Purification, characterization and assessment of stability, reactive oxygen species scavenging and antioxidative potentials of mycosporine-like amino acids (MAAs) isolated from cyanobacteria

Abstract

Mycosporine-like amino acids (MAAs) were characterized and their stability and free radical scavenging potentials were investigated in Anabaena sp. HKAR-7 and Fischerella sp. AR-5. UV/VIS absorption spectroscopy, high performance liquid chromatography and electrospray ionization-mass spectrometry showed occurrence of diverse forms of MAAs at retention time (RT) 1.16 (shinorine), 2.18 (mycosporine glycine-310) and 3.14 min (palythinol) with UVλmax 310, 332 and 334 nm respectively, in Fischerella sp. when contrasted with Anabaena sp. (prominent peak at RT 3.21 min (porphyra 334; P-334) with UVλmax 334 nm. MAAs showed dose-dependent in vitro antioxidative and in vivo reactive oxygen species (ROS) scavenging potentials. The MAA P-334 was used against strong allelochemical pyrogallic acid in Anabaena sp. P-334 reducing the negative impacts brought about by ROS, in this way, the malondialdehyde content and unwinding of dsDNA were similarly low. This clarifies the role of MAA P-334 against cell’s ROS under studied stressed conditions. © 2022, The Author(s), under exclusive licence to Springer Nature B.V.