Browsing by Author "Vindhya Mohindra"

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Derivation and characterization of a ES-Like cell line from Indian catfish heteropneustes fossilis blastulas
(2014) Anindya S. Barman; Kuldeep K. Lal; Gaurav Rathore; Vindhya Mohindra; Rajeev K. Singh; Akankaha Singh; Praveen Khare; Bechan Lal
A cell line designated as HFB-ES was established from blastula stage embryos of H. fossilis (Singhi). The embryonic cells were harvested and maintained in Leibovitz's medium supplemented with 15% fetal bovine serum. The cell line had been subcultured for more than 90 passages in a period of 24 months. HFB-ES cells were able to grow at temperatures between 25 and 35°C with an optimum temperature of 28°C. The growth rate of HFB-ES was proportional to FBS concentration, with optimum growth seen at 15% FBS concentration. The originality of the cell line was confirmed by sequencing of cytochrome oxidase c subunit I (COI), cytochrome b gene, and microsatellite DNA profile. Results of chromosome complements of HFB showed normal karyo-morphology with 56 (2 n) diploid number of chromosomes after 40 passages which indicated that the developed cell line is chromosomally stable. The pluripotency of HFB was demonstrated by alkaline phosphatase activity and Oct-4 gene expression. Expression of GFP reporter gene was successful in HFB-ES. These results indicated that HFB-ES could be utilized for future gene expression studies. © 2014 Anindya S. Barman et al.
Effect of extender composition on sperm cryopreservation of Asian catfish Heteropneustes fossilis (Bloch) and Clarias Batrachus (linnaeus)
(Asian Fisheries Society, 2009) Kuldeep K. Lal; Anindya S. Barman; Peyush Punia; Praveen Khare; Vindhya Mohindra; Bechan Lal; A. Gopalakrishnan; Rama. S. Sah; Wazir S. Lakra
Air breathing catfish species Heteropneustes fossilis (Bloch) and Clarias batrachus (Linn.) are important table fish and fetch high market price. Cryopreservation of spermatozoa can be a useful tool in captive seed production for domestication and aquaculture of these catfish species. The objective of the present study was to identify optimum extender composition for sperm cryopreservation of the two species, H. fossilis and C. batrachus. Four extender compositions Hank’s Balanced Salt Solution (HBSS), Modified Hank’s Balanced Salt Solution (M-HBSS), Modified Hank’s Balanced Salt Solution with hen’s egg yolk (M-HBSS with EY) and European catfish were evaluated for cryopreservation of catfish sperm and 10 % Dimethyle Sulphoxide (DMSO) was used as a cryoprotectant. The pooled milt exhibiting 70-80% motile sperm was used for cryopreservation experiment. After storage for 48 hrs at -196ºC, the milt was thawed and evaluated for fertility test. The percentage of hatching was used as a parameter for the comparative evaluation of different extender composition. In H. fossilis extender M-HBSS indicated highest hatching rate (49.06%), followed by HBSS (42.76%), M-HBSS with EY (37.46%) and European catfish (29.47%). The hatching success with extender M-HBSS did not differ significantly (P > 0.05) from the control group (51%). In C. batrachus extender HBSS exhibited highest hatching (62.1 %), followed by M-HBSS with EY (51.6%), European Catfish (46.3%) and M-HBSS (40.9%). The hatching rate in control was 90% in C. batrachus. The results indicated that the two species differ in the protocol for sperm cryopreservation. The paper presents successful cryopreservation of sperm with the production of viable hatchlings of H. fossilis and C. batrachus for the first time. The protocol reported in the study can be used for scaling up of seed production of these two catfish species. © 2009, Asian Fisheries Society. All rights reserved.
Optimisation of sperm cryopreservation protocol for Asian stinging catfish Heteropneustes fossilis (Bloch, 1794), to support hatchery seed production
(Indian Council of Agricultural Research, 2023) Kuldeep K. Lal; Anindya S. Barman; Peyush Punia; Praveen Khare; Vindhya Mohindra; Rajeev K. Singh; Rama S. Sah; Sullip K. Majhi; Bechan Lal
The present study experimentally evaluated different parameters such as extender composition, storage duration and egg to sperm ratio to optimise parameters for sperm cryopreservation for in Asian stinging catfish Heteropneustes fossilis. Four extender compositions viz. Hanks Balanced Salt Solution (HBSS), Modified HBSS (M-HBSS), Modified HBSS with hen’s egg yolk (M-HBSS with EY) and European catfish extender were evaluated for sperm cryopreservation with dimethyl sulphoxide (DMSO) as cryoprotectant (10% v/v). The hatching rate was used as standard to evaluate the fertility of thawed sperm at different periods of storage (2, 270 and 668 days). After 2 days, extender M-HBSS exhibited highest hatching rate (49.06±2.67%) which was comparable to the control value (51.00±7.23%), followed by HBSS (42.76±32.0%), M-HBSS with EY (37.46±24.34%) and European catfish extender (29.47±25.61%), respectively. After 270 days with M-HBSS, hatching rate was 64.34±8.84% similar to the control; however, cryopreserved sperm with other extenders exhibited lower hatching rate as compared to control and M-HBSS. After 668 days, sperm cryopreserved in the extender M-HBSS, only yielded successful hatching. Results indicated that presence of egg yolk in the extender M-HBSS did not improve the performance of cryopreserved sperm. The successful hatching using cryopreserved sperm after 270 and 668 days of storage indicates M-HBSS as a useful extender for sperm cryobanking and seed production of H. fossilis. © 2023 Indian Council of Agricultural Research.