Browsing by Author "Trinh Xuan Hoat"

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A new phytoplasma strain associated with the sugarcane white leaf disease in Vietnam
(Technology Society of Basic and Applied Sciences, 2020) Mai Van Quan; Nguyen Van Liem; Le Quang Man; Ngo Quang Huy; Le Xuan Vi; Ha Viet Cuong; Duong Thi Nguyen; Hoang Thi Bich Thao; Nguyen Viet Hung; Nguyen Duc Huy; Phyllis G. Weintraub; Chetan Keswani; Le Thi Hang; Nguyen Manh Hung; Trinh Xuan Hoat
Sugarcane white leaf (SCWL) disease is one of the most important diseases of sugarcane in Vietnam. The presence of phytoplasmas in phloem tubes of infected plants was confirmed by DAPI staining. A nested PCR assay using R16mF2/R16mR1 and R16F2n/R16R2 primer pairs amplified products with approximate length of 1,250 bp from the symptomatic sugarcane samples. The sequence analysis indicated that the phytoplasma strains from Khanh Hoa province shared 100% sequence identity with previously sequenced SCWL phytoplasma strains that were related to ‘Candidatus Phytoplasma oryzae’ (16SrXI). The virtual RFLP patterns of a 1,127 bp sequence obtained with the restriction enzymes BfaI, BstUI, EcoRI, HaeIII, HinfI, HpaI, RsaI and TaqI indicate that this SCWL-associated phytoplasma from Vietnam is a different strain from those in the 16SrXI described ribosomal subgroups. The two insect species, Matsumuratettix hiroglyphicus and Yamatotettix flavovittatus, identified as the vectors of SCWL in Thailand, were identified and found to be infected also in the SCWL symptomatic fields in Vietnam. The transmission experiments from SCWL-infected leafhoppers to healthy sugarcane and to insect feeding medium failed, suggesting that the infected planting material is the major transmission manner of SCWL disease in Vietnam. © 2020, Technology Society of Basic and Applied Sciences. All rights reserved.
Applications of agriculturally important microorganisms for sustainable crop production
(Elsevier, 2020) Hagera Dilnashin; Hareram Birla; Trinh Xuan Hoat; H.B. Singh; S.P. Singh; Chetan Keswani
Conventional agriculture cannot fulfill all the requirements expected from agriculture. Whereas, sustainable agriculture can help us achieve all the agricultural needs. Sustainable agriculture fulfills the agricultural needs by using the resources from the environment to the maximum, without damaging the natural resources. Hence, this technique provides safe and healthy products from agriculture without causing damage to the environment. The microbial population plays a key role in determining the strength and productivity of agro-ecosystems as they directly affect the fundamental processes related to them. Ongoing research areas help in understanding the diversity, dynamics and importance of soil microbial communities and how they can help in increasing the agricultural productivity. Although, here is the only effects of plant growth promoting rhizobacteria (PGPR) and cyanobacteria on sustainable agriculture is addressed. An agricultural system which is sustainable, improves human health, causes no damage to the natural environment and meets the global food requirement can be referred to as an ideal system. Use of bio-fertilizers can help reduce environmental pollution, urea consumption and soil depletion. The PGPR and cyanobacteria model systems can deliver genetic constituents and bioactive compounds which affect sustainable agriculture and hence environment. PGPR and cyanobacteria can improve crop production and soil health, utilizing sustainable technique. © 2020 Elsevier Inc. All rights reserved.
Bioengineering Bacillus spp. for Sustainable Crop Production: Recent Advances and Resources for Biotechnological Applications
(Springer, 2025) Aurelio Ortiz; Estibaliz Sansinenea; Keswani Chetan; Tatiana Mikhailovna Minkina; Satyendra Pratap Singh; Bhagwan Narayan Rekadwad; Rainer Borriss; Kathleen Laura Hefferon; Trinh Xuan Hoat; Debasis Mitra; Pradeep Kumar Das Mohapatra; Periyasamy Panneerselvam
The goal of sustainable agriculture is to meet the rising need for food, while minimizing adverse impacts on the environment, protecting natural resources, and ensuring agricultural output over the long term. The pressing need to increase agricultural yield through sustainable agriculture is being emphasized. Several Bacillus species have been used as commercial biopesticides since they can act against plant pathogens by potentially suppressing them. At the same time, they can act as plant growth-promoting rhizobacteria and are known for their diverse characteristics and beneficial properties, making them potential candidates for use sustainable crop production programs. Knowledge of genetic information opens the door of possibility for understanding the way these microorganisms behave. By applying biotechnological tools to Bacillus, strategies can be adopted for the purpose of increasing the yield of crops and managing pests and pathogens that infect them. In this review, we identify the genes in the most significant Bacillus spp. that contribute to plant improvement. The most important biotechnological tools and advance computational approaches are described to provide an extended vision on this topic. However, increasing the crop production through application of beneficial microbial strains requires a multifaceted approach that considers ecological, economic, and social aspects. By implementing these strategies and practices, we can work towards a sustainable and resilient agricultural system that meets the growing food demand, while preserving the environment for future generations. © The Author(s) 2024.
