Browsing by Author "Vibha Chauhan"

Filter results by typing the first few letters
Now showing 1 - 1 of 1
  • Thumbnail Image
    PublicationArticle
    Asp 187 and Phe 190 residues in lethal factor are required for the expression of anthrax lethal toxin activity
    (2002) Aparna Singh; Vibha Chauhan; Ajit Sodhi; Rakesh Bhatnagar
    Anthrax toxin consists of three proteins, protective antigen, lethal factor, and edema factor. Protective antigen translocates lethal factor and edema factor to the cytosol of mammalian cells. The amino-termini of lethal factor and edema factor have several homologous stretches. These regions are presumably involved in binding to protective antigen. In the present study we have determined the role of one such homologous stretch in lethal factor. Residues 187AspLeuLeuPhe190 were replaced by alanine. Asp187Ala and Phe190Ala were found to be non-toxic in combination with protective antigen. Their protective antigen-binding ability was drastically reduced. We propose that Asp187 and Phe190 are crucial for the expression of anthrax lethal toxin activity. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
An Initiative by BHU – Central Library
Powered by Dspace