Browsing by Author "Yamini Bhusan Tripathi"

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A multidisciplinary approach to standardize bhasmas (ayurvedic metallic preparations) [6]
(2006) Yamini Bhusan Tripathi
[No abstract available]
Active phytochemicals of Pueraria tuberosa for DPP-IV inhibition: In silico and experimental approach
(BioMed Central Ltd., 2017) Shivani Srivastava; Priya Shree; Yamini Bhusan Tripathi
Background: We had earlier reported that the extract of Pueraria tuberosa significantly inhibits DPP-IV enzyme, resulting in glucose tolerance response in rats. In this study, we have explored the active phytochemicals responsible for this potential. The results have been validated in both fasting and postprandial states in the plasma of normal rats and also in fasting blood and intestinal homogenates of diabetic models. Methods: Pueraria tuberosa water extract (PTWE) was administered to normal Charles Foster rats for 35days and to diabetic model (65mg/kg bw) for 10days. After treatments, oral glucose tolerance test (OGTT) and insulin was done for 90min, and the changes in the levels of GLP-1, GIP, and DPP-IV activities were monitored in fasting and postprandial states. In the case of the diabetic model, DPP-IV activity was measured in intestinal homogenate and basal insulin in plasma. The components of PTWE were analyzed via HPLC-MS based on their chemical formula, molecular mass, and retention time. Using the molecular docking study, we have selected the top five components having strong binding energy with DPP-IV. Results: The increase in secretion of GLP-1 and GIP was significantly higher in the postprandial state when compared to fasting condition. GLP-1 plasma concentration increased by 5.8 and 2.9 folds and GIP increased by 8.7 and 2.4 folds in PTWE and control rats, respectively. In contrast, the postprandial decrease in DPP-IV specific activities was recorded at 2.3 and 1.4 folds. The response in OGTT and insulin was also consistent with these changes. In comparison to diabetic controls, PTWE-administered rats showed decreased DPP-IV activity in the intestine, leading to enhanced basal insulin concentration. Through molecular docking, we found Puerarone and Robinin to be the most potential phytochemicals of PTWE for DPP-IV inhibition. Binding energy (kcal/mol) and dissociation constant (pM) of Robinin with DPP-IV protein were found to be 7.543 and 2,957,383.75, respectively. For Puerarone, it was 7.376 and 3,920,309, respectively. Conclusions: Thus, this study provides the novel active components that contribute to the DPP-IV inhibitory property of PTWE. © 2017 The Author(s).
Anti-oxidant, anti-apoptotic, anti-hypoxic and anti-inflammatory conditions induced by PTY-2 against STZ-induced stress in islets
(International Advancement Center for Medicine and Health Research Co., Ltd., 2019) Shivani Srivastava; Harsh Pandey; Surya Kumar Singh; Yamini Bhusan Tripathi
The earlier assessment of Pueraria tuberosa (PT) has shown anti-diabetic effects through enhancing incretin action and DPP-IV (Dipeptidyl peptidase-IV) inhibition. The aim of this work was to further explore the protective role of aqueous extract of Pueraria tuberosa tuber (PTY-2) against streptozotocin (STZ) induced islet stress in rats. Diabetes was induced by STZ (65 mg/kg body weight) in charles foster male rats. After 60 days of STZ administration, animals with blood glucose levels > 200 g/dL were considered as diabetic. All the rats were later divided into three groups: Group-1 (STZ untreated normal rats), Group-2 (Diabetic control), and Group-3 (PTY-2 [50 mg/100 g bw treatment for next 10 days to diabetic rats). The rats were then sacrificed after the 10th day of treatment accordingly. STZ treatment led to an increase in expression of Matrix metalloproteinases-9 (MMP-9), Tumour necrosis factor-α (Tnf-α), Hypoxia inducible factor-α (HIF-1α), Vascular endothelial growth factor (VEGF), Interleukin-6 (IL-6), Protein kinase C-ε (PKC-ε), Nuclear factor kappa-light-chainenhancer of activated B-cells (NFkB), and Caspase-3. Reverse Transcriptase-PCR (RT-PCR), Immunohistochemistry and Western-Blot analysis showed an increase in the expressions of Superoxide Dismutase (SOD) and Nephrin, and a decrease in the expressions of NFkB, PKC-ε, TNF-α, MMP-9, HIF-1α, VEGF, Caspase-3 and IL-6 after 10 days of PTY-2 treatment. The results showed that PTY-2 favorably changed all the expressions via anti-oxidant, antiapoptotic, anti-hypoxic and anti-inflammatory pathways, making itself as a protective agent against STZ induced islet stress. Further evaluation of PTY-2 might be helpful in establishing its role in the management of diabetes mellitus. © 2019, International Advancement Center for Medicine and Health Research Co., Ltd.
