Browsing by Author "Tatiana Minkina"

Now showing 1 - 20 of 55

A Dual Therapeutic Approach to Diabetes Mellitus via Bioactive Phytochemicals Found in a Poly Herbal Extract by Restoration of Favorable Gut Flora and Related Short-Chain Fatty Acids
(Springer, 2024) Amit Kumar Singh; Pradeep Kumar; Sunil Kumar Mishra; Vishnu D. Rajput; Kavindra Nath Tiwari; Anand Kumar Singh; Tatiana Minkina; Ajay Kumar Pandey; Prabhat Upadhyay
Diabetes mellitus (DM), a metabolic and endocrine condition, poses a serious threat to human health and longevity. The emerging role of gut microbiome associated with bioactive compounds has recently created a new hope for DM treatment. UHPLC-HRMS methods were used to identify these compounds in a poly herbal ethanolic extract (PHE). The effects of PHE on body weight (BW), fasting blood glucose (FBG) level, gut microbiota, fecal short-chain fatty acids (SCFAs) production, and the correlation between DM-related indices and gut microbes, in rats were investigated. Chebulic acid (0.368%), gallic acid (0.469%), andrographolide (1.304%), berberine (6.442%), and numerous polysaccharides were the most representative constituents in PHE. A more significant BW gain and a reduction in FBG level towards normal of PHE 600 mg/kg treated rats group were resulted at the end of 28th days of the study. Moreover, the composition of the gut microbiota corroborated the study’s hypothesis, as evidenced by an increased ratio of Bacteroidetes to Firmicutes and some beneficial microbial species, including Prevotella copri and Lactobacillus hamster. The relative abundance of Bifidobacterium pseudolongum, Ruminococcus bromii, and Blautia producta was found to decline in PHE treatment groups as compared to diabetic group. The abundance of beneficial bacteria in PHE 600 mg/kg treatment group was concurrently associated with increased SCFAs concentrations of acetate and propionate (7.26 nmol/g and 4.13 nmol/g). The findings of this study suggest a promising approach to prevent DM by demonstrating that these naturally occurring compounds decreased FBG levels by increasing SCFAs content and SCFAs producing gut microbiota. Graphical Abstract: Flow chart summarizing research on the dual therapeutic approach to diabetes mellitus via bioactive chemicals found in a poly herbal extract and the management of gut microbiota in relation to DM. (SCFAs, short chain fatty acids; SMB53, a genus of bacterial microbiota of small intestine; LPS, lipopolysaccharide) (Figure presented.) © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024.
A review on phytotoxicity and defense mechanism of silver nanoparticles (AgNPs) on plants
(Springer Science and Business Media B.V., 2023) Sumit Kumar; Prahlad Masurkar; Bana Sravani; Dipanjali Bag; Kamal Ravi Sharma; Prashant Singh; Tulasi Korra; Mukesh Meena; Prashant Swapnil; Vishnu D. Rajput; Tatiana Minkina
Silver nanoparticles (AgNPs) are noteworthy used nanomaterials in a wide array of fields, particularly in the agricultural sector. Plants play a multifarious role in the ecosystem and provide a source of food for mankind. The responsibility of the scientific community is to recognize the deleterious impact of AgNPs (1–100 nm in size) on critical crop growth and development of plants, which is required for the assessment of environmental threats to plant, human, and animal health. The continued use of AgNPs in agriculture areas may have negative effects on plant biochemical and physiological responses. The current context focused mainly on AgNPs uptake, transport, and accumulation on crop plants and summarizes different levels of phytotoxicity of AgNPs on plant functions and focused on mechanisms of phytotoxicity employed by AgNPs. Moreover, some tolerance mechanisms and various survival strategies developed by plants under AgNPs toxicity are discussed. This background provides comprehensive information necessary to facilitate profound understanding of the toxic impacts of AgNPs on crop plants. © 2023, The Author(s), under exclusive licence to Springer Nature B.V.