Bioengineering Bacillus spp. for Sustainable Crop Production: Recent Advances and Resources for Biotechnological Applications
(Springer, 2024) Aurelio Ortiz; Estibaliz Sansinenea; Chetan Keswani; Tatiana Minkina; Satyendra Pratap Singh; Bhagwan Rekadwad; Rainer Borriss; Kathleen Hefferon; Trinh Xuan Hoat; Debasis Mitra; Pradeep Kumar Das Mohapatra; Periyasamy Panneerselvam
The goal of sustainable agriculture is to meet the rising need for food, while minimizing adverse impacts on the environment, protecting natural resources, and ensuring agricultural output over the long term. The pressing need to increase agricultural yield through sustainable agriculture is being emphasized. Several Bacillus species have been used as commercial biopesticides since they can act against plant pathogens by potentially suppressing them. At the same time, they can act as plant growth-promoting rhizobacteria and are known for their diverse characteristics and beneficial properties, making them potential candidates for use sustainable crop production programs. Knowledge of genetic information opens the door of possibility for understanding the way these microorganisms behave. By applying biotechnological tools to Bacillus, strategies can be adopted for the purpose of increasing the yield of crops and managing pests and pathogens that infect them. In this review, we identify the genes in the most significant Bacillus spp. that contribute to plant improvement. The most important biotechnological tools and advance computational approaches are described to provide an extended vision on this topic. However, increasing the crop production through application of beneficial microbial strains requires a multifaceted approach that considers ecological, economic, and social aspects. By implementing these strategies and practices, we can work towards a sustainable and resilient agricultural system that meets the growing food demand, while preserving the environment for future generations. © The Author(s) 2024.
Biological control of fusarium root rot of Indian mulberry (Morinda officinalis How.) with consortia of agriculturally important microorganisms in Viet Nam
(Springer International Publishing, 2019) Duong Thi Nguyen; Nguyen Chi Hieu; Nguyen Viet Hung; Hoang Thi Bich Thao; Chetan Keswani; Pham Van Toan; Trinh Xuan Hoat
Background: Fusarium root rot disease in Indian mulberry (Morinda officinalis How.) (FRRBK), caused by Fusarium proliferatum (FP), is widespread and responsible for serious economic losses in Viet Nam. The efficacy of a new bio-product named MICROTECH-1(NL) is compared with other commercial products for suppression of FP under in vitro, pot, nursery as well as in the field conditions. Results: In in vitro antagonistic assay, MICROTECH-1(NL) significantly inhibited the mycelial growth of FP (72.38%). Under pot conditions, the efficacy of all the bio-products was significantly higher when applied prior to pathogen inoculation. The disease severity of treatments with double application of MICROTECH-1(NL) (applied both in the nursery and in the pot soil) was only 15.56%, significantly lower than control (80%). Thus, the application of MICROTECH-1(NL) significantly reduced the incidence of FP and markedly increased the number of plant beneficial bacteria and actinobacteria in rhizoplane of M. officinalis compared to untreated control. In the field conditions, double application of MICROTECH-1(NL) (both in the nursery and in the field soils) significantly decreased disease severity in comparison to single application in nursery or field. Conclusion: The most effective treatment was double application of MICROTECH-1(NL), which significantly reduced the disease severity and FP population in roots of M. officinalis and increased the population of plant beneficial microbes.[Figure not available: see fulltext.]. © 2019, The Author(s).
Trichoderma spp. mediated induction of systemic defense response in brinjal against Sclerotinia sclerotiorum
(Elsevier Ltd, 2021) Satyendra Pratap Singh; Chetan Keswani; Surya Pratap Singh; Estibaliz Sansinenea; Trinh Xuan Hoat
Induction of resistance to pathogen is associated with the colonization of root by Trichoderma spp. has been attributed as one of the major mechanisms contributing to pathogenic invasion. The present study sheds light on the defense network of brinjal plant bioprimed with Trichoderma spp. challenged with Sclerotinia sclerotiorum. Plants treated with dual inoculation of Trichoderma harzianum and Trichoderma asperellum triggered further synthesis of TPC under S. sclerotiorum challenge with maximum increment recorded at 72 hours. In consortium treated and pathogen challenged plants, a higher amount of shikimic acid was observed at 72 hours, whereas other phenolics showed little differences among the treatments. The consortium treatment showed significantly higher defense related enzymes (Phenylalanine Ammonia Lyase, Peroxidase and Polyphenol Oxidase) activity than other treatments. The study signifies how Trichoderma spp. reprograms the host's defense network to provide robust protection against S. sclerotiorum. In the present case, overall protection was provided to the brinjal plants against the attack of S. sclerotiorum. © 2021 The Author(s)