Better salary for university teachers having higher market value
(2005) Yamini Bhusan Tripathi
[No abstract available]
Challenges in marketing ayurvedic drugs in USA: Possible remedies
(2008) Yamini Bhusan Tripathi
[No abstract available]
Comparative in vitro antimicrobial and phytochemical evaluation of methanolic extract of root, stem and leaf of Jatropha curcas linn
(Pharmacognosy Network Worldwide, 2012) Amit Kumar Sharma; Mayank Gangwar; Ragini Tilak; Gopal Nath; Akhoury Sudhir Kumar Sinha; Yamini Bhusan Tripathi; Dharmendra Kumar
Background: Earlier researchers have reported antibacterial activity of different specific parts, but none of the reports show the comparative microbial and phytochemical studies of root, stem and leaf extract. Objective: To compare and investigate antimicrobial, qualitative phytochemical studies, phenol, flavonoid and TLC analysis of root, stem bark, leaf extracts of Jatropha curcas Linn family Euphorbiaceae. Methods: The dried plant powder was subjected to Soxhlet extraction with methanol. These solvent extracts were subjected to a preliminary phytochemical screening to detect the different chemical principles present viz., carbohydrates, proteins, amino acids, steroids, glycosides, alkaloids, flavonoids, tannins and phenolic compounds, fixed oils. Antimicrobial activity was evaluated by disc diffusion method and minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), minimum fungicidal concentration (MFC) was calculated by micro dilution method. Thin layer chromatography was also performed using solvent system chloroform, benzene, hexane, and ethyl acetate for the analysis of a number of constituents in the plant extract. The content of the total phenolics in the extract was determined spectrometrically according to the Folin-Ciocalteu procedure and calculated as catechol equivalent. The content of total flavonoids in the extract was determined and calculated as quercetin equivalent. Result: These extracts showed antibacterial, antifungal activities against gram-positive and gramnegative bacteria with varying magnitudes. The phytochemical analysis showed the presence of alkaloid, saponin, tanins, terpenoids, steroids, glycosides, phenols and flavonoids. Maximum phenolic content (38.8) was found in leaf extract and flavonoid content (18.14) in latex of plant. Discussion: It is concluded that the antimicrobial activity showed by the plant was due to the presence of these phytochemicals. Further studies are highly needed for drug development.