Appraisal on accumulation of nanoenabled agrochemicals in plants with subsequent morphophysiological implications
(Elsevier, 2024) Pradeep Kumar; Kajal Singh; Amit Kumar Singh; Nancy Singh; Sakshi Singh; Vishnu D. Rajput; Tatiana Minkina; Sunil Kumar Mishra; Kavindra Nath Tiwari
The use of agricultural goods that are nanoenabled with nanotechnology, including nanoemulsions, nanoherbicides, nanofertilizers, and nanopesticides, to enhance the efficacy of agrochemical distribution to crop plants has become a more practical option. Many experiments have shown that the use of nanoagrochemicals has the efficacy of lowering the negative effects of chemical-derived fertilizer on the natural environment, in addition to significantly boosting crop yield. Still, new evidence suggests that goods made using nanotechnology not only have the ability to boost agricultural productivity but also bring about changes to the condition of the crop. There have been reports of variations in the amount of carbohydrates, amino acids, and starch present, in addition to the necessary metals. The levels of verbi gratia, albumin, globulin, and prolamin have dramatically increased in rice that has been subjected to CeO2-engineered nanoparticles (ENPs), whereas the levels of calcium, magnesium, and phosphorus have risen in different crops that received treatments treated with CeO2, CuO, and ZnO ENPs. On the other hand, researchers found that Mo and Ni levels dropped in both cucumbers and kidney beans after they were treated with synthetic nanoparticles made of CeO2 and ZnO, respectively. However, brief studies on the particular effects of nanoenabled agrochemical in agricultural area have been disscussed. © 2024 Elsevier Inc. All rights reserved.
Arsenic remediation through sustainable phytoremediation approaches
(MDPI, 2021) Sudhakar Srivastava; Anurakti Shukla; Vishnu D. Rajput; Kundan Kumar; Tatiana Minkina; Saglara Mandzhieva; Antonina Shmaraeva; Penna Suprasanna
Arsenic contamination of the environment is a serious problem threatening the health of millions of people exposed to arsenic (As) via drinking water and crops grown in contaminated areas. The remediation of As-contaminated soil and water bodies needs to be sustainable, low-cost and feasible to apply in the most affected low-to-middle income countries, like India and Bangladesh. Phytoremediation is an aesthetically appreciable and successful approach that can be used for As decontamination with use of the best approach(es) and the most promising plant(s). However, phytoremediation lacks the required speed and sometimes the stress caused by As could diminish plants’ potential for remediation. To tackle these demerits, we need augment plants’ potential with appropriate technological methods including microbial and nanoparticles applications and genetic modification of plants to alleviate the As stress and enhance As accumulation in phytoremediator plants. The present review discusses the As phytoremediation prospects of soil and water bodies and the usefulness of various plant systems in terms of high biomass, high As accumulation, bioenergy potential, and economic utility. The potential and prospects of assisted phytoremediation approaches are also presented. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Assessment of Heavy Metal Distribution and Health Risk of Vegetable Crops Grown on Soils Amended with Municipal Solid Waste Compost for Sustainable Urban Agriculture
(MDPI, 2023) Pallavi Bhardwaj; Rajesh Kumar Sharma; Abhishek Chauhan; Anuj Ranjan; Vishnu D. Rajput; Tatiana Minkina; Saglara S. Mandzhieva; Usha Mina; Shikha Wadhwa; Prakash Bobde; Ashutosh Tripathi
Rapid urbanization is one of the key factors that leads to defragmentation and the shrinking of agricultural land. It further leads to the generation of an ample amount of municipal waste. Several technologies have emerged in the past for its utilization, and in this regard, composting is one of the conventional approaches gaining popularity in modern agriculture. To overcome the possible criticality of intense urbanization, the concept of urban agriculture is taking shape. Municipal solid waste compost (MSWC) has been popularly explored for the soil amendments and nutritional requirements of crops. With this, the assessment of soil pollution (due to the heavy metals presently found in MSWC) is a required step for its safe application in agriculture. The present study aims at assessing the utilization of MSWC (in different ratios) to amend the soil and its impact on the growth and yield of brinjal (Solanum melongena), tomato (Solanum lycopersicum), and okra (Abelmoschus esculentus). The study also explored the uptake of heavy metals by plants and their risk to human consumption. The findings suggested that MSWC amendments upgraded the physio-chemical properties of soil, including organic matter (OM) and micronutrients, and increased the heavy metal concentrations in soil. Heavy metal analysis underlined the presence of several heavy metals both in soil and crops. Total metal concentration in soil increased with increased MSWC dosage. Concerning metal uptake by crop plants, 25% of MSWC was found to impart metal concentrations within permissible values in edible parts of crops. On the contrary, 50%, 75%, and 100% compost showed higher metal concentrations in the crops. A Health Risk Index (HRI) of less than 1 was found to be associated with soil amended with 25% MSWC. Our study implies that MSWC significantly improved the growth and yield of crops, and it can be considered an alternative to chemical fertilizer but only in a safer ratio (≤25%). However, further studies are required, especially on field conditions to validate the findings regarding metal accumulation. © 2023 by the authors.
