Browsing by Author "Vinay Sharma"

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AIE active piperazine appended naphthalimide-BODIPYs: photophysical properties and applications in live cell lysosomal tracking
(Royal Society of Chemistry, 2019) Bhupendra Kumar Dwivedi; Roop Shikha Singh; Afsar Ali; Vinay Sharma; Shaikh M. Mobin; Daya Shankar Pandey
Piperazine appended naphthalimide-BODIPYs (NPB1-NPB4) exhibiting solvatochromism and aggregation-induced emission with a large Stokes shift (up to 146 nm) have been described. Separation of naphthalimide and BODIPY fluorophores by piperazine in these conjugates creates a donor-acceptor system and induces twisted intramolecular charge transfer, in addition to photoinduced electron transfer. The crucial role of naphthalimide, the alkyl chain length, the piperazine ring, and the solid-state packing on AIE has been extensively investigated by various studies. Superior cell permeability coupled with bio-compatibility of these conjugates offers a unique opportunity for their potential applications in live cell lysosomal tracking. © The Royal Society of Chemistry.
Anticancer Activity of Iridium(III) Complexes Based on a Pyrazole-Appended Quinoline-Based BODIPY
(American Chemical Society, 2017) Rajendra Prasad Paitandi; Sujay Mukhopadhyay; Roop Shikha Singh; Vinay Sharma; Shaikh M. Mobin; Daya Shankar Pandey
A pyrazole-appended quinoline-based 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (L1, BODIPY) has been synthesized and used as a ligand for the preparation of iridium(III) complexes [Ir(phpy)2(L1)]PF6 (1; phpy = 2-phenylpyridine) and [(η5-C5Me5)Ir(L1)Cl]PF6 (2). The ligand L1 and complexes 1 and 2 have been meticulously characterized by elemental analyses and spectral studies (IR, electrospray ionization mass spectrometry, 1H and 13C NMR, UV/vis, fluorescence) and their structures explicitly authenticated by single-crystal X-ray analyses. UV/vis, fluorescence, and circular dichroism studies showed that complexes strongly bind with calf-thymus DNA and bovine serum albumin. Molecular docking studies clearly illustrated binding through DNA minor grooves via van der Waals forces and their electrostatic interaction and occurrence in the hydrophobic cavity of protein (subdomain IIA). Cytotoxicity, morphological changes, and apoptosis have been explored by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and Hoechst 33342 staining. IC50 values for complexes (1, 30 μM; 2, 50 μM) at 24 h toward the human cervical cancer cell line (HeLa) are as good as that of cisplatin (21.6 μM) under analogous conditions, and their ability to kill cancer cells lies in the order 1 > 2. Because of the inherent emissive nature of the BODIPY moiety, these are apt for intracellular visualization at low concentration and may find potential applications in cellular imaging and behave as a theranostic agent. © 2017 American Chemical Society.
Carotenoids and pH of the culture medium play an important role in displaying metal stress in batch and semi-continuous cultures of Anabaena doliolum
(2009) Bhumi Nath Tripathi; Rohini Kasana; Vijeta Singh; Indu Bhatt; Ashutosh Singh; Vinay Sharma; Jai Prakash Gaur
We analysed the responses of Anabaena doliolum to elevated levels of copper and zinc in batch and semi-continuous cultures. Approximately 10, 4 and 8 and 5-times greater inhibition in final yield of A. doliolum occurred at 1, 2 μM Cu and 2.5 and 5 μM of Zn, respectively, in semi-continuous culture in comparison to batch culture. Protein, chlorophyl a and carotenoid contents of A. doliolum showed significantly (P<0.05) higher inhibition by test metals in semi-continuous culture than in batch culture. The greater sensitivity of different parameters of the test organism was related to the high metal content of the cells grown in semi-continuous system. Moreover, enhancement of pH of the culture suspension in batch culture showed a negative relationship with metal accumulation, and therefore with toxicity. This was due to decrease in free ionic concentrations of test metals. Carotenoids acted as a metal detoxifying agent by minimizing metal-induced inhibition in batch culture as was evident from its negative relationship with metal toxicity. © EDP Sciences, 2009.