Corrigendum to “Incretin hormones receptor signaling plays the key role in antidiabetic potential of PTY-2 against STZ-induced pancreatitis” [Biomed. Pharmacother. 97 (330–338) 330–338] (Biomedicine & Pharmacotherapy (2018) 97 (330–338), (S0753332217332328) (10.1016/j.biopha.2017.10.071))
(Elsevier Masson SAS, 2018) Shivani Srivastava; Priya Shree; Harsh Pandey; Yamini Bhusan Tripathi
The authors regret the authors would like to change the word “Pancreatitis” to “β cells damage” throughout the manuscript, from heading to conclusion. The authors would like to change the third sentence of introduction “Thus, for diabetes progression, it is necessary to prevent the rate of pancreatitis.” to “Thus, for diabetes prevention, it is necessary to reduce the rate of β cells damage.” The authors would like to change the unit of glucose in Animal Design “200 g/dL” to 200 mg/dL.” The authors would like to change the name of secondary antibodies “anti-mouse-AF546 and anti-rabbit-AF488” to “anti-rabbit AF 546 (Red) and anti-mouse AF 488 (Green)” The authors would like to change the last sentence of discussion “All these changes collectively inhibit the apoptosis of pancreatic β cells through downregulation of Bcl 2 and improved insulin synthesis” to “All these changes collectively inhibit the apoptosis of pancreatic β cells through upregulation of Bcl 2 and improved insulin synthesis” The authors would like to apologise for any inconvenience caused. © 2017 Elsevier Masson SAS
Development and evaluation of herbal tablet loaded with Pueraria tuberosa water extract with use of different excipients
(BRNSS Publication Hub, 2018) Harsh Pandey; Shivani Srivastava; Brahmeshwer Mishra; Riden Saxena; Yamini Bhusan Tripathi
Aim: The aim of present paper is to develop the best quality of low cost tablets and with better effectiveness . Material and Methods: In this experiment we have used starch and some other excipients such as Microcrystalline cellulose, Acacia, Gellatin, Polyvinylpyrolidone and sodium Aliginate. The Water extract of plant Pueraria tuberosa belonging to the family of fabeacea. The herbal tablet of PT was made by wet granulation method. The finished products were characterized by standered method. For the determination of concentration, we have drawn the standard calibration curve of PT extract having absorption maxima at 221 nm in 0.1 N HCL solution. The drug obeyed Beer's lamberd law in the concentration range of 10 µg/ml to 50 µg/ml and was found to be linear with r2 =0.988 and regression equation y= 0. 003x- 0.009. Result and Discussion: The parameters of pre formulation such as Angle of repose, Carr's index and Husnner ratio showed good flow property. Tablets were evaluated by both official and non official testing, according to Indian Pharmacopoeia. After the official testing, the tablets made up by starch were not shown any significant change in properties . The friability of all formulations is less than 1%, except F0. Out of all, one of our formulation of herbal tablet mixed with 5% starch (FA) showed good release property within 1 hour in dissolution media, studied through in vitro method. Conclusion: These results concludes that starch can be proved as the better excipient for preparation of herbal tablets. © 2018 BRNSS Publication Hub. All rights reserved.
Engineered Cellular Uptake and Controlled Drug Delivery Using Two Dimensional Nanoparticle and Polymer for Cancer Treatment
(American Chemical Society, 2018) Sudipta Senapati; Rashmi Shukla; Yamini Bhusan Tripathi; Arun Kumar Mahanta; Dipak Rana; Pralay Maiti
Two major problems in chemotherapy, poor bioavailability of hydrophobic anticancer drug and its adverse side effects causing nausea, are taken into account by developing a sustained drug release vehicle along with enhanced bioavailability using two-dimensional layered double hydroxides (LDHs) with appropriate surface charge and its subsequent embedment in polymer matrix. A model hydrophobic anticancer drug, raloxifene hydrochloride (RH), is intercalated into a series of zinc iron LDHs with varying anion charge densities using an ion exchange technique. To achieve significant sustained delivery, drug-intercalated LDH is embedded in poly(ϵ-caprolactone) (PCL) matrix to develop intravenous administration and to improve the therapeutic index of the drug. The cause of sustained release is visualized from the strong interaction between LDH and drug, as measured through spectroscopic techniques, like X-ray photoelectron spectroscopy, infrared, UV-visible spectroscopy, and thermal measurement (depression of melting temperature and considerable reduction in heat of fusion), using differential scanning calorimeter, followed by delayed diffusion of drug from polymer matrix. Interestingly, polymer nanohybrid exhibits long-term and excellent in vitro antitumor efficacy as opposed to pure drug or drug-intercalated LDH or only drug embedded PCL (conventional drug delivery vehicle) as evident from cell viability and cell adhesion experiments prompting a model depicting greater killing efficiency (cellular uptake) of the delivery vehicle (polymer nanohybrid) controlled by its better cell adhesion as noticed through cellular uptake after tagging of fluorescence rhodamine B separately to drug and LDH. In vivo studies also confirm the sustained release of drug in the bloodstream of albino rats using polymer nanohybrid (novel drug delivery vehicle) along with a healthy liver vis-à-vis burst release using pure drug/drug-intercalated LDHs with considerable damaged liver. © 2018 American Chemical Society.