Bacillus spp. as Bio-factories for Antifungal Secondary Metabolites: Innovation Beyond Whole Organism Formulations
(Springer, 2023) Bruno Salazar; Aurelio Ortiz; Chetan Keswani; Tatiana Minkina; Saglara Mandzhieva; Satyendra Pratap Singh; Bhagwan Rekadwad; Rainer Borriss; Akansha Jain; Harikesh B. Singh; Estibaliz Sansinenea
Several fungi act as parasites for crops causing huge annual crop losses at both pre- and post-harvest stages. For years, chemical fungicides were the solution; however, their wide use has caused environmental contamination and human health problems. For this reason, the use of biofungicides has been in practice as a green solution against fungal phytopathogens. In the context of a more sustainable agriculture, microbial biofungicides have the largest share among the commercial biocontrol products that are available in the market. Precisely, the genus Bacillus has been largely studied for the management of plant pathogenic fungi because they offer a chemically diverse arsenal of antifungal secondary metabolites, which have spawned a heightened industrial engrossment of it as a biopesticide. In this sense, it is indispensable to know the wide arsenal that Bacillus genus has to apply these products for sustainable agriculture. Having this idea in our minds, in this review, secondary metabolites from Bacillus having antifungal activity are chemically and structurally described giving details of their action against several phytopathogens. Knowing the current status of Bacillus secreted antifungals is the base for the goal to apply these in agriculture and it is addressed in depth in the second part of this review. © 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Bacillus subtilis-Mediated Induction of Disease Resistance and Promotion of Plant Growth of Vegetable Crops
(Springer, 2024) Sumit Kumar; Anjali; R. Arutselvan; Prahlad Masurkar; Udai B. Singh; Ruchi Tripathi; Ingudam Bhupenchandra; Tatiana Minkina; Chetan Keswani
Vegetable crops are the major nutrient source of food worldwide and are considered as perishable crops compared to cereal, oilseed, and legume crops. They play a crucial role in daily human life because they contain a plethora of immunity-boosting compounds like vitamins, proteins, carbohydrates, and crucial macro- and micronutrients important for human life. India contributes 16% global vegetable crop production, making it as second-largest producer globally. Vegetable crop production is significantly limited because, during their whole lives, they are attacked by an armada of noxious pathogens that reduce quality and quantity as well as suppress the current food supply. Synthetic chemicals are frequently used, which has detrimental impacts on macro- and microflora as well as the environment and human wellbeing. A continuous increment in the population of resistant pathogens to chemicals puts pressure on pathologists to investigate novel, sustainable, and best alternative methods to combat dangerous microbes. From the various kinds of plant disease management prospects, the implication of plant growth-promoting rhizobacteria, i.e. PGPRs, is becoming an effective substitute strategy worldwide because of their environmentally friendly nature. One of the most promising PGPRs representing sustainable agriculture growth is Bacillus subtilis, which has been suggested as a potential tool for combating harmful vegetable diseases with respect to promoting plant health and growth. B. subtilis has the ability to produce a diverse range of compounds to promote plant growth and suppress pathogen ingression, which makes it a potential candidate. Furthermore, B. subtilis enhances plant immunity against pathogen infection by triggering the response via induced systemic resistance (ISR). Additionally, B. subtilis promotes plant growth via different mechanisms of action, such as nitrogen fixation, phytohormonal production, and phosphate solubilisation. In this chapter, a comprehensive study on the application of B. subtilis has been emphasized, with a focus on uses in the promotion of plant growth and controlling vegetable crop health issues. It would undoubtedly assist vegetable growers in reducing their reliance on agrochemicals while also providing profound perceptions and highlights on the environmentally friendly management of vegetable diseases. Farmers will be benefitted from cost-effective management once they have a better understanding of the management strategy. © The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2024.
