Browsing by Author "Tanmay Kumar Koley"

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Antioxidant Potentiality and Mineral Content of Summer Season Leafy Greens: Comparison at Mature and Microgreen Stages Using Chemometric
(Springer, 2019) Lalu Prasad Yadav; Tanmay Kumar Koley; Ajay Tripathi; Surendra Singh
Nine summer season leafy greens viz., Amaranthus, bottle gourd, cucumber, jute, palak, poi, pumpkin, radish, water spinach, were evaluated for their antioxidants and mineral content at microgreen and mature stages. Among the antioxidants, total phenolics, total flavonoids, and ascorbic acid were quantified. Besides, antioxidant activities of the leafy vegetables were also measured using four in vitro assays viz., ferric reducing antioxidant power (FRAP), cupric reducing antioxidant power (CUPRAC), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and Trolox equivalent antioxidant capacity assays (TEAC). In addition to this, the content of selected elements such as potassium (K), iron (Fe), manganese (Mn), zinc (Zn), and copper (Cu) was estimated. A wide variation was observed in the content of antioxidants and minerals. Variation was also observed for cultivar to cultivar as in case of Amaranthus. Results showed that the total phenolic content varied from 95.73 to 313.92 mg Gallic acid equivalent (GAE)/100 g in the mature vegetables, whereas the value varied from 25.00 to 152.10 mg GAE/100 g in microgreens. In fact, mature leafy vegetables were found to be significantly higher sources for total phenolics than microgreens. Likewise, a similar trend was observed for total flavonoids content and antioxidant activities. On the contrary, in all the species the concentration of K and Zn was significantly higher in microgreens than mature vegetables. However, no specific trend was observed in case of Fe, Cu, and Mn content. Based on antioxidant potentiality and mineral content, these leafy greens formed three distinct clusters; the first cluster represented by Amaranthus cv Local Green, jute, bottle gourd, and water spinach at mature stages. Jute was found to be the best, followed by bottle gourd, Amaranthus cv. Local Green, and water spinach. © 2018, NAAS (National Academy of Agricultural Sciences).
Coupling the high-resolution LC–MS characterisation of the phenolic compounds with the antimicrobial and antibiofilm properties of helencha (Enydra fluctuans Lour.)
(Springer, 2021) Tanmay Kumar Koley; Zareen Khan; Dasharath Oulkar; Tuhina Banerjee; Aradhana Singh; Suhas Gorakh Karkute; Kaushik Banerjee
This study reports the polyphenol profile of helencha (Enydra fluctuans Lour.), an underutilised, aquatic leafy vegetable, based on high resolution accurate mass analysis. The methanolic extract of helencha leaves was screened by ultra-high performance liquid chromatography with quadrupole time of flight mass spectrometry (LC-QToF-MS). An in-house developed database of phytochemical metabolites was referred for compound identifications. Based on the detection of the pseudomolecular ion and at least one molecule-specific fragment ion (each with < 5 ppm of mass error), 25 potentially-bioactive phenolic compounds were putatively identified. These included 6 flavonols, 4 phenolic acids, 3 lignans, 3 flavones and 1 each of flavanol, flavanone, dihydroflavonol, tetramethoxyflavone, isoflavonoid and methylated flavonol. In addition, 3 unclassified compounds are also reported. The helencha extract showed antibiofilm properties with a potent bacteriostatic activity against the clinical isolates of Pseudomonas aeruginosa, a human pathogenic bacteria. The complementary molecular docking studies indicated strong binding interactions of the identified compounds with the active site of LasR protein of P. aeruginosa. The in vitro and in silico study results would be useful to develop novel neutraceutical products based on helencha-extract and design new lead compounds to control the biofilm producing pathogenic microorganisms. © 2021, Association of Food Scientists & Technologists (India).
High-Resolution LCMS Profiling of Phenolic Compounds of Indian Black Carrot and Evaluation of Its Effect on Antioxidant Defense and Glucose Metabolism in Animal Model
(Springer, 2019) Tanmay Kumar Koley; Shivani Srivastava; Y.B. Tripathi; Kaushik Banerjee; Dasharath Oulkar; Arnab Goon; Ajay Tripathi; Bijendra Singh
Black carrot (Daucus carota L. ssp. sativus var. atrorubens Alef.), an indigenous vegetable of India, has been widely used for the preparation of traditional fermented product Kanji, and traditional medicine for treating various kinds of diseases. Although previous research has reported its biochemical composition and some in vitro functional properties, very scanty information is available to date on the in vivo effect of black carrot. Given this gap of knowledge, this study was aimed to investigate the effect of black carrot on antioxidant defense, glucose metabolism and renal, as well as hepatic toxicity in normal Charles Foster albino rats. In addition, secondary metabolites were identified by high-resolution LCMS and in vitro antioxidant activity was also assessed. LC/MS/MS analysis revealed the presence of nine anthocyanins and two flavonols. Among these, four new compounds were putatively identified for the first time. Under in vivo condition, feeding of black carrot juice for 30 days resulted in the reduction in the superoxide dismutase activity, both in dose- and time-dependent manner. It was also observed that feeding of rats with black carrot juice for 30 days reduced their blood triglyceride concentration, both in dose- and time-dependent manners. No significant effect was observed on plasma glucose concentration, and also, no toxicity was noted with regard to the functioning of the kidney and liver. © 2018, NAAS (National Academy of Agricultural Sciences).
