Browsing by Author "Veeresh Dube"

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Negatively charged liposomes of sertraline hydrochloride: Formulation, characterization and pharmacokinetic studies
(Editions de Sante, 2020) Tejpratap Chauhan; Varsha Rani; Bhupendra Sahu; Adity Sharma; Subhash Chand Kheruka; Sanjay Gambhir; Veeresh Dube; Lalit M. Aggarwal; Ruchi Chawla
The present study was carried out with an objective to study the extent of delivery of negatively charged liposomes of sertraline hydrochloride to brain via intravenous route for treatment of depressive –like symptoms. Liposomes of sertraline hydrochloride were formulated by film hydration technique using cholesterol, hydrogenated soya phosphatidylcholine-L-α-phosphatidylcholine, and distearoyl phosphatidyl glycerol sodium. Uniform sized vesicles with porous surface morphology with vesicle size of 151.59 nm were prepared. Radioactive imaging performed using 99mTcO4 showed ~5% of uptake of labelled liposomes in brain within 2 h of administration. On administration of liposomes and free drug suspension, approximately, 205.06 ng/ml and 87.18 ng/ml of sertraline were estimated in brain at 36 h. The liposomes can be transported by transcelluar transport which includes phagocytosis and the use of phosphatidylcholine enhances macrophage internalization and delivery to brain. The study indicated significantly higher concentration of sertraline in brain after 36 h, on administration of liposomes as compared to free sertraline suspension. © 2020 Elsevier B.V.
Polylactide-co-glycolide nanoparticles of antitubercular drugs: Formulation, characterization and biodistribution studies
(Future Science Ltd, 2014) Ruchi Chawla; Harshendra S. Solanki; Subhash Chand Kheruka; Sanjay Gambhir; Veeresh Dube; Lalit M. Aggarwal; Brahmeshwar Mishra
Background: The present study was designed to prepare and characterize poly lactide-co-glycolide nanoparticles of antitubercular drugs (ATDs) for delivery through oral route to alveolar macrophages. Methods: Nanoparticles were prepared by double emulsification solvent evaporation method. Ex vivo and in vivo drug accumulation studies were performed in alveolar macrophages, harvested by broncheoalveolar lavaging. Internalization of nanoparticles was studied by confocal laser scanning microscopy. γ-scintigraphy imaging using technetium-99m was done to study the biodistribution pattern of nanoparticles. Results: High intracellular concentrations of ATDs were observed in macrophages within 30 min of administration of nanoparticles. Intense radioactivity recorded in liver, spleen and lungs revealed uptake of nanoparticles in macrophages, abundantly present in mononuclear phagocyte system present in these organs. Conclusion: Targeted delivery of ATDs will help reduce dose and associated side effects including hepatotoxicity of ATDs. Further studies are required to assess the potential therapeutic advantages for treatment of TB. © 2014 Future Science Ltd.