Benefits of Nano-Enhanced Biochar
(Apple Academic Press, 2024) Khalil Mseddi; Vishnu D. Rajput; Tatiana Minkina; Pradeep Kumar; Faiçal Brini
Recently, biochar (BC) has received attention as a useful additive for soil, and its production has increased substantially on a worldwide scale. The synthesis of biochar occurs as a byproduct of the thermochemical process of biomasses that takes place in the absence of or with restricted access to oxygen. Applications using biochar as a component for combating pollution and climate change are quite recent. From 2014, research on the environmentally responsible production of nano-biochar (also known as nano-BC) for use in agriculture and soil has been carried out as a result of the development of nanotechnology. Micro-sized BC with diameters of less than a micrometer (μm) and up to a nanometer (nm), also known as “dissolved” and “nano-BC,” is created during the carbonization process. A recent study produced nano-BC with a diameter of less than 5 nm. In this review paper, we reported the benefits of Nano-enhanced Biochar in all use domains following the enhanced physical-chemical properties compared to bulk biochar. © 2025 by Apple Academic Press, Inc.
Bioengineering Bacillus spp. for Sustainable Crop Production: Recent Advances and Resources for Biotechnological Applications
(Springer, 2024) Aurelio Ortiz; Estibaliz Sansinenea; Chetan Keswani; Tatiana Minkina; Satyendra Pratap Singh; Bhagwan Rekadwad; Rainer Borriss; Kathleen Hefferon; Trinh Xuan Hoat; Debasis Mitra; Pradeep Kumar Das Mohapatra; Periyasamy Panneerselvam
The goal of sustainable agriculture is to meet the rising need for food, while minimizing adverse impacts on the environment, protecting natural resources, and ensuring agricultural output over the long term. The pressing need to increase agricultural yield through sustainable agriculture is being emphasized. Several Bacillus species have been used as commercial biopesticides since they can act against plant pathogens by potentially suppressing them. At the same time, they can act as plant growth-promoting rhizobacteria and are known for their diverse characteristics and beneficial properties, making them potential candidates for use sustainable crop production programs. Knowledge of genetic information opens the door of possibility for understanding the way these microorganisms behave. By applying biotechnological tools to Bacillus, strategies can be adopted for the purpose of increasing the yield of crops and managing pests and pathogens that infect them. In this review, we identify the genes in the most significant Bacillus spp. that contribute to plant improvement. The most important biotechnological tools and advance computational approaches are described to provide an extended vision on this topic. However, increasing the crop production through application of beneficial microbial strains requires a multifaceted approach that considers ecological, economic, and social aspects. By implementing these strategies and practices, we can work towards a sustainable and resilient agricultural system that meets the growing food demand, while preserving the environment for future generations. © The Author(s) 2024.
Coping with the challenges of abiotic stress in plants: New dimensions in the field application of nanoparticles
(MDPI AG, 2021) Vishnu D. Rajput; Tatiana Minkina; Arpna Kumari; Harish; Vipin Kumar Singh; Krishan K. Verma; Saglara Mandzhieva; Svetlana Sushkova; Sudhakar Srivastava; Chetan Keswani
Abiotic stress in plants is a crucial issue worldwide, especially heavy-metal contami-nants, salinity, and drought. These stresses may raise a lot of issues such as the generation of reactive oxygen species, membrane damage, loss of photosynthetic efficiency, etc. that could alter crop growth and developments by affecting biochemical, physiological, and molecular processes, causing a significant loss in productivity. To overcome the impact of these abiotic stressors, many strategies could be considered to support plant growth including the use of nanoparticles (NPs). However, the majority of studies have focused on understanding the toxicity of NPs on aquatic flora and fauna, and relatively less attention has been paid to the topic of the beneficial role of NPs in plants stress response, growth, and development. More scientific attention is required to understand the behavior of NPs on crops under these stress conditions. Therefore, the present work aims to comprehensively review the beneficial roles of NPs in plants under different abiotic stresses, especially heavy metals, salinity, and drought. This review provides deep insights about mechanisms of abiotic stress alleviation in plants under NP application. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Correlation of the Effect of Native Bioagents on Soil Properties and Their Influence on Stem Rot Disease of Rice
