Identification of elite species of Ophiorrhiza utilizing HPTLC analysis and camptothecin as a phytochemical marker: Assessment of extraction effectiveness and organ selection

Abstract

Objective: The main objective of this experiment is to find the camptothecin (CPT) content in different parts of three Ophiorrhiza spp. Methods: In this study, three species of Ophiorrhiza viz., O.mungos L., O. hirsutula Wight ex Hook. f. and O.rugosa var prostrata (D.Don) Deb & Mondal were taken. Different parts of plant sample such as inflorescence, leaf, stem, and roots were extracted in methanol (100mg/10 ml w/v) using microwave and screened for camptothecin content by High-performance thin layer chromatography (HPTLC). After the selection of elite species different extraction techniques such as Maceration, Hot water bath extraction, Microwave assisted extraction (MAE), and Ultrasonic assisted extraction (UAE) were performed on the elite species for optimization of extraction technique. Results: High performance thin layer chromatography confirmed the presence of camptothecin in all three species. Highest was observed in O.mungos(0.37%) followed by O.hirsutula(0.11%) and a negligible amount in O.rugosa var prostrata(0.001%). Ultrasonic assisted extraction (UAE) method showed the best results in O.mungos leaves. © 2023 Elsevier Ltd