Temperature dependent polymorphism of pyrazinamide: An in situ Raman and DFT study

Abstract

The α and γ polymorphs of drug pyrazinamide have been detected with the help of temperature dependent Raman spectroscopic technique. Pyrazinamide is a very useful drug used for the treatment of tuberculosis (TB) and plays a significant role in destroying the dormant tubercle bacilli which are not destroyed by other common TB drugs. Temperature dependent Raman spectra suggest polymorphic phase change from α → γ form of pyrazinamide between 145 and 146 °C. In situ Raman spectra of pyrazinamide between 145 and 146 °C show the conversion of α → γ form by the shift in C[dbnd]O stretching vibration accompanied by several other changes. The phase change is characterized by the breaking of two linear N[sbnd]H ⋯ O type hydrogen bonds associated with C[dbnd]O stretching vibration in α dimer and formation of one linear N[sbnd]H ⋯ N type hydrogen bond along with a weak intramolecular C[sbnd]H ⋯ O type hydrogen bond in the γ dimer. © 2017