(Multidisciplinary Digital Publishing Institute (MDPI), 2023) Sowmya Vanama; Maruthi Pesari; Gobinath Rajendran; Uma Devi Gali; Santosha Rathod; Rajanikanth Panuganti; Srivalli Chilukuri; Kannan Chinnaswami; Sumit Kumar; Tatiana Minkina; Estibaliz Sansinenea; Chetan Keswani
Soil is a crucial component for plant growth, as it provides water, nutrients, and mechanical support. Various factors, such as crop cultivation, microflora, nutrient addition, and water availability, significantly affect soil properties. Maintaining soil health is important, and one approach is the introduction of native organisms with multifaceted activities. The study evaluates the effects of introducing these microbes (Trichoderma asperellum strain TAIK1, Bacillus cabrialesii strain BIK3, Pseudomonas putida strain PIK1, and Pseudomonas otitidis strain POPS1) and their consortium, a combination of four bioagents, on soil health, plant growth, and the incidence of stem rot disease caused by Sclerotium oryzae in rice. Upon treatment of soil with the consortium of the four native bioagents mentioned above through seed treatment or soil application, variations/increases in the chemical properties of the soil were observed, viz., pH (8.08 to 8.28), electrical conductivity (EC) (0.72 to 0.75 d S m−1), organic carbon (OC) (0.57 to 0.68 %), available soil nitrogen (SN) (155 to 315 kg/ha), soil phosphorus (SP) (7.87 to 24.91 kg/ha), soil potassium (SK) (121.29 to 249.42 kg/ha), and soil enzymes (urease (0.73 to 7.33 µg urea hydrolyzed g−1 soil h−1), acid and alkaline phosphatase (0.09 to 1.39 and 0.90 to 1.78 µg of p-nitrophenol released g−1 soil h−1), and dehydrogenase (0.14 to 16.44 mg triphenyl formazan (TPF) produced g−1 soil h−1)), compared to untreated soil. Treatment of seeds with the consortium of four native bioagents resulted in a significant increase in plant height (39.16%), the number of panicles (30.29%), and average grain yield (41.36%) over control plants. Under controlled conditions, the bioagent-treated plants showed a 69.37% reduction in stem rot disease. The findings of this study indicate a positive correlation between soil properties (pH, EC, OC, SN, SP, SK, and soil enzymes) and plant growth (shoot and root length, fresh and dry weight) as well as a highly negative association of soil properties with stem rot disease severity. The results suggest that using native bioagents as a management strategy can control stem rot disease and enhance crop productivity, while reducing reliance on chemical management. These findings provide valuable insights into the development of sustainable agricultural practices that maximize productivity by minimizing negative environmental impacts, which promotes soil health, plant growth, and disease management. © 2023 by the authors.
Defense Inducers Mediated Mitigation of Bacterial Canker in Tomato through Alteration in Oxidative Stress Markers
(MDPI, 2022) Ruchi Tripathi; Karuna Vishunavat; Rashmi Tewari; Sumit Kumar; Tatiana Minkina; Ugo De Corato; Chetan Keswani
The bacterial canker disease of tomato caused by Clavibacter michiganensis subsp. michiganensis (Cmm) has been reported to adversely affect the tomato cultivation in the NE hilly regions of India. Defense inducers such as salicylic acid (SA), isonicotinic acid (INA), benzothiadiazole (BTH) and lysozyme were used as prophylactic and curative sprays at different concentrations to test their efficacy in inducing resistance in tomato plants against Cmm under protected conditions. The induced resistance was studied through the alteration in the activities of oxidative stress marker enzymes (PAL, PO, PPO, TPC and PR-2 protein), hydrogen peroxide formation in leaf tissues and lignin accumulation in stem tissues, as well as through the reduction in disease severity under glasshouse conditions. The results of the present study revealed that the enzymatic activity, hydrogen peroxide formation and lignin production were significantly higher in the BTH (500 ppm)-treated leaves than in those observed in the control. The lowest disease incidence was recorded when BTH was applied as a prophylactic spray (27.88%) in comparison to being applied as a curative spray (53.62%), thereby suggesting that a defense inducer, BTH, shows antibacterial activity against Cmm, reduces disease incidence severity and induces defense responses in the tomato plant. © 2022 by the authors.
