Browsing by Author "Y.B. Tripathi"

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Anti-platelet activating factor property of Rubia cordifolia Linn.
(1993) Y.B. Tripathi; S. Pandey; S.D. Shukla
Rubia cordifolia is clinically used for the purification of blood by the physicians of the Indian System of Medicine. For the first time, the effect of the partially purified fraction of this whole plant has been studied on rabbit platelets. It inhibits the platelet aggregation induced by PAF (platelet activating factor) but not thrombin. It also inhibits the binding of 3H-PAF to the platelets in the dose-dependent manner. Thus it appears that R. cordifolia inhibits action of PAF at its receptor level either by it's blocking or by desensitization.
Antilipid peroxidative property of Nardostachys jatamanasi
(1996) Y.B. Tripathi; Ekta Tripathi; Anil Upadhyay
Antiperoxidative property of N. jatamanasi was tested by using iron induced lipid peroxidation in 5% rat liver homogenate. The degree of peroxidation was quantitated by thiobarbituric acid reactive substance (TBARS) content. The extract provided protection against lipid peroxidation. The hexane fraction was more potent than the alcoholic extract. Results suggested antilipid peroxidative property of the plant.
Antioxidant and anti-inflammatory property of Sandhika: A compound herbal drug
(1995) S. Chaurasia; P. Tripathi; Y.B. Tripathi
The present study was envisaged to assess the rationality for the use of 'Sandhika', a popular Ayurvedic drug in rheumatoid arthritis. This drug, when tested against carrageenan induced paw oedema and cotton pellet granuloma, showed significant anti-inflammatory activity at the dose of 0.25 g/kg body weight. The antioxidant property was assessed by determining cumene hydroperoxide (CHP) induced lipid peroxidation and reduced glutathione content in rat liver homogenate (in vitro). Experiments show the significant protection against lipid peroxidation at the dose of 80 μg/ml, measured as reduction in the level of malondialdehyde (MDA) induced by 1.5 mM cumene hydroperoxide (CHP). This effect was accompanied by the maintained reduced glutathione (GSH) content in drug treated rats. Oral treatment of drug up to 2 g/kg body weight for 15 days did not show any rise in serum transaminases (SGOT and SGPT). The results suggest that 'Sandhika' which is an indigenous drug for inflammation with no detectable adverse effect, might be acting through scavenging the free radicals.
Antioxidant property of Rubia cordifolia extract and its comparison with vitamin E and parabenzoquinone
(1995) Y.B. Tripathi; Savita Shukla; Mukta Sharma; V.K. Shukla
This report investigates the antioxidant properties of R. cordifolia extract for protection against lipid peroxidation and reduced glutathione (GSH) content in rat liver homogenate compared with vitamin E and parabenzoquinone (PBQ). R. cordifolia protects against depletion of glutathione in a dose and time dependent manner, an inverse correlation was observed between reduced glutathione depletion and malondialdehyde (MDA) formation. Interestingly, parabenzoquinone showed a better response than R. cordifolia on cumene hydroperoxide (CHP) induced malon‐dialdehyde (MDA) release but there was no response in the rate of reduced glutathione (GHS) depletion. Thus it appears that R. cordifolia is a potent antiperoxidative and antioxidant. The mechanism of action is different to that of vitamin E and parabenzoquinone (PBQ). Copyright © 1995 John Wiley & Sons, Ltd.
Antioxidant property of Smilex china Linn
(2001) Y.B. Tripathi; A.K. Upadhyay; P. Chaturvedi
Smilex china Linn. (Smilacaceae Syn Liliaceae) has special pharmacognostic feature as its root is multiseriate, with sclerenchymatic pericycle. The alcoholic extract of rhizome of S. china shows significant protection against FeSO4 induced lipid peroxidation in rat liver homogenate, but has no effect on the rate of oxidation of reduced glutathione. This fraction scavenges the superoxide and hydroxyl radicals, but the effect was more towards the removal of superoxide than that of hydroxyl radicals. Thus it could be concluded that rhizome of S. china has strong antioxidant property.
