Browsing by Author "Vikas"

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Chitosan nanoplatform for the co-delivery of palbociclib and ultra-small magnesium nanoclusters: dual receptor targeting, therapy and imaging
(Ivyspring International Publisher, 2024) Abhishesh Kumar Mehata; Virendra Singh; Vikas; Prachi Srivastava; Biplob Koch; Manoj Kumar; Madaswamy S. Muthu
Theranostic nanoparticles have gained significant attention in cancer diagnosis and therapy. In this study, estrone (ES) and folic acid (FA) functionalized single and dual receptor targeted theranostic chitosan nanoparticles were developed for breast cancer imaging and therapy. These nanoparticles (NPs) were loaded with palbociclib (PB) and ultra-small magnesium nanoclusters (UMN). The developed nontargeted theranostic NPs (PB-UMN-CS-NPs), estrogen receptor targeted theranostic NPs (PB-UMN-CS-ES-NPs), folate receptor targeted theranostic NPs (PB-UMN-CS-FA-NPs), and dual targeted theranostic NPs (PB-UMN-CS-ES-FA-NPs) have particle sizes of 178.4 ± 1.21 nm, 181.6± 1.35 nm, 185.1± 1.33 nm, and 198.2± 1.43 nm with surface charges of +19.02± 0.382 mV, +13.89±0.410 mV, +16.72±0.527 mV and +15.23±0.377 mV, respectively. Cytotoxicity studies on estrogen receptor (ER) and folate receptor (FR) expressing breast cancer cells revealed that dual-targeted theranostic NPs (PB-UMN-CS-FA-ES-NPs) were more effective, inhibiting cell growth by 54.17 and 42.23 times in MCF-7 and T-47D cells compared to free PB, respectively. Additionally, developed NPs were capable of inhibiting the cell cycle progression of MCF-7 cells from the G1 phase to the S phase more efficiently compared to free PB. Ultrasound and photoacoustic (USG/PA) imaging demonstrated that dual targeted theranostic NPs were capable of effectively reducing hypoxic tumor volume and significantly suppressing tumor vascularity compared to free PB, nontargeted, FR targeted and ER targeted NPs. Moreover, in vivo optical imaging demonstrated tumor specific accumulation of the dual-targeted theranostic NPs. Furthermore, in vitro hemocompatibility and histopathological studies confirmed the biocompatibility of developed nanoformulations. © The author(s).
Corrigendum to “Nanofibers of N,N,N-trimethyl chitosan capped bimetallic nanoparticles: Preparation, characterization, wound dressing and in vivo treatment of MDR microbial infection and tracking by optical and photoacoustic imaging” [Int. J. Biol. Macromol. 263 (2024) 130154](S0141813024009577)(10.1016/j.ijbiomac.2024.130154)
(Elsevier B.V., 2024) Ankit Kumar Malik; Chandrashekhar Singh; Abhishesh Kumar Mehata; Vikas; Aseem Setia; Madaswamy S. Muthu; Punit Tiwari; Dipti Verma; Ashim Mukherjee
The authors regret a mistake, which was entirely unintentional and occurred during the final stages of figure preparation. Specifically, during the data consolidation and visualization process, due to a labelling mistake in our repository of the same animal group, the 7B Untreated Day 4 images were mistakenly over-copied in the 7B Untreated Day 12 folder and the experimental results for Untreated Day 12 was inadvertently selected. Despite our thorough review processes though typically rigorous, we failed to catch this error by our naked eyes due to the compressed timeframe and multiple concurrent reviews. We have prepared a corrected version of Fig. 7B Untreated Day 12 which accurately reflects our findings and data discussed in the article, whereas the 7B Untreated Day 4 data is correct.[Formula presented] Fig. 7: (B) Skin histological studied under brightfield microscope (at 100× magnification) 4th day, 8th day and 12th day. The authors would like to apologise for any inconvenience caused. © 2024 Elsevier B.V.