Evaluation of antimicrobial activity and bronchodialator effect of a polyherbal drug-Shrishadi
(Asian Pacific Tropical Biomedicine Press, 2012) Divya Kumari Kajaria; Mayank Gangwar; Dharmendra Kumar; Amit Kumar Sharma; Ragini Tilak; Gopal Nath; Yamini Bhusan Tripathi; J.S. Tripathi; S.K. Tiwari
Objective: To investigate antimicrobial and bronchodialator effect of hydroalcholic extract of polyherbal drug Shirishadi containing Shirisha (Albezzia lebbeck), Nagarmotha (Cyprus rotandus) & Kantakari (Solanum xanthocarpum). Methods: Antimicrobial activity was evaluated by disc diffusion method and MIC, MBC, MFC were calculated by micro dilution method. Hydroalcholic extract of this preparation was investigated for its phytochemical analysis, phenol and flavonoid were determined by spectrophotometric method and in vivo bronchodilator effect was analysed by convulsion time. Results: The phytochemical tests revealed presence of alkaloids, anthraquinones, carbohydrates, flavonoids, saponins and tannins. The antimicrobial result showed the MIC of 6.25 mg/mL against Staphylococcus aureus and 12.5 mg/mL for Escherichia coli and 12.5 mg/mL against remaining bacteria tested, with strong antifungal activity. The maximum inhibition zone is found against Pseudomonas aeruginosa with MIC 16 mg/mL. Drug showed significant bronchodilator effect with 27.86% & 36.13% increase in preconvulsion time of guinea pigs pretreated with 100 & 200 mg/kg body weight of extract. Conclusions: The study reveals that the extracts possess antibacterial activity and antifungal activity in a dose dependent manner. This antimicrobial property may be due to presence of several saponins, further studies are highly needed for the drug development. © 2012 Asian Pacific Tropical Biomedical Magazine.
Expression kinetics reveal the self-adaptive role of β cells during the progression of diabetes
(Elsevier Masson SAS, 2018) Shivani Srivastava; Harsh Pandey; Yamini Bhusan Tripathi
Objective: To determine the histopathological and molecular changes in β-cells at different time intervals following streptozotocin (STZ)-induced diabetes. Methods: STZ (65 mg/kg body weight) was given to overnight fasted rats that were sacrificed after 1, 3, and 10 days of injection. Changes in islet morphology and in the expression of various factors involved in β-cell proliferation, inflammation and apoptosis were analyzed. Results: Superoxide dismutase (Sod) expression was completely reduced and that of NF-kB and iNOS were significantly increased, along with lymphocytic infiltration in the islets within 24 h of STZ injection. In addition, the β-cell protective markers Bcl-2, IL-6, Ki67, Hif-1α, VEGF and insulin were also enhanced, indicating a compensatory response of the β-cells to the initial damaging effects. Lymphocytic infiltration decreased after 3 days of injection, accompanied by enhanced expression of both GLP-1R and GIP R. The unresponsiveness of the incretin ligands after STZ administration further suggested a compensatory approach by the incretin receptors independent of glucose regulation. After 10 days, lymphocytic infiltration and inflammatory markers again increased, along with a concomitant reduction in the expression of incretin receptors, and upregulation of the protective markers. Furthermore, the saturation peak of blood glucose indicated progressive diabetes. Conclusions: The β-cells follow a biphasic pattern of expression of certain factors in order to achieve a balance between apoptosis, autophagy, neo-genesis, hypoxia and proliferation, and achieve homeostatic protection before the onset of diabetes. The drug interventions at an early stage, which are specific to these pathways, could be beneficial in preventing the progression of diabetes pathogenesis. © 2018 Elsevier Masson SAS
GLP 1 regulated intestinal cell’s insulin expression and self-adaptation before the onset of type 2 diabetes
(Tabriz University of Medical Sciences, 2019) Shivani Srivastava; Harsh Pandey; Surya Kumar Singh; Yamini Bhusan Tripathi
Purpose: Basically insulin is known to be secreted by β cells of the pancreas. Recently, it has also been found to be produced and expressed by intestinal epithelial cells with the help of L cells secreting glucagon like peptide 1 (GLP 1). Here, we have studied the same intestinal insulin expression property in T2D rats. Methods: Following 2 weeks of high fat diet (HFD) consumption, we have been given a single dose of streptozotocin (STZ) (35 mg/kg bw). Rats were then sacrificed after 1, 7 and 21 days. The GLP 1 analogue, liraglutide was also given to one group of diabetic rats, upto their respective durations. Intestinal cells apoptosis were checked by tunnel assay, Incretin hormones secretion and dipeptidyl peptidase 4 (DPP-IV) activity were analyzed through ELISA and immunohistochemistry was used to determine the insulin expression of intestine at different time interval during diabetes progression. Results: As compared to 1 and 21 days, we have found minor cells apoptosis in 7 days group along with high level of GLP 1 in diabetic model. Further, these effects were enhanced by liraglutide. In response to these we have found, decreased insulin expression after 21 days and with no significant effect upto 7 days in diabetic control groups. In contrast to this, GLP-1 level and insulin expression enhances prominently after 7 days of liraglutide treatment. Conclusion: These results explain the self-adapting approach of intestinal cells against diabetes onset and insulin expression enhancing property of liraglutide under stressful conditions. This study should be continued in future for the development of intestinal insulin producing drugs, to control diabetes under irreversible β cells damage. © 2019 The Author (s).