Assessment of endocrine response of Inula racemosa in relation to glucose homeostasis in rats.
(1995) Y.B. Tripathi; P. Chaturvedi
Alcoholic extract of the root of I. racemosa lowers blood glucose and enhances liver glycogen without increasing plasma insulin in rats. There is also no increase in degree of degranulation of the beta cells of pancreas. In similar conditions it does not show any effect on activity of adrenal gland. However, thyroid gland undergoes activation at the later stage (delayed response). It appears that hypoglycemic response of I. racemosa is not due to enhanced secretion/synthesis of insulin; the action may be at the peripheral level by potentiating the insulin sensitivity.
Assessment of the adrenergic beta-blocking activity of inula racemosa
(1988) Y.B. Tripathi; Pratibha Tripathi; B.N. Upadhyay
Inula racemosa root powder was investigated in patients with proven ischaemic heart disease. The powder prevented ST-segment depression and T-wave inversion as observed in the post-exercise electrocardiogram. The petroleum ether extract of roots lowered plasma insulin and glucose levels within 75 min of oral administration to albino rats and it significantly counteracted adrenaline-induced hyperglycaemia in rats. The extract further showed negative inotropic and negative chronotropic effects on frog heart. All these findings indicate that one of the constituents of Inula racemosa may have adrenergic beta-blocking activity. © 1988.
Ayurvedic supportive therapy in the management of breast cancer
(Elsevier GmbH, 2021) Manoj Kumar Dash; Namrata Joshi; D.N.S Gautam; Remya Jayakumar; Y.B. Tripathi
Introduction: Breast cancer is the second most common cancer among women. Initial signs of cancer start in the lymph nodes located under the arm, within the breast, and near the collarbone. Many promising remedies for breast cancer are included in Ayurveda, the Indian system of medicine. Nowadays, multi-targeted herbal drugs are used as an adjuvant therapy alongside conventional medicine in the management of t0068is type of cancer. The present review concentrates on the available literature found in Ayurveda regarding plants and breast cancer. Methods: Web of Science, PubMed, Google Scholar were searched, resulting in 115 articles, as per the authors study criteria, by using keywords, breast cancer, and medicinal plants, then were able to aggregate 64 therapeutic targets from 35 different plant species in this review article. Result: Seven treatment areas were identified: Anti-inflammatory, Immunomodulatory, Endocrine suppressor, Tumour suppressor, Glut-1 inhibitor, Aromatase inhibitor, and Lectin-containing plants. Among the molecular targets, 51 % (33/64) caused apoptosis and 37 % (24/64) demonstrated cell proliferation activity. The review provides evidence of data suggesting that 17 % therapeutic targets have carcinogen inactivation activity, 10 % have precancerous growth prevention activity, 9% have mutagenesis and gene expression activity, and 4 % regulate toxic metabolism. Conclusion: Ayurvedic herbs that suppress multiple pathways must be chosen for prevention as well as treatment of the disease. It may not only be beneficial in reducing the harms of chemotherapeutic agents but also enhances their efficacy as supportive care. © 2021 Elsevier GmbH
Choleretic action of andrographolide obtained from Andrographis paniculata in rats
(1991) G.S. Tripathi; Y.B. Tripathi
Andrographolide, a diterpene, isolated from Andrographis paniculata exhibited a strong choleratic action when administered intraperitoneally (i.p.) to albino rats. This substance induced an increase in bile flow together with a change in the physical properties of the bile secretion. Copyright © 1991 John Wiley & Sons, Ltd.
