Browsing by Author "Tushar Ranjan"

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Isolation and characterization of a novel nitrogen fixer Beijerinckia fluminensis strain BAUMS11 from litchi (Litchi chinensis L.) rhizosphere
(Applied and Natural Science Foundation, 2024) Mahendra Singh; Santosh Kumar; Dhirendra Kumar Singh; Tushar Ranjan; Alok Kumar Pandey
Indiscriminate use of mineral fertilizers has a broad negative impact on soil health. Because of the above, there is an urgent need to search for natural organic alternatives, including using soil microbial resources to replenish soil nutrients for enhanced Agri productivity vis a vis sustainably maintaining soil health. The nitrogen-fixing rhizobacteria (NFR) are such type of bacteria which fix gaseous atmospheric nitrogen in the soil and in nodules of certain plant species in considerable amounts that are readily available for plants' uptake and may be considered as a viable alternative source of mineral nitrogen application. The present study was conducted to isolate the most potent nitrogen-fixing bacteria from the litchi rhizosphere. Hence, Five NFR (NFR1 to NFR5) were isolated from the rhizosphere of litchi orchard of Bihar Agricultural University, Sabour, Bhgalpur, India, based on their ability to fix atmospheric nitrogen in a nitrogen-free mineral salt medium. NFR2 was found to be the most potent in fixing atmospheric nitrogen (11.31 mg N per gram carbon source)among all the isolated rhizobacterial strains. Hence, on the basis of biological nitrogen fixation ability, the isolate NFR2 was subjected to 16S ribosomal RNA (16S rRNA) gene sequencing for molecular characterization. Based on 16S rDNA sequence analysis, NFR2 showed the closest sequence homology with Beijerinckia fluminensis and was identified and reported as Beijerinckia fluminensis strain BAUMS11, Accession number MN533953. The study noticeably indicated that the B. fluminensis strain BAUMS11 was found most efficient in fixing gaseous atmospheric nitrogen and may be used for the manufacturing of nitrogenous biofertilizer, which can fix atmospheric nitrogen to the tune-up to 30 kg N ha-1yr-1. © Author (s).
Recent advances in the development of disease-resistant plants utilizing genetic engineering approaches
(Apple Academic Press, 2024) Bishun Deo Prasad; Sangita Sahni; Diksha Kumari; Anand Kumar; Tushar Ranjan; Sunayana Rathi; Surojit Sen
Transgenic technologies are the only way to bridge the gap between an ever-increasing human population and their hunger, as well as diminishing crop productivity due to changing climatic conditions. Genetic engineering contributes directly to agricultural productivity by providing superior varieties with enhanced resistance to biotic stresses. Pathogen-derived resistance (PDR) refers to the resistance obtained from a pathogenic virus using viral protein like coat protein (CP), replicase protein (RP), and movement protein (MP). The biomolecules AMPs, PR proteins, RIPs, and enzymes involved in secondary metabolite biosynthesis (phytoalexins and phytoanticipins) also play a role in plant defense. Further, RNA interference (RNAi) is considered as a means of regulating immunity in GM disease-resistant plants. The development of the RNA silencing-based approach has been successfully employed as a potential tool for disease resistance in crop plants. This chapter deals with the production and status of transgenic plants engineered for disease resistance. © 2025 Apple Academic Press, Inc. All rights reserved.