Alloxan-induced diabetes in rabbits and effect of a herbal formulation D-400

Abstract

Adult male rabbits injected with alloxan (50 mg/kg, i.p.) were divided into two groups of nine each. One group received placebo and the other group an aqueous suspension of D-400, at the dose of 1 gm/kg body weight orally daily for 36 weeks. Blood glucose, blood urea and serum creatinine were estimated initially and at every 6-weekly intervals. At the end of 36 weeks D-400 significantly prevented the rise in blood urea and serum creatinine levels as compared to the control. Although a rise in blood sugar was noticed in both the groups, the level of blood sugar after 36 weeks was significantly lower in the D-400 treated group. This shows the favourable response of D-400 against alloxan-induced renal damage and hyperglycaemia.