Effect of Asparagus racemosus rhizome (Shatavari) on mammary gland and genital organs of pregnant rat
(2005) S.K. Pandey; A. Sahay; R.S. Pandey; Y.B. Tripathi
Asparagus racemosus (AR) Willd (family Liliaceae) is commonly known as Shatavari. The alcoholic extract of its rhizome was administered orally to adult pregnant female albino rats at a dose of 30 mg/100 g body weight, daily for 15 days (days 1-15 of gestation). The macroscopic findings revealed a prominence of the mammary glands, a dilated vaginal opening and a transversely situated uterine horn in the treated group of animals. The weight of the uterine horns of the treated group was found to be significantly higher (p < 0.001) but the length was shorter (p > 0.01). Microscopic examination of the treated group showed proliferation in the lumen of the duct of mammary gland. It was obliterated due to hypertrophy of ductal and glandular cells. Hyperplasia of the glandular and muscular tissue and hypertrophy of the glandular cells were observed in the genital organs. The parenchyma of the genital organs showed abundant glycogen granules with dilated blood vessels and thickening of the epithelial lining. The oviduct in the treated group showed hypertrophied muscular wall, whereas the ovary revealed no effect of the drug. The results suggest an oestrogenic effect of Shatavari on the female mammary gland and genital organs. Copyright © 2005 John Wiley & Sons, Ltd.
Effect of Momordica charantia on lipid profile and oral glucose tolerance in diabetic rats
(2004) P. Chaturvedi; S. George; M. Milinganyo; Y.B. Tripathi
In this study, the methanol extract of Momordica charantia fruit extract was administered to diabetic rats to assess the long term effect of the extract on the lipid profile and the oral glucose tolerance test. Treatment for 30 days showed a significant decrease in triglyceride, low density lipoprotein and a significant increase in high density lipoprotein level. A significant effect on oral glucose tolerance was also noted. Chronic administration showed an improvement in the oral glucose tolerance curve. The effect was more pronounced when the test was done in rats fed the extract on the day of the test compared with tests done in rats which were not fed the extract on the same day. Copyright © 2004 John Wiley & Sons, Ltd.
Effect of Saussurea lappa alcoholic extract on different endocrine glands in relation to glucose metabolism in the rat
(1993) P. Chaturvedi; P. Tripathi; S. Pandey; U. Singh; Y.B. Tripathi
In this report the alcoholic extract of the root of Saussurea lappa C. B. Clarke (Compositae) has been used on albino rats to investigate the response on the histology of the thyroid gland, adrenal gland, pancreas and liver. Its effect on liver glycogen, blood glucose and plasma insulin has also been investigated. Treatment up to 7 days shows a significant hypoglycaemic response without an increase in plasma insulin. The acute response shows the accumulation of glycogen in the liver followed by its depletion. The histology of the thyroid shows stimulation. Liver sections do not show any toxic effects up to 400 mg/kg body weight. No change was observed in sections of adrenal gland and pancreas. Copyright © 1993 John Wiley & Sons, Ltd.
Effect of Semecarpus anacardium nuts on lipid peroxidation
(2001) Y.B. Tripathi; A.V. Singh
Alcoholic extract of pericarp showed significant protection against FeSO4 induced lipid peroxidation, as compared with whole native nut and seeds. Mechanism of action may be through metal chelation or activation of endogenous antioxidant enzymes, because the extract did not show hydroxyl and super oxide anion scavenging property. Further in vitro experiments against FeSO4, it did not maintain the level of reduced glutathione.
Effect of Strychnos nux vomica alcohol extract on lipid peroxidation in rat liver
(1996) Y.B. Tripathi; S. Chaursia
This report investigates the antioxidant properties of the purified Strychnos nux vomica L. seed extract for protection against non-enzymatic formation of lipid peroxides and on reduced glutathione (GSH) levels in rat liver homogenate. Results were compared with the natural antioxidant vitamin E. For the first time it could be reported that this drug protects against cumene hydroperoxide (CHP) induced lipid peroxidation in a dose dependent manner. It also inhibits the process of the lipid peroxidation, once induced. The drug significantly maintains the hepatic content of glutathione in a dose and time dependent mariner even in the presence of the above toxin (CHP). Thus it appears that S, nux vomica is a potent antioxidant and the mechanism of action could be through the scavenging of free radicals.
