Folic acid, total antioxidant and malondialdehyde in fetal cord blood of preeclamptic pregnancy

Abstract

The present study was' designed to evaluate the folic acid, total antioxidant and malondialdehyde (MDA) status in fetuses of Pre-eclamptic pregnancy.52 cases were studied of which 26 were from normal pregnancy (controls) and 26 were from Preeclamptic pregnancy. Fetal cord blood (10 mil) was collected from the umbilical vein in heparinised tubes. It was centrifuged for ten minutes and the plasma collected. Folic acid was estimated by ELISA method, total antioxidant (TAS) was determined by Rondox kit and level of Malondialdehyde (MDA) was estimated by thiobarbituric acid (TBARS) assay from the collected plasma. In pre-eclampsia, the folic acid and total antioxidant was significantly decreased as compared to those in normal subjects, whereas Malondialdehyde was significantly increased. A significant negative correlation was detected between folic acid, total antioxidant and lipid peroxidation in pre-eclampsia. Our study clearly indicates a relationship between decreased folic acid, total antioxidant and elevated lipid peroxidation in pregnancy with preeclampsia.