Development and evaluation of near-isogenic lines for major blast resistance gene(s) in Basmati rice
(Springer Verlag, 2015) Apurva Khanna; Vinay Sharma; Ranjith K. Ellur; Asif B. Shikari; S. Gopala Krishnan; U.D. Singh; G. Prakash; T.R. Sharma; Rajeev Rathour; Mukund Variar; S.K. Prashanthi; M. Nagarajan; K.K. Vinod; Prolay K. Bhowmick; N.K. Singh; K.V. Prabhu; B.D. Singh; Ashok K. Singh
Key message: A set of NILs carrying major blast resistance genes in a Basmati rice variety has been developed. Also, the efficacy of pyramids over monogenic NILs against rice blast pathogenMagnaporthe oryzaehas been demonstrated. Abstract: Productivity and quality of Basmati rice is severely affected by rice blast disease. Major genes and QTLs conferring resistance to blast have been reported only in non-Basmati rice germplasm. Here, we report incorporation of seven blast resistance genes from the donor lines DHMASQ164-2a (Pi54, Pi1, Pita), IRBLz5-CA (Pi2), IRBLb-B (Pib), IRBL5-M (Pi5) and IRBL9-W (Pi9) into the genetic background of an elite Basmati rice variety Pusa Basmati 1 (PB1). A total of 36 near-isogenic lines (NILs) comprising of 14 monogenic, 16 two-gene pyramids and six three-gene pyramids were developed through marker-assisted backcross breeding (MABB). Foreground, recombinant and background selection was used to identify the plants with target gene(s), minimize the linkage drag and increase the recurrent parent genome (RPG) recovery (93.5–98.6 %), respectively, in the NILs. Comparative analysis performed using 50,051 SNPs and 500 SSR markers revealed that the SNPs provided better insight into the RPG recovery. Most of the monogenic NILs showed comparable performance in yield and quality, concomitantly, Pusa1637-18-7-6-20 (Pi9), was significantly superior in yield and stable across four different environments as compared to recurrent parent (RP) PB1. Further, among the pyramids, Pusa1930-12-6 (Pi2+Pi5) showed significantly higher yield and Pusa1633-7-8-53-6-8 (Pi54+Pi1+Pita) was superior in cooking quality as compared to RP PB1. The NILs carrying gene Pi9 were found to be the most effective against the concoction of virulent races predominant in the hotspot locations for blast disease. Conversely, when analyzed under artificial inoculation, three-gene pyramids expressed enhanced resistance as compared to the two-gene and monogenic NILs. © 2015, Springer-Verlag Berlin Heidelberg.
Linkage analysis for drought tolerance in kharif rice of assam using microsatellite markers
(National Institute of Science Communication and Information Resources (NISCAIR), 2019) J.P. Sahoo; Vinay Sharma; Rahul K. Verma; S.K. Chetia; A.R. Baruah; M.K. Modi; Vinod Kumar Yadav
Drought stress in rainfed ecosystem significantly limits the production of Ranjit, the most predominant high yielding rice variety of Assam. A mapping population comprising 85 F 4 individuals between Ranjit and a drought tolerant cultivar, ARC10372 was developed and genotyped with 80 microsatellite markers in order to understand the genetic basis of drought tolerance. The linkage map constructed based on a framework linkage map using these markers showed that the marker loci were distributed across 12 chromosomes spanning a distance of 273.4 cM with an average interval of 3.41 cM between marker loci. Most of the marker loci were found to be in good fit with the expected Mendelian segregation ratio; however, thirteen marker loci in total showed segregation distortion on six chromosomes. The linkage map generated in the study will facilitate mapping of quantitative trait loci imparting drought tolerance in rice of Assam and their map-based cloning. © 2019, National Institute of Science Communication and Information Resources (NISCAIR). All rights reserved.
Mandibular conservation in oral cancer
(2012) Latha P. Rao; Mridula Shukla; Vinay Sharma; Manoj Pandey
Surgery is one of the established modes of initial definitive treatment for a majority of oral cancers. Invasion of bony or cartilaginous structures by advanced upper aero-digestive tract cancer has been considered an indication for primary surgery on the basis of historic experience of poor responsiveness to radiation therapy [1]. The mandible is a key structure both in the pathology of intra-oral tumours and their surgical management. It bars easy surgical access to the oral cavity, yet maintaining its integrity is vital for function and cosmesis. Management of tumours that involve or abut the mandible requires specific understanding of the pattern of spread and routes of tumour invasion into the mandible. This facilitates the employment of mandibular sparing approaches like marginal mandibulectomy and mandibulotomy, as opposed to segmental or hemimandibulectomy which causes severe functional problems, as the mandibular continuity is lost. Accurate preoperative assessment that combines clinical examination and imaging along with the understanding of the pattern of spread and routes of invasion is essential in deciding the appropriate level and extent of mandibular resection in oral squamous cell carcinoma. Studies have shown that local control rates achieved with marginal mandibulectomy are comparable with that of segmental mandibulectomy. In carefully selected patients, marginal mandibulectomy is an oncologically safe procedure to achieve good local control and provides a better quality of life. This article aims to review the mechanism of spread, evaluation and prognosis of mandibular invasion, various techniques and role of mandibular conservation in oral squamous cell carcinoma. © 2010 Elsevier Ltd. All rights reserved.