Effect of zno nanoparticles on growth and biochemical responses of wheat and maize
(MDPI, 2021) Akansha Srivastav; Deepak Ganjewala; Rakesh Kumar Singhal; Vishnu D. Rajput; Tatiana Minkina; Marina Voloshina; Sudhakar Srivastava; Manoj Shrivastava
Zinc is an essential element that is also renowned for widespread contamination and toxicity at high concentrations. The present study was carried out to analyze the responses induced by lower, as well as higher, doses of zinc (0–200 mg/L), in the form of zinc oxide nanoparticles (ZnO NPs) in wheat and maize, for a period of 21 days. Accumulation of zinc increases with increasing Zn doses in both wheat and maize, with higher doses being in wheat (121 mg/kg in root and 66 mg/kg in shoot) than in maize (95 mg/kg in root and 48 mg/kg in shoot). The activity of alpha-amylase showed increase, while that of dehydrogenase decline, in response to ZnO NPs. The length and biomass of plants and photosynthetic pigments increased slightly upon ZnO NPs supply. Malondialdehyde content showed a progressive increase in root and shoot of both plants. However, in response, antioxidant enzymes (superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase, and catalase) showed increase up to lower concentrations (100 mg/L) of ZnO NPs but decline variably at higher levels (150–200 mg/L) in wheat and maize. The results suggest that lower supply of ZnO NPs (100 mg/L) could be stimulatory to the growth of plants and can be recommended as a Zn fertilizer source for crop production. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Efficient Catalytic Degradation of Selected Toxic Dyes by Green Biosynthesized Silver Nanoparticles Using Aqueous Leaf Extract of Cestrum nocturnum L.
(MDPI, 2022) Pradeep Kumar; Jyoti Dixit; Amit Kumar Singh; Vishnu D. Rajput; Pooja Verma; Kavindra Nath Tiwari; Sunil Kumar Mishra; Tatiana Minkina; Saglara Mandzhieva
In the present study, the catalytic degradation of selected toxic dyes (methylene blue, 4-nitrophenol, 4-nitroaniline, and congo red) using biosynthesized green silver nanoparticles (AgNPs) of Cestrum nocturnum L. was successfully performed. These AgNPs are efficiently synthesized when a reaction mixture containing 5 mL of aqueous extract (3%) and 100 mL of silver nitrate (1 mM) is exposed under sunlight for 5 min. The synthesis of AgNPs was confirmed based on the change in the color of the reaction mixture from pale yellow to dark brown, with maximum absorbance at 455 nm. Obtained NPs were characterized by different techniques, i.e., FTIR, XRD, HR-TEM, HR-SEM, SAED, XRD, EDX, AFM, and DLS. Green synthesized AgNPs were nearly mono-dispersed, smooth, spherical, and crystalline in nature. The average size of the maximum number of AgNPs was 77.28 ± 2.801 nm. The reduction of dyes using a good reducing agent (NaBH4) was tested. A fast catalytic degradation of dyes took place within a short period of time when AgNPs were added in the reaction mixture in the presence of NaBH4. As a final recommendation, Cestrum nocturnum aqueous leaf extract-mediated AgNPs could be effectively implemented for environmental rehabilitation because of their exceptional performance. This can be utilized in the treatment of industrial wastewater through the breakdown of hazardous dyes. © 2022 by the authors.
Fostering plant growth performance under drought stress using rhizospheric microbes, their gene editing, and biochar
(Springer, 2024) Prabhat K. Chauhan; Sudhir K. Upadhyay; Vishnu D. Rajput; Padmanabh Dwivedi; Tatiana Minkina; Ming Hung Wong
Stress due to drought lowers crop yield and frequently leads to a rise in food scarcity. Plants' intricate metabolic systems enable them to tolerate drought stress, but they are unable to handle it well. Adding some external, environmentally friendly supplements can boost plant growth and productivity when it comes to drought-stressed plants. In order to prevent the detrimental effects of drought in agricultural regions, environmentally friendly practices must be upheld. Plant growth-promoting rhizobacteria (PGPR) can exhibit beneficial phytostimulation, mineralization, and biocontrol activities under drought stress. The significant impact of the PGPR previously reported has not been accepted as an effective treatment to lessen drought stress. Recent studies have successfully shown that manipulating microbes can be a better option to reduce the severity of drought in plants. In this review, we demonstrate how modifying agents such as biochar, PGPR consortia, PGPR, and mycorrhizal fungi can help overcome drought stress responses in crop plants. This article also discusses CRISPR/Cas9-modifiable genes, increase plant’s effectiveness in drought conditions, and increase plant resistance to drought stress. With an eco-friendly approach in mind, there is a need for practical management techniques having potential prospects based on an integrated strategy mediated by CRISPR-Cas9 editing, PGPR, which may alleviate the effects of drought stress in crops and aid in achieving the United Nation Sustainable Development Goals (UN-SDGs-2030). © 2023, The Author(s), under exclusive licence to Springer Nature B.V.
