Browsing by Author "Vivek Sahajpal"

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Assessment of significance and forensic relevance of SE33 (ACTBP2) locus in five Indian populations
(Elsevier Inc., 2021) Shivani Dixit; Pankaj Shrivastava; Hirak Ranjan Dash; Kamlesh Kaitholia; Vivek Sahajpal; Shubhasish Sahoo; Varsha Srivastava; H. Surekha Rani; Aditi Mishra; Sumit Kumar Choudhary; Ajeesh Thekkatavan; Gyaneshwer Chaubey; R.K. Kumawat
The study was conducted to evaluate the genetic variability, forensic reliability and potential of SE33 STR marker in five different Indian populations. 1400 blood samples of randomly selected unrelated individuals from various regions of Odisha (508), Madhya Pradesh (370), Telangana (305), Himachal Pradesh (168) and Rajasthan (49) were genotyped and evaluated for allelic diversity and statistical parameters for SE33 marker in selected Indian populations along with its comparison from global populations. This evaluation uncovered the highest discrimination power in population of Portugal (0.995), India (0.994), Bahrain (0.994) and Colombia (0.994). However, the highest observed heterozygosity was reported in Malaysian population (0.953) out of other reported populations. In all the studied five Indian populations SE33 was found to be have the highest discrimination power and lowest probability of match which envisions its application in varied applications of forensic DNA analysis such as human identification, paternity disputes and mass disaster as well as other population genetic studies. © 2021 Elsevier Inc.
Developmental validation of the Ingenomics™ AutoProfiler STR system: a 6-dye STR multiplex kit for forensic DNA applications
(Springer, 2025) Shivkant Sharma; Rajendra V.E. Chilukuri; Rhea Shetkar; Ramkishan K. Kumawat; Vivek Sahajpal; Shivani Dixit; Braja Kishore Mohapatra; Dhirendra Singh Yadav; Kapil Dev; Rubina Mohammed; Umema Ahmad; Krishna Mani Yadav; Anita Pundir; Jaison Jeevan Sequeira; G. Chaubey
The Ingenomics™ AutoProfiler system utilises a 6-dye, multiplex genotyping technology that is optimised for the simultaneous amplification of 20 Combined DNA Index System (CODIS) short tandem repeat (STR) markers, along with D6S1043, Penta D, Penta E, DYS391, SE33, and two Y-Indels (Rs2032678, Rs771783753), as well as the Amelogenin loci. This kit includes specialised internal quality control (IQC) markers, both small and large, to evaluate the quality parameters for each sample. Designed for a diverse range of applications—such as forensic DNA casework, identity establishment, database management, kinship testing, scientific research, and other analyses related to human genetic identification—this multiplex system is particularly effective for analysing low-copy number (LCN) DNA from severely compromised forensic samples, as it also evaluates two SNP markers. The multidimensional validation study aligned with the guidelines set forth by the Scientific Working Group on DNA Analysis Methods (SWGDAM) examined crucial parameters, including PCR conditions, reproducibility, analytical threshold calculation, sensitivity, species specificity, stability, mixture analysis, casework sample analysis, direct amplification, and concordance. The optimal DNA concentration range was between 60 and 500 pg/µL, with an analytical threshold established at 50 relative fluorescence units (RFUs). Overall, the findings indicate that the Ingenomics™ AutoProfiler kit is a reliable, robust, and suitable assay for human identification in casework DNA analysis and database construction. © The Author(s) under exclusive licence to Archana Sharma Foundation of Calcutta 2025.
Molecular characterization and forensic relevance of the autosomal STRs for the population of North Indian geographical province Himachal Pradesh, India
(Elsevier B.V., 2021) Arun Sharma; Naresh Kumar; Ashwani Bhardwaj; Vivek Sahajpal; R.K. Kumawat; Shivani Dixit; Gyaneshwer Chaubey; Pankaj Shrivastava
Keeping in view the diverse demography of India, present study was undertaken to explore the molecular characterization and forensic relevance of 20 autosomal STRs for the highly diverse population of north Indian state Himachal Pradesh. 724 unrelated individuals from the admixed population of Himachal Pradesh were undertaken for present study and 20 autosomal STRs used to explore genomic diversity of studied population. A total of 270 different alleles along with 13.5 alleles per locus were observed. The allele 8 of the locus TPOX was observed as the most frequent allele. Observed heterozygosity ranged from 0.677 to 0.898, which supported wide range of selection of the unrelated individuals for this study. Combined power of discrimination, power of exclusion, matching probability and paternity index were observed as 1, 0.9999999958, 3.9 × 10−26 and 2.3 × 108 respectively, across the studied loci. In the population differentiation test, studied population showed genetic relatedness with Indian population rather than the populations of West, North and North east countries. Present study deciphered the novel autosomal STR data, which could be useful for the forensic application and population genetic studies. © 2021 Elsevier B.V.