Browsing by Author "Yashvant Patel"

Now showing 1 - 6 of 6

BRN3A, a transcription factor, regulates the expression of genes involved in biological processes shaping the HPV induced cervical cancer
(Genetics Society of Korea, 2025) Anand Prakash; Yashvant Patel; Jagat Kumar Roy
Background: Cervical cancer is the fourth most common cancer worldwide in females. This occurs primarily due to the infection of high-risk Human Papilloma Virus (HPV), although in advanced stages it requires support from host cellular factors. BRN3A is one such host cellular factors, whose expression remains high in cervical cancers and upregulates tumorigenic HPV gene expression. The effect of BRN3A on HPV-mediated cervical cancer and the underlying mechanism remains obscure. Objective: To investigates the effect of BRN3A on cancer-promoting biological processes in HPV-positive uterine cervix cancer cells. Methods: We have altered the expression of BRN3A through over-expression (OE) and knock-down (KD) constructs in cervical cancer cell line, SiHa, and did transcriptome profiling through next-generation RNA-sequencing, validation through RT-PCR and BRN3A binding study with in silico promoter study and ChIP PCR methods. Results: This study revealed a substantial change in the expression of several genes associated with cancer-promoting biological processes including viral processes, immune response, cell-death, cell-proliferation, different signaling pathways, etc. Additionally, promoter analysis through in silico mode revealed that a total of 32.7% of genes possess BRN3A binding sites at their promoters. Physical interaction of BRN3A with IFITM1, OAS3, ISG15, BCL2L1 and HSP90AB1 genes was also confirmed. Conclusions: The present study identified molecular targets of BRN3A and provided new insight into the pathogenesis of cervical cancer. According to our knowledge, this is the first report on the effect on eukaryotic transcriptomes after over-expression and knocking down BRN3A. © The Author(s) under exclusive licence to The Genetics Society of Korea 2025.
Decoding the connection between lncRNA and obesity: Perspective from humans and Drosophila
(Elsevier Ltd, 2024) Dau Dayal Aggarwal; Prachi Mishra; Gaurav Yadav; Shrishti Mitra; Yashvant Patel; Manvender Singh; Ranjan Kumar Sahu; Vijendra Sharma
Background: Obesity is a burgeoning global health problem with an escalating prevalence and severe implications for public health. New evidence indicates that long non-coding RNAs (lncRNAs) may play a pivotal role in regulating adipose tissue function and energy homeostasis across various species. However, the molecular mechanisms underlying obesity remain elusive. Scope of review: This review discusses obesity and fat metabolism in general, highlighting the emerging importance of lncRNAs in modulating adipogenesis. It describes the regulatory networks, latest tools, techniques, and approaches to enhance our understanding of obesity and its lncRNA-mediated epigenetic regulation in humans and Drosophila. Major conclusions: This review analyses large datasets of human and Drosophila lncRNAs from published databases and literature with experimental evidence supporting lncRNAs role in fat metabolism. It concludes that lncRNAs play a crucial role in obesity-related metabolism. Cross-species comparisons highlight the relevance of Drosophila findings to human obesity, emphasizing their potential role in adipose tissue biology. Furthermore, it discusses how recent technological advancements and multi-omics data integration enhance our capacity to characterize lncRNAs and their function. Additionally, this review briefly touches upon innovative methodologies like experimental evolution and advanced sequencing technologies for identifying novel genes and lncRNA regulators in Drosophila, which can potentially contribute to obesity research. © 2024
Experimental evolution-induced transcriptome and phenotype responses of Drosophila melanogaster to novel thermal environments
(Company of Biologists Ltd, 2025) Dau Dayal Aggarwal; Prachi Mishra; Yashvant Patel; Manvender Singh; Vijendra Sharma; Abraham B. Korol; Pawel Michalak
Thermal stress imposes significant challenges on organisms, influencing cellular functions, morphology and survival. This study investigates the transcriptomic and phenotypic adaptations of Drosophila melanogaster populations subjected to constant high-temperature (HT) and fluctuating-temperature (FT) regimes over 80 generations in experimental evolution settings. RNA sequencing identified 1288 and 1152 differentially expressed genes in HT and FT populations, respectively, relative to the baseline population. Multiple gene ontology (GO) terms, including chromatin organization, nucleosome assembly, nucleic acid binding and polytene chromosome band formation, were enriched under both regimes, suggesting shared adaptive pathways. A weighted gene co-expression network analysis (WGCNA) revealed mitochondrial function and protein homeostasis as central to thermal adaptation, with HT populations showing enrichment of DNA repair and FT populations exhibiting enrichment of RNA processing and translation regulation-related terms. Phenotypic assays demonstrated increased heat tolerance, accelerated development and prolonged longevity in evolved populations, highlighting parallel as well as thermal regime-specific adaptive responses. This study emphasizes the complexity of transcriptomic–phenotypic adaptations to thermal stress in new environments. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Genetic Diversity and Spatiotemporal Distribution of SARS-CoV-2 Alpha Variant in India
(Multidisciplinary Digital Publishing Institute (MDPI), 2023) Jahnavi Parasar; Rudra Kumar Pandey; Yashvant Patel; Prajjval Pratap Singh; Anshika Srivastava; Rahul Kumar Mishra; Bhupendra Kumar; Niraj Rai; Vijaya Nath Mishra; Pankaj Shrivastava; P. B. Kavi Kishor; Prashanth Suravajhala; Rakesh Tamang; Ajai Kumar Pathak; Gyaneshwer Chaubey
After the spill to humans, in the evolutionary timeline of SARS-CoV-2, several positively selected variants have emerged. A phylogeographic study on these variants can reveal their spatial and temporal distribution. In December 2020, the alpha variant of the severe acute respiratory syndrome coronavirus (SARS-CoV-2), which has been designated as a variant of concern (VOC) by the WHO, was discovered in the south-eastern United Kingdom (UK). Slowly, it expanded across India, with a considerable number of cases, particularly in North India. This study focuses on determining the prevalence and expansion of the Alpha variants in various parts of India mainly by using phylospatial analysis. The genetic diversity estimation has helped us to understand various evolutionary forces that have shaped the spatial distribution of this variant during its peak. Overall, our study paves the way to understanding the evolution and expansion of a virus variant, which may help to mitigate in the case of any future wave. © 2023 by the authors.
