility. In the present study, an aqueous extract of the fungal endophyte, Colletotrichum gloeosporioides associated with a medicinal plant Oroxylum indicum, has been used for the fabrication of green silver nanoparticles (CgAgNPs) and further evaluated their cytotoxic and anti-proliferative activity. Bioanalytical techniques including UV-Vis spectral analysis revealed a sharp band at 435 nm and functional molecules from the aqueous extract involved in the synthesis of CgAgNPs were evidenced through FTIR. Further, the crystalline nature of CgAgNPs was determined through XRD analysis and microscopy techniques including AFM, TEM and FESEM demonstrated the spherical shape of CgAgNPs exhibiting a crystalline hexagonal lattice and the size was found to be in the range of 9-29 nm. The significant cytotoxic potential of CgAgNPs was observed against breast cancer cells, MDA-MB-231 and MCF-7 with IC50 values of 18.398 ± 0.376 and 38.587 ± 1.828 μg mL−1, respectively. The biochemical study revealed that the treatment of MDA-MB-231 and MCF-7 cells with CgAgNPs reduces glucose uptake, suppresses cell proliferation, and enhances LDH release, indicating reduced cell viability and progression. Moreover, our research revealed differential expression of genes associated with apoptosis, cell cycle inhibition and metastasis suppression, evidencing anti-proliferative activity of CgAgNPs. The main objective of the present study is to harness anti-breast cancer activity of novel biogenic nanoparticles synthesized using the aqueous extract of O. indicum associated C. gloeosporioides and study the underlying mechanistic pathway exerted by these mycogenic nanoparticles. © 2024 The Royal Society of Chemistry.