Hepatoprotective role of Pueraria tuberosa water extract (PTWE) in CCl4-induced liver injury through different signaling pathways
(Springer, 2024) Prerana Aditi; Vahab Ali; Mayank Choubey; Munichandra Babu Tirumalasetty; Harsh Pandey; Shivani Srivastava; Yamini Bhusan Tripathi
Liver damage is one of the leading diseases, resulting in high morbidity. It is more relevant in the context of bad food habits, environmental pollution, and biohazards. The present study aimed to investigate the role of semi-purified water extract of Pueraria tuberosa on carbon tetrachloride (CCl4)-induced liver injury and also its mechanism of action regarding transcriptomic status in liver tissue about inflammation, hypoxia, and apoptosis. Liver injury was induced in Charles foster rats via intraperitoneal injection (IP) of CCl4, 0.1 mg/100gm body weight, diluted with olive oil (30%) twice a week for 20 days. PTWE was given via oral route daily simultaneously with CCl4 at the dose of 50 mg/100 g and 100 mg/100 g body weight. On 21st day all rats were sacrificed. Biochemical tests and histological studies were done. mRNA expression of bcl-2, caspase-3, bax, and GAPDH and protein expression of iNOS, BCL-2, HIF-1α, VEGF, β-Tubulin was done. Simultaneous treatment of PTWE with CCl4 decreased the level of NO, PC, SGOT, SGPT, ALP, LPO, and iNOS, HIF-1α, VEGF, bax, and caspase-3 expression. In addition, PTWE increased the SOD, Catalase, GSH level, and bcl-2 expression as well as normalized the architecture of hepatic tissue. Immunohistochemical staining showed the decreased accumulation of CD45, VEGF, α-SMA, collagen, and desmin after PTWE treatment. This study suggests that PTWE inhibits fibrosis by reducing the accumulation of α-SMA, collagen, and desmin in CCl4-induced toxicity. The mechanism of protective action is through its anti-inflammatory (iNOS, NO, CD45), anti-apoptotic (bcl-2, bax, caspase-3), anti-hypoxic (HIF-1α, VEGF), and anti-fibrotic (α-SMA, collagen, desmin) potentials. © The Author(s), under exclusive licence to Institute of Korean Medicine, Kyung Hee University 2024.