Shatavarin-IV rescues the Di (2-ethylhexyl) phthalate (DEHP) induced oxidative stress in rat granulosa cells in vitro
(Elsevier Inc., 2024) Vivek Pandey; Alka Sharma; Sonal Tiwari; Yashvant Patel; Jayhind Kumar Chauhan; Safiya Ayesha; Alakh N. Sahu; Rashmi Gupta; Anima Tripathi; Pawan K. Dubey
Studies provide notable evidence that oxidative stress (OS) mediated reactive oxygen species (ROS) disturb reproductive health. We have shown in our previous publication that exposure of Di-(2-ethylhexyl) phthalate (DEHP), induces OS mediated ROS generation which inhibits steroid synthesis. In the present study, we demonstrated the ameliorative/protective effects of one of the steroidal saponins, i.e., Shatavarin-IV, isolated from the roots of Asparagus racemosus against DEHP induced OS in rat granulosa cells. Granulosa cells were exposed with DEHP alone (400 μM), Shatavarin-IV alone (8 μg/ml), and a combination of DEHP + Shatavarin-IV (400 μM + 8 μg/ml) in vitro for 24 hrs. Intracellular ROS, OS/hypoxia, mitochondrial membrane potential, steroid-responsive genes expression were analyzed. The results revealed that the effective dose of DEHP (400 µg) significantly increased OS compared to the control by increasing ROS levels, mitochondrial membrane potential, and β-galactosidase activity with a higher level of apoptotic genes (Bax, Caspase-3) expression at mRNA level. Further, DEHP significantly (p < 0.05) reduced mRNA expression of steroidogenic responsive genes (StAR, CYP17A1, and CYP19A1) in granulosa cells treated with above combination compared to control. Interestingly, co-treatment of DEHP + Shatavarin-IV significantly suppressed the DEHP induced OS, ROS, β-galactosidase levels and enhanced steroidogeneic and apoptotic gene expression activities, which suggests that Shatavarin-IV rescued DEHP-induced changes that may useful for the prevention of DEHP- induced reproductive toxicity. © 2024 Elsevier Inc.
THPM (1,2,3,4-Tetrahydro pyrimidine) and Berberine Exhibit Synergistic Impact on Inhibition of Cell Migration and Colonization Through ROS-Mediated Apoptotic Pathways in the Breast Cancer Cells
(John Wiley and Sons Inc, 2025) Rakesh Kumar Gupta; Ambarish Priyadarshan; Sonal Tiwari; Yashvant Patel; Arun K. Bind; Garima Tripathi; Anima Tripathi; Abhijeet Kumar; Pawan Kumar Dubey
Breast cancer is one of the major causes of death in females worldwide. Considering the polypharmacological trend, small molecules like pyrimidine along with berberine, a bioactive anticancer agent may act as effective anticancer drugs having multiple targets with minimal side effects. However, it has never been tested. The present work discloses the efficacy of the combination of 1,2,3,4,-Tetrahydro pyrimidine (THPM) and Berberine (BBR) in inhibiting cancer progression in human breast cancer cell line, i.e., MCF-7. THPM were synthesized and characterized, and their anti-cancerous potential was evaluated by in silico study. The MCF-7 cells were exposed in vitro with THPM (200 µM), BBR (20 µM/ml) alone or in combination of THPM + BBR (200 µm + 20 µm/ml) for 24 h. Intracellular ROS, annexin-V staining, cell migration, colony formation assay, and gene expression analysis were performed. THPM and BBR both exhibited solid binding affinity against the BCL-2 protein. Interestingly, co-treatment of THPM + BBR significantly increased ROS level, annexin-V positive cells, the expression level of Bax, and Caspase-3 and inhibited migration and proliferation of MCF-7 cells. In conclusion, co-treatment of THPM + BBR exhibits a synergistic impact via inhibiting cell proliferation and inducing ROS-mediated apoptosis in MCF-7 cells suggesting that THPM and BBR could be used as novel therapeutic agents against breast cancer. © 2025 Wiley-VCH GmbH.