Ovicidal and larvicidal effects of extracts from leaves of Andrographis paniculata (Burm. f.) Wall.ex Nees against field isolates of human hookworm (Ancylostoma duodenale)
(Elsevier Ireland Ltd, 2019) Tuhina Banerjee; Aradhana Singh; Satyanshu Kumar; Tushar Dhanani; N.A. Gajbhiye; Tanmay Kumar Koley; Arti Maurya; Joel Filgona
Ethnopharmacological relevance: The whole plant of Andrographis paniculata (Burm. f.) Wall.ex Nees is used traditionally in different forms by the local people of Asian countries owing to its myriad medicinal properties. Its use as an anthelmintic has been mentioned in literature but has not been well elucidated. Aim of the study: To determine anthelmintic effects of extracts from leaves of A.paniculata against human hookworm species based on a standard assay system and to establish the effects of major active compounds responsible for the effects. Materials and methods: Ovicidal and larvicidal activities of extracts of leaves of A.paniculata in different solvents ethanol (Et), methanol (Met), ethyl acetate (EA) and petroleum ether (PE) was studied against field isolates of Ancylostoma duodenale collected and cultivated from hookworm infected human stool samples by egg hatch and larval motility assays. Major active compounds namely andrographolide (AP1), neoandrographolide (AP2) and andrograpanin (AP3) were estimated quantitatively in all the extracts by high-performance liquid chromatography (HPLC) and mass spectrometry (MS) analysis. Anthelmintic effects (ED 50 , LC 50 ) and presence of the marker compounds in each extract was statistically analyzed by principal component analysis (PCA). Further, biological activities of pure compounds of AP1, AP2, AP3 were assessed to validate the results of the study. Results: Extracts in ethanol and methanol showed highest activity in inhibition of egg hatching with lowest ED 50 values (0.017 and 0.02 mg/mL respectively) while ethyl acetate extract had the highest activity against larval motility (0.001 mg/mL) followed by ethanol (0.019 mg/mL). On HPLC analysis, andrographolide content (%), the major diterpene compound, in Met and Et was 0.85 and 1.43 respectively. On PCA, andrographolide component in the extracts was associated with significant inhibitory effects both on egg hatching and larval motility. Pure compound AP1 also showed significant ovicidal and larvicidal activities at concentrations 0.125 µg/mL and 0.019 mg/mL respectively. Conclusion: Andrographolide is one of the main phytochemical responsible for significant ovicidal and larvicidal activity against field isolates of A.duodenale from human infections and can be developed as a potential therapeutic choice. © 2019 Elsevier B.V.
The tuber extract of pueraria tuberosa Linn. competitively inhibits DPP-IV activity in normoglycemic rats
(International Journal of Pharmacy and Pharmaceutical Science, 2015) Shivani Srivastava; Tanmay Kumar Koley; Surya Kumar Singh; Yamini Bhusan Tripathi
Objective: The main objective of this research was to explore the effect of the polar fraction of tubers of Pueraria tuberosa (PTWE) on DPP-IV activity. Methods: The comparison of in vitro inhibitory potential between commercially available Galvus (Vildagliptin) and PTWE was determined by measuring percent inhibition and IC50. The enzyme kinetics were also done to reveal the nature of inhibition. In vivo study was done via the glucose tolerance test and by the measurement of increased plasma GLP-1 concentration and DPP-IV activity after glucose load. Results: PTWE has given the IC50 value of 17.4 mg/ml and was found to be a competitive inhibitor having the Ki value of 13.11 mg/ml. Plasma GLP-1 concentration was increased and DPP-IV activity was decreased after 60 minutes of glucose load in PTWE treated rats as compared to control rats. Overall PTWE was found to be less potential DPP-IV inhibitor than Galvus. Conclusion: These findings suggest that antidiabetic effect of PTWE could be because of its role in DPP-IV inhibition. © 2015, International Journal of Pharmacy and Pharmaceutical Science. All rights reserved.