Hepatoprotective role of Pueraria tuberosa water extract (PTWE) in CCl4-induced liver injury through different signaling pathways
(Springer, 2025) Prerana Aditi; Vahab Ali; Mayank Choubey; Munichandra Babu Tirumalasetty; Harsh Pandey; Shivani Srivastava; Yamini Bhusan Tripathi
Liver damage is one of the leading diseases, resulting in high morbidity. It is more relevant in the context of bad food habits, environmental pollution, and biohazards. The present study aimed to investigate the role of semi-purified water extract of Pueraria tuberosa on carbon tetrachloride (CCl4)-induced liver injury and also its mechanism of action regarding transcriptomic status in liver tissue about inflammation, hypoxia, and apoptosis. Liver injury was induced in Charles foster rats via intraperitoneal injection (IP) of CCl4, 0.1 mg/100gm body weight, diluted with olive oil (30%) twice a week for 20 days. PTWE was given via oral route daily simultaneously with CCl4 at the dose of 50 mg/100 g and 100 mg/100 g body weight. On 21st day all rats were sacrificed. Biochemical tests and histological studies were done. mRNA expression of bcl-2, caspase-3, bax, and GAPDH and protein expression of iNOS, BCL-2, HIF-1α, VEGF, β-Tubulin was done. Simultaneous treatment of PTWE with CCl4 decreased the level of NO, PC, SGOT, SGPT, ALP, LPO, and iNOS, HIF-1α, VEGF, bax, and caspase-3 expression. In addition, PTWE increased the SOD, Catalase, GSH level, and bcl-2 expression as well as normalized the architecture of hepatic tissue. Immunohistochemical staining showed the decreased accumulation of CD45, VEGF, α-SMA, collagen, and desmin after PTWE treatment. This study suggests that PTWE inhibits fibrosis by reducing the accumulation of α-SMA, collagen, and desmin in CCl4-induced toxicity. The mechanism of protective action is through its anti-inflammatory (iNOS, NO, CD45), anti-apoptotic (bcl-2, bax, caspase-3), anti-hypoxic (HIF-1α, VEGF), and anti-fibrotic (α-SMA, collagen, desmin) potentials. © The Author(s), under exclusive licence to Institute of Korean Medicine, Kyung Hee University 2024.
In silico screening of Pueraria tuberosa (PTY-2) for targeting COVID-19 by countering dual targets Mpro and TMPRSS2
(Taylor and Francis Ltd., 2022) Priya Shree; Priyanka Mishra; Prateek Kumar; Harsh Pandey; Rajanish Giri; Radha Chaube; Neha Garg; Yamini Bhusan Tripathi
COVID-19 pandemic was started in Wuhan city of China in December 2019; immensely affected global population. Herein, an effort was made to identify potential inhibitors from active phytochemicals of Pueraria tuberosa (PTY-2) via molecular docking study. Our study showed five potential inhibitors (Robinin, Genistin, Daidzin, Hydroxytuberosone, Tuberostan) against Mpro and five inhibitors (Robinin, Anhydrotuberosin, Daidzin, Hydroxytuberosone, Stigmasterol) against TMPRSS2. Out of these, Robinin, Daidzin and Hydroxytuberosone were common inhibitors for Mpro and TMPRSS2. Among these, Robinin showed the highest binding affinity, therefore, tested for MD simulation runs and found stable. ADMET analysis revealed the best-docked compounds are safe and follow the Lipinski Rule of Five. Thus, it could be suggested that phytochemicals of PTY-2 could serve as potential inhibitors for COVID-19 targets. Communicated by Ramaswamy H. Sarma. © 2021 Informa UK Limited, trading as Taylor & Francis Group.