Evaluation of Chenopodium ambrosioides oil as a potential source of antifungal, antiaflatoxigenic and antioxidant activity
(2007) Rajesh Kumar; Ajay Kumar Mishra; N.K. Dubey; Y.B. Tripathi
Essential oil extracted from the leaves of Chenopodium ambrosioides Linn. (Chenopodiaceae) was tested against the aflatoxigenic strain of test fungus Aspergillus flavus Link. The oil completely inhibited the mycelial growth at 100 μg/ml. The oil exhibited broad fungitoxic spectrum against Aspergillus niger, Aspergillus fumigatus, Botryodiplodia theobromae, Fusarium oxysporum, Sclerotium rolfsii, Macrophomina phaseolina, Cladosporium cladosporioides, Helminthosporium oryzae and Pythium debaryanum at 100 μg/ml. The oil showed significant efficacy in inhibiting the aflatoxin B1 production by the aflatoxigenic strain of A. flavus. During in vivo investigation it protected stored wheat from different storage fungi for one year. Chenopodium oil also exhibited potent antioxidant activity when tested by ABTS method. All these observations suggest the possible exploitation of the Chenopodium oil as potential botanical fungitoxicant in ecofriendly control of post harvest biodeterioration of food commodities from storage fungi. © 2006 Elsevier B.V. All rights reserved.
Gene network analysis of Diabetic Nephropathy and its associated diseases- An in silico study
(World Research Association, 2022) P. Shree; N. Pandey; R. Chaube; N. Garg; Y.B. Tripathi
Metabolic syndrome (MetS) is a cluster of conditions including Diabetic nephropathy (DN), Hypertension (HT), Type 2 Diabetes mellitus in obese and obesity. Network biology has been developed as a way to review gene interactions. Herein an effort was made to identify common genes between the above MetS using Cytoscape3.0. Further molecular docking study has been performed to identify potential inhibitors for common genes using active phytoconstituents of Pueraria tuberosa (PTY-2). AGT (angiotensinogen) and ACE (angiotensinconverting enzyme) were found to be common genes between DN and HT. From the molecular docking study, tuberosin (9.19 kcal/mol) and robinin (11.28 kcal/mol) showed highest binding affinity with AGT and ACE and followed all drug-like properties as per Lipinski's rule of five and Admet prediction. Hence, based on our findings we may suggest that by inhibiting AGT and ACE, the downstream functioning pathways are responsible for these diseases and their associated complications can be normalized. © 2022 World Research Association. All rights reserved.
Hemp Usage as a Green Building Material, Plastic, and Fuel
(Springer International Publishing, 2022) N. Rajak; N. Pandey; Y.B. Tripathi; N. Garg
The growing apprehensions about increasing carbon emissions have made global leaders take robust action to prevent catastrophic climate change and global warming. Building construction is one of themany factors responsible for the increase in global pollution. Therefore, in this area, green construction is the most important step to reduce carbon emissions and other factors that cause the increased global pollution level. Hence, the revival of eco-friendly green materials could be a steppingstone in this way. Vegetal concrete construction materials based on biomass will be able to offer the beneficial solution of carbon sequestration in addition to the low embodied. These vegetal-based concretes are composed of an organic or inorganic binder and biomass derived from agroforestry industries such as rice husk, straw bale, hemp, kenaf, cork, and others. One of the vegetal concretes is hemp concrete, which has been extensively explored and researched, making it one of the best green materials for other purposes, such as an alternative to plastics and fuel in the form of biofuel. This chapter provides an overview of hemp-based concrete research, which is rapidly increasing in the green building sector, as well as plastic and fuel. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2022.