Global footprints of organochlorine pesticides: a pan-global survey
(Springer Science and Business Media B.V., 2022) Chetan Keswani; Hagera Dilnashin; Hareram Birla; Partha Roy; Rakesh K. Tyagi; Dheer Singh; Vishnu D. Rajput; Tatiana Minkina; Surya P. Singh
Organochlorine pesticides (OCPs) are ubiquitous environmental contaminants widely used all over the world. These chlorinated hydrocarbons are toxic and often cause detrimental health effects because of their long shelf life and bioaccumulation in the adipose tissues of primates. OCP exposure to humans occurs through skin, inhalation and contaminated foods including milk and dairy products, whereas developing fetus and neonates are exposed through placental transfer and lactation, respectively. In 1960s, OCPs were banned in most developed countries, but because they are cheap and easily available, they are still widely used in most third world countries. The overuse or misuse of OCPs has been rising continuously which pose threats to environmental and human health. This review reports the comparative occurrence of OCPs in human and bovine milk samples around the globe and portrays the negative impacts encountered through the long history of OCP use. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.
Green Nanofertilizers – The Need for Modern Agriculture, Intelligent, and Environmentally-Friendly Approaches
(Polskie Towarzystwo Inzynierii Ekologicznej (PTIE), 2024) Abdel Rahman Mohammad Al Tawaha; Abhishek Singh; Vishnu D. Rajput; Ashi Varshney; Shreni Agrawal; Karen Ghazaryan; Tatiana Minkina; Omar Mahmoud Al Zoubi; Talaat Habeeb; Lysenko Dionis; Hanan Aref Hasan; Samar Shawaqfeh
The distinctive qualities and wide array of possible applications of nanotechnology have garnered considerable attention. Nanotechnology offers a groundbreaking way for expanding agricultural output that is also ecologically benign, helpful to living things, and economically priced—all without losing quality. There is a growing trend towards using eco-friendly technologies as substitutes for conventional agricultural inputs, such as fertilizers and insecticides. With the aid of nanotechnology, the confines of conventional farming techniques can be overcome. As a result, it becomes essential for investigators to devote their energies to the noteworthy nanoparticles (NPs) in agriculture investigations that have been distributed. It offered a fresh perspective on the development and application of nanoparticles as nanofertilizers and nano-pesticides in agriculture and a way to heighten bio-factor execution. Furthermore, we discuss the relations of NPs with plants, the perils and putrefaction of nanomaterials in plants, and the utility of NPs in the reduction of stress triggered by heavy metal toxicity and abiotic factors. It is imperative that nano-fertilizers are practiced to reduce the environmental maltreatment caused by conventional, inorganic fertilizers. Nano-fertilizers are more sensitive and have the ability to penetrate the epidermis, empowering them to promote nutrient consumption efficiency while reducing nutrient overabundance. A study found that NPs may cause oxidative stress symptoms in higher plants if they adhere to cell surfaces or organelles. Understanding the benefits and drawbacks of using nano-fertilizers instead of conventional fertilizers is valuable, and it is the purpose of this book chapter to provide this information. © 2024, Polskie Towarzystwo Inzynierii Ekologicznej (PTIE). All rights reserved.