In vivo toxicity study of ethanolic extracts of evolvulus alsinoides & centella asiatica in swiss albino mice
(Open Access Macedonian Journal of Medical Sciences, 2019) Mukesh Kumar Yadav; Santosh Kumar Singh; Manish Singh; Shashank Shekhar Mishra; Anurag Kumar Singh; Jyoti Shankar Tripathi; Yamini Bhusan Tripathi
AIM: We aimed to investigate several parameters after the in vivo acute and sub-acute administration of ethanolic extracts from E. alsinoides & C. asiatica. METHODS: Malignant Ovarian Germ Cell Tumors for in vivo toxicity study guidelines 423 and 407 of Organization for Economic Co-operation and Development (OECD) were followed for acute and sub-acute toxicity assays respectively. For LD50 evaluation, a single dose of ethanolic extracts of Evolvulus alsinoides L. (EEA) and ethanolic extracts of Centella asiatica (ECA) was orally administered to mice at doses of 200, 400, 800, 1600 and 2000 mg/kg. Then the animals were observed for 72 hours. For acute toxicity evaluation, a single dose of both extracts was orally administered to mice at doses of 300, 600, 1200 and 2000 mg/kg and the animals were observed for 14 days. In the sub-acute study, the extracts were orally administered to mice for 28 days at doses of 300, 600, 1200 and 2000 mg/kg. To assess the toxicological effects, animals were closely observed on general behaviour, clinical signs of toxicity, body weight, food and water intake. At the end of the study, it was performed biochemical and hematological evaluations, as well as histopathological analysis from the following organs: brain, heart, liver, and kidney. RESULTS: The oral administration of E. alsinoides and C. asiatica ethanolic extracts, i.e. EEA 300, EEA 600, EEA 1200, EEA 2000, ECA 300, ECA 600, ECA 1200 & ECA 2000 mg/kg doses showed no moral toxicity effect in LD50, acute and sub-acute toxicity parameters. CONCLUSION: In this study, we had found that E. alsinoides & C. asiatica extract at different doses cause no mortality in acute and sub-acute toxicity study. Also, histopathology of kidney, liver, heart, and brain showed no alterations in tissues morphology. © 2019 Mukesh Kumar Yadav, Santosh Kumar Singh, Manish Singh, Shashank Shekhar Mishra, Anurag Kumar Singh, Jyoti Shankar Tripathi, Yamini Bhusan Tripathi.
Incretin hormones receptor signaling plays the key role in antidiabetic potential of PTY-2 against STZ-induced pancreatitis
(Elsevier Masson SAS, 2018) Shivani Srivastava; Priya Shree; Harsh Pandey; Yamini Bhusan Tripathi
Aims Incretin therapy is one of the most potential approaches in the treatment of diabetes. In contrast to markedly available drugs, the herbal incretin modulators have lesser side effects with low economic cost. The main aim of this work was to analyze the potential of previously reported DPPIV inhibitor, aqueous extract of Pueraria tuberosa tubers (PTY-2) as incretin hormones receptor agonist against streptozotocin (STZ)-induced diabetes. Methods Chronic diabetes was induced with STZ (65 mg/kg bw) in rats for 60 days and grouped into diabetic control and PTY-2. Expression of genes was assessed by PCR, IHC, and ELISA. Morphological analysis of tissue was observed using H & E stain. In silico molecular docking approach has been used to see the interaction of active phytochemicals of PTY-2 on the basis of their binding energy [kcal/mol] and dissociation constant [pM] using YASARA software. Interactive visualization was done using Discovery studio 3.0. Results In comparison to diabetic control, the size and number of islet cells along with the plasma level of GLP-1, GIP, and pancreatic expressions of GLP-1R, GIP-R, Bcl2, and insulin were enhanced significantly after PTY-2 treatment. Through in silico molecular docking, tuberostan showed the best interaction for GLP-1R with binding energy at 8.15 kcal/mol and dissociation constant at 1061624.125 pM. Puererone showed the best interaction for GIP-R with binding energy at 8.31 kcal/mol and dissociation constant at 810381 pM. Conclusions In addition to previously studied DPPIV inhibitor, PTY-2 also acts as incretin receptors agonist and protects against STZ-induced diabetes by down regulating β cells apoptosis. © 2017 Elsevier Masson SAS
Methanolic extract of leaves of Jasminum grandiflorum Linn modulates oxidative stress and inflammatory mediators
(2011) Adya Prasad Chaturvedi; Yamini Bhusan Tripathi
The leaves of Jasminum grandiflorum (JG) are in clinical use in Ayurveda for wound management. Since, oxidative stress and inflammation are the primary causes in delayed wound healing, so here its antioxidant and anti-inflammatory activities have been investigated using in vitro as well as in vivo models. The solvent-free methanolic extract of dried leaves of JG were tested for its trapping capacity toward pre-generated ABTS •+ radicals, instantly generated superoxide and hydroxyl radicals, along with metal chelation property, reducing power and total phenolic content. Further, it was tested on LPS-induced nitric oxide and cell viability, on primary culture of rat peritoneal macrophages. Its anti-inflammatory property was also tested on carrageenan-induced paw edema in rats. This extract significantly inhibited iron-induced lipid peroxidation and trapped ABTS •+, superoxide and OH radicals. It significantly inhibited nitric oxide (NO) release, without affecting the cell viability at 800 μg/ml concentration and reduced the formation of paw edema in rats. Thus, it could be suggested that the aforesaid anti-inflammatory properties of JG leaves are associated to its high phenolic content (2.25 ± 0.105 mg/l of gallic acid equivalent), reducing power and its free radical-scavenging property. © 2011 Springer Basel AG.
