Monitoring the genetic fidelity of micropropagated plantlets of Spondias mangifera Willd. using RAPD marker assays

Abstract

Random amplified polymorphic DNA (RAPD) markers were used to screen for clonal fidelity in in vitro-propagated plantlets of Spondias mangifera produced through direct organogenesis. One micropropagated plantlet was selected at random after each sub-cultural passage (six sub-cultures), along with the donor plant, for RAPD marker analysis. Twenty-five RAPD primers were used to study genetic similarities or dissimilarities with the mother plant as well as among the regenerated plants. Individual primers showed that the same pattern of RAPD markers was shared by all in vitro-propagated plantlets and the mother plant. No variation was observed among the micropropagated progenies. Thus, in vitro-regenerated plantlets of S. mangifera were clonally uniform and genetically stable.