Browsing by Author "Tarun K. Banerjee"

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Biochemical Alteration in the Labeo rohita Tissues Following Exposure to the Effluent Generated during Extraction of Metals from Polymetallic Sea Nodules
(Wiley-VCH Verlag, 2014) Huma Vaseem; Tarun K. Banerjee
Effect of the effluent generated during extraction of precious metals from polymetallic sea nodules have been illustrated by analyzing the biochemical alterations in freshwater fish Labeo rohita. Increase or decrease in the different biomolecules in various tissues (skin, muscles, gills, liver, kidney, and brain) observed was discussed in the light of stress caused by exposures of this effluent. Decrease in concentration of glycogen, total lipids, cholesterol, and total protein in most of the tissues indicate their role in providing the continuous energy requirement under toxic stress in the exposed fish. Reduced DNA and RNA concentration in exposed fish showed effluent toxic effect on DNA synthesis and transcriptional level. With the prolongation of exposure, reduction in activities of aspartate amino transferase, alanine amino transferase, and alkaline phosphatase were observed in all the exposed tissues indicating their cellular degeneration. In conclusion, the sea nodule effluent was found to be very toxic and have negative health impact on the fish. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Changes in the level of proteins, free amino acids and protease activities of clarias batrachus in response to sodium meta-arsenite intoxication
(2013) Randhir Kumar; Tarun K. Banerjee
Increased hazard of arsenic toxicity at many provinces of India causing extensive contamination of their wetlands. To illustrate the consequences of the arsenic menace on the aquatic fauna, a nutritionally important freshwater catfish Clarias batrachus (Linn.) was subjected to the stress of sublethal concentration (1mg/L; 5% of 96-h LC50 value) of sodium meta-arsenite. The alterations induced by the arsenic salt on certain biomolecules (proteins, free amino acids (FAA), and protease activities) in five tissue components (muscle, liver, brain skin, and gills) of the fish using biochemical techniques were estimated. Significant (p<0.01) decrease in the concentration of proteins in all the tissues (19.74-44.84% in muscles; 3.17-49.39% in liver; 10.05-36.16% in brain; 12.62-52.01% in skin and 7.34-61.69% in gills), and significant (p<0.01) increase in FAA (6.84-269.89% in muscles; 125.89-358.03% in liver; 1.07-152.90% in brain; 29.31-401.62% in skin and 38.53-165.07% in gills), and protease activities (43.59-183.33% in muscles; 19.52-131.90% in liver; 8.85-46.72% in brain; 33.33-261.53% in skin and 25.39-50.79% in gills) of the exposed fish were observed. This increase in amino acids and enzyme activity point out toward breakdown of protein leading to their depletion. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Study of sodium arsenite induced biochemical changes on certain biomolecules of the freshwater catfish Clarias batrachus
(2012) Randhir Kumar; Tarun K. Banerjee
Toxic impact of sublethal concentration (1 mg/L; 5% of 96h LC50 value) of sodium arsenite (NaAsO2) on certain biomolecules (proteins, nucleic acids, lipids, and glycogen) of five tissue components (muscles, liver, brain, skin, and gills) of the freshwater catfish Clarias batrachus was analysed. The important toxic manifestations include marked decrease in the concentration of proteins (21.72-45.42% in muscles; 3.42-53.94% in liver; 15.39-45.42% in brain; 15.40-4.00% in skin and 11.35-64.13% in gills), DNA (0.55-22.95% in muscles; 8.33-14.06% in liver; 5.30-18.40% in brain; 13.57-52.80% in skin; and 12.38-31.01% in gills), RNA (42.68-76.16% in muscles; 10.68-39.75% in liver; 5.66-29.05% in brain; 7.72-27.93% in skin and 21.47-44.38% in gills) and glycogen (24.00-51.72% in muscles; 49.11-72.45% in liver; 11.49-26.03% in brain; 26.13-38.05% in skin and 17.80-37.97% in gills). Excepting liver where the lipid content increases (15.82-24.13%), the fat content also showed depletion in their concentration (10.40-29.83% in muscles; 8.30-34.45% in brain; 8.94-31.47% in skin and 12.75-28.86% in gills), in the rest of the organ systems. © 2012 Sociedade Brasileira de Ictiologia.