Microspheres based on mannosylated lysine-co-sodium alginate for macrophage-specific delivery of isoniazid
(2012) Sanjay Tiwari; Adya Prasad Chaturvedi; Yamini Bhusan Tripathi; Brahmeshwar Mishra
The present investigation reports coupling of ε- and α-amino groups of lysine (LS) with mannose (m-LS) and sodium alginate (SA), respectively, to reduce its toxicity. Prepared conjugate, m-LS-co-SA, was characterized through infra-red spectroscopy and differential scanning calorimetry. Cell viability studies were undertaken to assess the safety profile of the prepared conjugate. Microspheres, based on the conjugate, were prepared using spray drying technique and studied for targeting of isoniazid to alveolar macrophages (AMs). Pharmacokinetic studies of the optimized formulation batch were performed in Charles Foster rats. Infra-red spectral data of the synthesized conjugate were in agreement to the presumptive sequence of the conjugation process. Dispersibility, thermal stability and safety of the conjugate were conducive to its biomedical application. Microspheres, formulated from the conjugate, were of uniform size and offered satisfactory drug loading efficiency and in vitro release characteristics. X-ray diffraction studies established that drug was entrapped within the microspheres rather than being adsorbed on to the surface. Pharmacokinetic studies revealed that the conjugate could be a potential vehicle towards both active targeting of isoniazid to AMs and controlling its release rate. © 2011 Elsevier Ltd. All rights reserved.
Neuroprotective activity of evolvulus alsinoides & centella asiatica ethanolic extracts in scopolamine-induced amnesia in swiss albino mice
(Open Access Macedonian Journal of Medical Sciences, 2019) Mukesh Kumar Yadav; Santosh Kumar Singh; Manish Singh; Shashank Shekhar Mishra; Anurag Kumar Singh; Jyoti Shankar Tripathi; Yamini Bhusan Tripathi
AIM: To carry out the comparative nootropic, neuroprotective potentials of two medicinal plant species. MATERIAL AND METHODS: For neuroprotective activity; behavior models (elevated plus maze & morris water maze), in vivo antioxidant (superoxide dismutase, catalase, lipid peroxidation & reduced glutathione), inflammatory markers (IL-1β, IL-6 & TNF-α) and acetylcholine esterase (AChE) assessment procedures followed at different dosages i.e. 250 & 500 mg/kg of Evolvulus alsinoides and Centella asiatica ethanolic extracts. At the end of the study, it was performed histopathological analysis of the following organs: brain, heart, liver, and kidney. RESULTS: In oral administration of different doses of ethanolic extracts of both medicinal plants i.e. Sco + EEA 250 = 2.49 ± 0.29, Sco + EEA 500 = 2.67 ± 0.36, Sco + ECA 250 = 2.33 ± 0.17, Sco + ECA 500 = 2.77 ± 0.21, Sco + EEA + ECA 250 = 2.61 ± 0.32 and Sco + EEA + ECA 500 = 2.79 ± 0.16 U/mg of protein respectively against the scopolamine induced group Sco (control) = 5.51 ± 0.35 U/mg of protein extracts shows neuroprotective and nootropic activity with reducing AChE level in the brain homogenate of swiss albino mice. CONCLUSION: Since the E. alsinoides & C. asiatica are already used in traditional Indian medicine as the neuroprotective agent and also found promising effects over inflammatory diseases, wound healing, and immunomodulatory activity. The neuroprotective effect of both plants extracts attributed to inhibition of AChE activity and improve the spatial memory formation. © 2019 Mukesh Kumar Yadav, Santosh Kumar Singh, Manish Singh, Shashank Shekhar Mishra, Anurag Kumar Singh, Jyoti Shankar Tripathi, Yamini Bhusan Tripathi.