Green Synthesis of Nanofertilizers and Their Application for Crop Production
(Springer Science and Business Media B.V., 2024) Abhishek Singh; Ragini Sharma; Vishnu D. Rajput; Karen Ghazaryan; Tatiana Minkina; Abdel Rahman Mohammad Al Tawaha; Ashi Varshney
The unique characteristics and broad range of nanotechnology’s potential uses have garnered considerable attention. Nanotechnology is a novel method for boosting agricultural output without compromising on quality while also being safe for the environment, useful to living organisms, and affordable. Eco-friendly technologies are gaining prominence as viable alternatives to conventional agricultural inputs like fertilizers and insecticides. The limitations of traditional farming methods can be addressed with the use of nanotechnology. Hence, the most recent research on using nanoparticles (NPs) in farming should be prioritized. This study offered a fresh viewpoint on the biological development of NPs, their uses in agriculture as nanofertilizers and nanopesticides, and how they can improve the performance of biofactors. We also provide recent research on the interactions of NPs with plants, the hazards and fate of nanomaterials in plants, and the role of NPs in the mitigation of stress brought on by abiotic factors and heavy metal toxicity. To reduce the environmental harm caused by conventional, inorganic fertilizers, nanofertilizer application is crucial. Because of their heightened responsiveness and capacity to pierce the epidermis, nanofertilizers can reduce nutrient surplus while increasing nutrient usage efficiency. The importance of NPs in mitigating abiotic stress and heavy metal toxicity was also established. Some research has shown that NPs can be hazardous to higher plants by depositing themselves on the cell surface or in organelles, hence generating signs of oxidative stress. It is crucial to comprehend the advantages and disadvantages of using nanofertilizers as an alternative to conventional fertilizers, as we have done in this book chapter. © 2024, The Author(s), under exclusive license to Springer Nature Switzerland AG.
Hydrogel-based Trichoderma formulation effects on different varieties of rice under rainfed condition of Indo-Gangetic Plains
(Springer Science and Business Media B.V., 2022) K. Dujeshwer; Ram Kumar Singh; Hanuman Singh Jatav; Rajendra Lakpale; Mujahid Khan; Vishnu D. Rajput; Tatiana Minkina
Increased global CO2 emissions may result in erratic weather conditions, especially uncertain, pertaining to rainfall uncertainties and temperature anomalies, and could reduce India’s overall rice production by 3–10% under medium- to high-emission scenarios. The water crises nowadays have been prioritized as one of the top five global risks. Further, the uncertainties in rice production due to climate change will be more than just rice yield reductions. Several adoption strategies such as direct seeding, selecting water stress-tolerant varieties, enhancing soil water-holding capacity and improving crop management practices, are suggested to address the risks of rice production. Keeping in view the above fact, a field experiment was initiated during kharif season of 2015 and 2016 at Agricultural Research Farm (BHU), Varanasi, Uttar Pradesh (India), to assess the effect of super-absorbent polymer (hydrogel) and Trichoderma in rice varieties with six hydrogel-based Trichoderma-formulated treatments. The results indicated that Trichoderma was found effective in improving crop growth, yield, nutrient uptake and water use efficiency with the application of hydrogel. It was also found that soil amendment with hydrogel at 2 g m−2 and sowing of Trichoderma-treated seed at 10 g kg−1 seed significantly improved the crop growth parameters (viz. shoot dry weight by 6.45%), yield parameters (viz. number of productive tillers by 12.32%, number of grains per panicle by 8.26%), nutrients uptake and water use efficiency (by 24.15%) over control. The present study reveals that the use of hydrogel with Trichoderma fungus is found effective in enhancing the growth and yield parameters of rice in Indo-Gangetic Plains (IGPs). © 2021, The Author(s), under exclusive licence to Springer Nature B.V.
Identification and validation of core genes as promising diagnostic signature in hepatocellular carcinoma based on integrated bioinformatics approach
(Nature Research, 2022) Pradeep Kumar; Amit Kumar Singh; Kavindra Nath Tiwari; Sunil Kumar Mishra; Vishnu D. Rajput; Tatiana Minkina; Simona Cavalu; Ovidiu Pop
The primary objective of this investigation was to determine the hub genes of hepatocellular carcinoma (HCC) through an in silico approach. In the current context of the increased incidence of liver cancers, this approach could be a useful prognostic biomarker and HCC prevention target. This study aimed to examine hub genes for immune cell infiltration and their good prognostic characteristics for HCC research. Human genes selected from databases (Gene Cards and DisGeNET) were used to identify the HCC markers. Further, classification of the hub genes from communicating genes was performed using data derived from the targets' protein–protein interaction (PPI) platform. The expression as well as survival studies of all these selected genes were validated by utilizing databases such as GEPIA2, HPA, and immune cell infiltration. Based on the studies, five hub genes (TP53, ESR1, AKT1, CASP3, and JUN) were identified, which have been linked to HCC. They may be an important prognostic biomarker and preventative target of HCC. In silico analysis revealed that out of five hub genes, the TP53 and ESR1 hub genes potentially act as key targets for HCC prevention and treatment. © 2022, The Author(s).