Browsing by Author "Thakur Ji"

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Observations On Nuclear Division in vegetative hyphae of aquatic fungi
(Kluwer Academic Publishers, 1973) Ram Dayal; Thakur Ji
Achlya aquatica, A. prolifera and A. racemosa are additional examples of fungi in whose vegetative mycelium, the nuclei do not divide in a manner directly comparable to ordinary mitosis. During division the entire nucleus, which consists of variously shaped chromatin and the nucleolus, becomes angular and elongates. The chromatin separates into two portions, which are situated at opposite ends of the elongating nucleus. As division proceeds the nuclei separate. Each sister nucleus sonsists of chromatin and a portion of the divided nucleolus. Spindless, chromosomal filaments or metaphase plates could not be observed during division. © 1973 Dr. W. Junk b.v. Publishers.
Studies in aquatic fungi of Varanasi - II. A new species of Achlya with notes on other species
(Kluwer Academic Publishers, 1969) Ram Dayal; Thakur Ji
A morphological and taxonomic investigation of the aquatic Phycomycetous flora of the Varanasi district was made during the parts of the summers of 1965-66. Among the many aquatic fungal taxa identified and studied, only two viz. Achlya rodrigueziana and A. crenulata were new records from this country. A modified and well illustrated description of A. dubia from Chaudhuri &Kochhar (1935) has been given. A new species of Achlya is also described. © 1969 Dr. W. Junk N.V.
Studies in the life cycle of Allomyces javanicus Kniep
(Kluwer Academic Publishers, 1971) Thakur Ji; R. Dayal
The life cycle of Allomyces javanicus was studied with the hanging drop method under laboratory conditions. The isolate has a life cyrle similar to that already described in A. javanicus and A. arbuscula. Planonts from resistant sporangia do not germinate directly to produce gametophytic plants, but on the contrary, the latter germinate to produce plants like their immediate parents. Female and male gametes growing in the gametangia of relatively young sexual mycelia develop into asexual mycelia after conjugation, but some of the female gametes after germination develop into asexual mycelia without conjugation. © 1971 Dr. W. Junk Publishers.
Studies in the Mitochondria of Allomyces Butler
(1970) Thakur Ji
Mitochondria of the two species of Allomyces, viz., A. javanicus, and A. moniliformis were investigated. Various forms of mitochondria were present in varying proportions. In isotonic solutions of NaCl, the mitochondria disintegrated into granules, but in hypertonic saline solution cells were plasmolysed at first but recovered later on. Mitochondria in such cells changed their shape, fragmented, became vesiculated and disappeared. In 0.5M sucrose solution, they quickly fragmented into small spheres, averaging 1 micron in diameter. Temperature of 10°±1° to 45°C had seemingly no effect on mitochondria. At higher temperatures, viz., 50°C, the mitochondrial movement ceased and at 75°C they broke up into beads and disappeared. Mitochondrial orientation was determined by the direction of cytoplasmic streaming. Small granules as normal mitochondria were stained red after 48 to 72 hours with tetrazolium chloride of 0.005 per cent concentration. Higher concentrations were lethal. Mitochondria were stained supravitally with Janus green B Hoechst as sky blue colour and reduction of the dye was observed after sometime. Amongst all the fixatives tested, Regaud's formol-bichromate was found to be the most successful. © 1970, Japan Mendel Society, International Society of Cytology. All rights reserved.
The occurrence and distribution of aquatic fungi in certain ponds of Varanasi
(Kluwer Academic Publishers, 1966) Ram Dayal; Thakur Ji
A survey of the Phycomycetous flora of the three types of the collecting stations in Varanasi was made for about ten months. The following observations were made: 1. An attempt was made to correlate the distribution of fungi in a group of small ponds, lakes with the idea of their occurrence, periodicity and environmental factors involved in the growth of aquatic fungi. 2. Sixteen species were recorded belonging to the Saprolegniales, Blastocladiales and Peronosporales. 3. There is distinct seasonal periodicity of growth which is mainly restricted to winter months. 4. The results indicate that the rarity of the many aquatic Phycomycetes may not be only due to specificity of substratum but also to temperature and the time needed for the maturation of the oospores. 5. The hydrogen-ion concentration of the ponds surveyed, has been measured, which supports the view of wide occurrence of members of Saprolegniales over a wide range of pH with the exception of Allomyces sp. and Pythium sp. which probably prefer the lower alkalinity of water. © 1966 Dr. W. Junk Publishers.