Design of novel bioadhesive chitosan film loaded with bimetallic gold-silver nanoparticles for antibiofilm and wound healing activity
(Institute of Physics, 2023) Chandrashekhar Singh; Abhishesh Kumar Mehata; Vikas; Punit Tiwari; Aseem Setia; Ankit Kumar Malik; Sanjeev K Singh; Ragini Tilak; Madaswamy S Muthu
Microbial infections and antibiotic resistance are among the leading causes of morbidity and mortality worldwide. The bimetallic chitosan (CS)-capped gold-silver nanoparticles (CS-AuAg-NPs) were prepared by the seeded growth synthesis technique. The nanoparticles were optimized for particle size (PS), zeta potential (ZP) and antibacterial activity by Box-Behnken design at three levels and three factors. The developed CS-AuAg-NPs were polydispersed with mean hydrodynamic PS in the range of 55 - 289 nm and ZP ranges from +8.53 mV to +38.6 mV. The optimized CS-AuAg-NPs found to have a minimum inhibitory concentration and minimal bactericidal concentration of 1.625 ± 0.68 and 3.25 ± 0.74 µg ml−1 towards multidrug resistant (MDR) Staphylococcus aureus ATCC 25923 (MDR AT) and 3.25 ± 0.93 and 3.25 ± 0.86 µg ml−1 towards MDR S. aureus clinical isolate MDR1695 (MDR CI) strain, respectively. The CS-AuAg-NPs were much more effective against MDR AT and MDR CI compared to clindamycin standard. The live/dead assay of clinical isolates strain demonstrated significant reduction of bacterial cells ∼67.52 folds compared to control group in 12 h. The hemolysis study suggested that CS-AuAg-NPs were non-hemolytic and safer for application in the wound. Furthermore, CS-AuAg-NPs were distributed in the CS film, which showed 87% wound recovery after 7 d in mice model. Hence, we concluded that CS-AuAg-NPs was safer and more effective against MDR bacteria and capable of skin regeneration in the infected wound. © 2023 IOP Publishing Ltd
Efficient delivery of abciximab using mesoporous silica nanoparticles: In-vitro assessment for targeted and improved antithrombotic activity
(Elsevier B.V., 2022) Vishnu Priya; Vikas; Abhishesh Kumar Mehata; Dharmendra Jain; Sanjeev K. Singh; Madaswamy S. Muthu
Abciximab (ABX) is a chimeric monoclonal antibody reported for antithrombotic activity but their delivery remains challenging due to its poor stability in a biological system. The purpose of this research was to deliver ABX on the target efficiently using mesoporous silica nanoparticles (MSN). ABX coated mesoporous silica nanoparticles (MSN-ABX) were formulated and analyzed for particle size, shape, zeta-potential, surface morphology and surface chemistry. XPS analysis confirmed the presence of ABX on the surface of amino functionalized mesoporous silica nanoparticles (MSN-NH2). The degree of ABX attachment was 67.53 ± 5.81 % which was demonstrated by the Bradford assay. Furthermore, the targeting efficiency of the targeted nanoparticles has been evaluated by capturing the fluorescent images in-vitro which showed the significant accumulation of the ABX coated nanoparticles towards activated platelets. The significant (P < 0.05) increase in affinity of DiD dye loaded nanoparticles towards the activated platelets was confirmed by using an in-vitro imaging through photon imager optima. The hemolysis study of the nanoparticle formulations revealed that they were non-hemolytic for healthy human blood. The in-vitro antithrombotic effects of MSN-ABX were observed by blood clot assay which revealed its superior antithrombotic activity over clinical injection of ABX and could be a promising carrier for improved ABX targeted delivery. © 2022 Elsevier B.V.
Nanofibers of N,N,N-trimethyl chitosan capped bimetallic nanoparticles: Preparation, characterization, wound dressing and in vivo treatment of MDR microbial infection and tracking by optical and photoacoustic imaging
(Elsevier B.V., 2024) Ankit Kumar Malik; Chandrashekhar Singh; Punit Tiwari; Dipti Verma; Abhishesh Kumar Mehata; Vikas; Aseem Setia; Ashim Mukherjee; Madaswamy S. Muthu
Recent advancements in wound care have led to the development of interactive wound dressings utilizing nanotechnology, aimed at enhancing healing and combating bacterial infections while adhering to established protocols. Our novel wound dressings consist of N,N,N-trimethyl chitosan capped gold‑silver nanoparticles (Au-Ag-TMC-NPs), with a mean size of 108.3 ± 8.4 nm and a zeta potential of +54.4 ± 1.8 mV. These optimized nanoparticles exhibit potent antibacterial and antifungal properties, with minimum inhibitory concentrations ranging from 0.390 μg ml−1 to 3.125 μg ml−1 and also exhibited promising zones of inhibition against multi-drug resistant strains of S. aureus, E. coli, P. aeruginosa, and C. albicans. Microbial transmission electron microscopy reveals substantial damage to cell walls and DNA condensation post-treatment. Furthermore, the nanoparticles demonstrate remarkable inhibition of microbial efflux pumps and are non-hemolytic in human blood. Incorporated into polyvinyl alcohol/chitosan nanofibers, they form Au-Ag-TMC-NPs-NFs with diameters of 100–350 nm, facilitating efficient antimicrobial wound dressing. In vivo studies on MDR microbial-infected wounds in mice showed 99.34 % wound healing rate within 12 days, corroborated by analyses of wound marker protein expression levels and advanced imaging techniques such as ultrasound/photoacoustic imaging, providing real-time visualization and blood flow assessment for a comprehensive understanding of the dynamic wound healing processes. © 2024 Elsevier B.V.