Browsing by Author "Tejaswini Dhanaji Patil"

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Correction to: Production, optimization, scale up and characterization of polyhydoxyalkanoates copolymers utilizing dairy processing waste (Scientific Reports, (2024), 14, 1, (1620), 10.1038/s41598-024-52098-0)
(Nature Research, 2024) Tejaswini Dhanaji Patil; Saptaneel Ghosh; Aparna Agarwal; Sanjay Kumar Singh Patel; Abhishek Dutt Tripathi; Dipendra Kumar Mahato; Pradeep Kumar; Petr Slama; Ales Pavlik; Shafiul Haque
Correction to: Scientific Reportshttps://doi.org/10.1038/s41598-024-52098-0, published online 18 January 2024 The Author Contributions section in this Article was incomplete. “T.D.P., A.D.T., P.S., A.P., S.H. and S.K.S.P. conceptualized the experiment. T.D.P., A.D.T. and A.A did the methodology and validation. T.D.P., A.D.T. conducted the formal analysis and investigation. T.D.P., A.D.T., and S.K.S.P. wrote and prepared the original draft. A.D.T., P.S., A.P., S.H., and S.K.S.P. conducted the writing, review and editing. A.A. helped in visualization. A.D.T. and A.A. supervised the experiment. S.H. and D.K.M. edited the manuscript. T.D.P., A.D.T. and A.A. performed analytical work. All authors have read and agreed to the published version of the manuscript.” now reads, “T.D.P., A.D.T., P.S., A.P., S.H. and S.K.S.P. conceptualized the experiment. T.D.P., A.D.T. and A.A. did the methodology and validation. T.D.P., A.D.T. conducted the formal analysis and investigation. T.D.P., A.D.T., and S.K.S.P. wrote and prepared the original draft. A.D.T., P.S., A.P., S.H., and S.K.S.P. conducted the writing, review, and editing. A.A. helped in visualization. A.D.T. and A.A. supervised the experiment. S.H. and D.K.M. edited the manuscript. T.D.P., A.D.T., P.K., and A.A. performed analytical work. All authors have read and agreed to the published version of the manuscript.” The original Article has been corrected. © The Author(s) 2024.
Production, optimization, scale up and characterization of polyhydoxyalkanoates copolymers utilizing dairy processing waste
(Nature Research, 2024) Tejaswini Dhanaji Patil; Saptaneel Ghosh; Aparna Agarwal; Sanjay Kumar Singh Patel; Abhishek Dutt Tripathi; Dipendra Kumar Mahato; Pradeep Kumar; Petr Slama; Ales Pavlik; Shafiul Haque
The microbial biotransformation using low-cost feedstock to produce biopolymers (degradable), an alternative to petrochemical-based synthesis plastics (non-degradable), can be a beneficial approach towards sustainable development. In this study, the dairy industry processes waste (whey) is used in polyhydroxyalkanoate (PHA) copolymer production. Initial screening suggested that Ralstonia eutropha produced higher PHA as compared to Bacillus megaterium. A central composite rotatable design-based optimization using two process variables (amino acid and tween-80) concentration remarkably influenced PHA co-polymer production under physiological conditions of pH (7), temperature (37 °C), and agitation rate of 150 rpm. High polyhydroxybutyrate (PHB) mass fraction yield of 69.3% was observed as compared to predicted yield of 62.8% from deproteinized whey as feed. The combination of tryptophan (50 mg L−1) and tween-80 (3 mL−1) enhanced R. eutropha mass gain to 6.80 g L−1 with PHB contents of 4.71 g L−1. Further, characterization of PHA and its copolymers was done by ESI–MS, FTIR, and TEM. On upscaling up to 3.0 L, the PHA contents and yields were noted as quite similar by R. eutropha. This study demonstrates that dairy waste processing waste can be potentially utilized as inexpensive feed for producing high content of biopolymers to develop a sustainable system of waste management. © 2024, The Author(s).
Tailoring PHA copolymer types via amino acid supplementation in dairy byproduct media
(Nature Research, 2025) Tejaswini Dhanaji Patil; Preetam Bannerjee; Aparna Agarwal; Abhishek Dutt Tripathi; Veena Paul; Ankita Hooda; Mansour K. Ghatasheh; Dipendra Kumar Mahato
The microbial biotransformation of low-cost feedstock to generate biodegradable biopolymers, as an alternative to synthetic plastics, is a promising strategy for sustainable development. The study investigated the biotransformation of dairy byproduct (whey) into polyhydroxyalkanoate (PHA) copolymers with the supplementation of amino acids using Ralstonia eutropha. The sulphur-containing (cystine, cysteine, methionine) and aromatic (phenylalanine, tryptophan, and tyrosine) amino acids were supplemented. The highest yield of 37.1195% was obtained with tryptophan supplementation. The characterisation of PHA and its copolymers was conducted using ESI–MS and FTIR. This study demonstrates that dairy byproducts can potentially serve as a cost-effective feedstock for producing high concentrations of biopolymers with amino acid supplementation, thereby promoting a sustainable waste management system. © The Author(s) 2025.