Preliminary analysis of cuprome of Anabaena doliolum using two-dimensional gel electrophoresis

Abstract

This study provides first-hand information on the initial characterization of copper-induced changes in the global proteome (hereafter called cuprome) of Anabaena doliolum subjected to short- and long-term treatments. PD Quest analysis revealed that out of 215 protein spots in the control, 79 showed alterations (26 up- and 36 down-regulation, and 5 up- and 12 down-regulation respectively, after 24 and 168 h of Cu treatment) in their expression pattern. The short-term (24 h) and long-term (168 h) treatments induced respectively, 158 and 96 proteins. Of the 158 newly induced proteins, 30 were found to sustain the long-term treatment. In view of the appearance of two sets of proteins, there appears a need to carry out short- and long-term proteome analysis for getting a holistic view of the proteome of cyanobacteria subjected to abiotic stress.