Hepatoprotective and toxicological evaluation of hepatomed. An ayurvedic drug
(1995) M. Sharma; P. Tripathi; V.P. Singh; Y.B. Tripathi
Hepatoprotective effect of Hepatomed (an ayurvedic drug containing water extract of 6 medicinal plants) has been studied on cumene hydroperoxide (CHP) induced lipid peroxidation and reduced glutathione content in rat liver homogenate. In vitro experiments show significant reduction in the level of nmalondialdehyde (MDA) induced by 1.5 mM cumene hydroperoxide (CHP). Glutathione content was almost maintained to normal in drug treated rats. Oral treatment of drug up to 3 ml/100 g body weight for 15 days did not show any rise in serum GOT and GPT. On similar doses, significant choleratic effect was observed without any adverse histological changes after 4 days treatment. The results suggest that 'Hepatomed' is a strong hepatoprotective ayurvedic medicine with no detectable adverse effects.
High-Resolution LCMS Profiling of Phenolic Compounds of Indian Black Carrot and Evaluation of Its Effect on Antioxidant Defense and Glucose Metabolism in Animal Model
(Springer, 2019) Tanmay Kumar Koley; Shivani Srivastava; Y.B. Tripathi; Kaushik Banerjee; Dasharath Oulkar; Arnab Goon; Ajay Tripathi; Bijendra Singh
Black carrot (Daucus carota L. ssp. sativus var. atrorubens Alef.), an indigenous vegetable of India, has been widely used for the preparation of traditional fermented product Kanji, and traditional medicine for treating various kinds of diseases. Although previous research has reported its biochemical composition and some in vitro functional properties, very scanty information is available to date on the in vivo effect of black carrot. Given this gap of knowledge, this study was aimed to investigate the effect of black carrot on antioxidant defense, glucose metabolism and renal, as well as hepatic toxicity in normal Charles Foster albino rats. In addition, secondary metabolites were identified by high-resolution LCMS and in vitro antioxidant activity was also assessed. LC/MS/MS analysis revealed the presence of nine anthocyanins and two flavonols. Among these, four new compounds were putatively identified for the first time. Under in vivo condition, feeding of black carrot juice for 30 days resulted in the reduction in the superoxide dismutase activity, both in dose- and time-dependent manner. It was also observed that feeding of rats with black carrot juice for 30 days reduced their blood triglyceride concentration, both in dose- and time-dependent manners. No significant effect was observed on plasma glucose concentration, and also, no toxicity was noted with regard to the functioning of the kidney and liver. © 2018, NAAS (National Academy of Agricultural Sciences).
Identification of therapeutic targets for controlling covid-19 pandemic by traditional system of ayurvedic medicines: A systematic review
(National Institute of Science Communication and Information Resources, 2020) M.K. Dash; N. Joshi; Y.B. Tripathi
COVID-19 is a severe respiratory disorder caused by the SARS COV-2 virus that involves limited innate immunity. Numerous publications have suggested that plants/minerals used in the traditional system of Ayurveda, has revealed much about the biology of COVID-19. One theory is that combination of anti viral, anti inflammatory, agents activating immune cells, herbs and metals may be helpful for severe acute respiratory syndrome coronavirus infection. Anti-viral drugs used for COVID-19 are those which block RNA synthesis and virus invasion, and bind to receptor proteins on the surface of cells, cell cycle protein, and physiological and pathological processes inhibitor. Anti-inflammatory drugs used for COVID-19 are those which controls transcription of DNA, cytokine production, break down the basement membrane, regulate outer mitochondrial membrane permeability, controlling the host cell life, stimulates activated B-cell and T-cell proliferation, virus dissemination, a slowdown of cell metabolism or secretion of cytokines. Drugs which is having role in the innate immunity, inhibits ROS, enhances cell lifespan, activates macrophages, physiological effects on cells activates the Lung resident immune cells. The focus of this review is to elucidate the Ayurvedic pharmacological properties with their therapeutic targets. © 2020, National Institute of Science Communication and Information Resources. All rights reserved.