Mechanism of Cu- and Cd-Induced Proline Hyperaccumulation in Triticum aestivum (Wheat)
(2013) Bhumi Nath Tripathi; Vijeta Singh; Bunichi Ezaki; Vinay Sharma; J.P. Gaur
Proline hyperaccumulation, a frequently reported phenomenon in the plant kingdom, is considered an adaptive strategy of plants to cope with a variety of stresses. The present study demonstrated the mechanism of proline hyperaccumulation in Triticum aestivum exposed to excess Cu and Cd. The intracellular level of proline was increased significantly in the plants treated with test metals. Enhanced proline accumulation due to Cu and Cd was negatively correlated with the growth of the treated plants, indicating that plants divert their resources toward induction of survival strategies rather than improving growth and development. The activities of pyrroline-5-carboxylate synthetase (P5CS) and pyrroline-5-carboxylate reductase (P5CR) were increased immediately after metal treatment and remained higher through the end of the experiment, whereas ornithine amino transferase (OAT) activity of the treated plants was lower than P5CS and P5CR enzymes. The activity of proline dehydrogenase (PDH) was decreased sharply in the early phase of metal exposure (up to 12 h) but remained unchanged thereafter until the end of the experiment. Expression analysis of the transcripts of P5CS, OAT, and PDH also supports the above findings and indicates the tissue-specific expression of these genes. Based on these results, increased proline synthesis through the glutamate pathway involving the P5CS and P5CR enzymes is a major contributor to proline hyperaccumulation followed by decreased catabolism of proline. © 2013 Springer Science+Business Media New York.
Pyrazole appended quinoline-BODIPY based arene ruthenium complexes: their anticancer activity and potential applications in cellular imaging
(Royal Society of Chemistry, 2018) Rajendra Prasad Paitandi; Vinay Sharma; Vishwa Deepak Singh; Bhupendra Kumar Dwivedi; Shaikh M. Mobin; Daya Shankar Pandey
Synthesis of an entirely new series of arene ruthenium complexes [Ru(η6-C6H6)(L1)Cl]PF6, (1), [Ru(η6-C10H14)(L1)Cl]PF6 (2), [Ru(η6-C6H6)(L2)Cl]PF6 (3) and [Ru(η6-C10H14)(L2)Cl]PF6 (4) involving 5-[2-(1H-pyrazol-1-yl)quinoline]-BODIPY (L1) and 5-[6-methoxy-2-(1H-pyrazol-1-yl)quinoline]-BODIPY (L2) was described. The ligands and complexes were thoroughly characterized by various physicochemical techniques and the structures of L1, 1 and 4 were determined by X-ray single crystal analyses. Photo-/ and electrochemical property, DNA binding, cytotoxicity, cellular uptake and apoptotic studies on 1-4 were performed by various methods, while singlet oxygen-mediated cytotoxicity via photo-irradiation by visible light was supported by 1,3-diphenylisobenzofuran titration studies. Binding of the complexes in the minor groove of CT-DNA via van der Waals forces and electrostatic interactions was affirmed by molecular docking studies. In vitro antiproliferative activity and photocytotoxicity of 1-4 were examined against the human cervical cancer cell line (HeLa) which clearly showed that these are extremely photocytotoxic under visible light (400-700 nm, 10 J cm−2; IC50 49.15, 1; 25.18, 2; 15.85, 3; 12.87, 4), less toxic in the dark (IC50 > 100 μM) and preferentially accumulate in the lysosome of the HeLa cells. Further, these complexes behave as a potential theranostic agent and their ability to kill cancer cells under visible light lies in the order 4 > 3 > 2 > 1. © The Royal Society of Chemistry.
Recent developments of molecular/biosensor diagnostics for SARS-CoV-2 detection
(Elsevier, 2022) Rupesh K. Mishra; Navin Pai; K. Yugender Goud; Ravi Ranjan Kumar Niraj; Deepak Kumar; Gaurang Patel; Vinod Singh Gour; Amin Zareei; Rahim Rahimi; Vinay Sharma; Roger Narayan; Lia Stanciu
The WHO deemed on March 11, 2020 that coronavirus disease 2019 (COVID-19) is a global pandemic. Per available data on October 6, 2020, there have been 35, 347, 404 confirmed cases of COVID-19, including 1, 039, 406 deaths; however, even this staggering figure is likely to be an underestimate (WHO dashboard). The COVID-19 pandemic has strained national health systems, leading to a health catastrophe and imposing an extraordinary socioeconomic burden. The WHO has set forth a series of targeted immediate actions that countries can take to maintain access to essential health services. The effort to scale COVID-19 testing by orders of magnitude may be facilitated through the use of several molecular tools. © 2022 Elsevier Inc. All rights reserved.