Mutagenic effects of gamma rays and EMS IN M1 and M2 generations in two traditional genotypes of aromatic rice (oryza Sativa L.)

Abstract

With the aim to know immediate effect of mutagenic treatment in two traditional non Basmati rice varieties viz. Kalanamak and Badshah Bhog, in terms of biological damage. We examined reduction in seeds germination, root and shoot length, plant survival at maturity, pollen fertility and grain yield in M1 generation. Most of the mutagenic treatments severely affected the genotypes, Kalanamak and Badshah Bhog both. A dose dependent increased in biological damage was observed in most of the mutagenic treatment and maximum biological damaged was caused by 40 kR gamma rays + EMS (0.2%) followed by 30kR gamma rays+EMS(0.2%) treatment, while minimum at 10kR gamma rays. Biological damage was relatively more in Badshah Bhog as compared to genotype Kalanamak. A linear relationship was observed between doses of gamma ray and damage in different biological parameters, such as, plant survival at maturity, root and shoot growth, pollen fertility as well as grain yield. The mutagenic efficiency was found maximum at 0.2% EMS in Kalanamak and Badshah Bhog and the mutagenic effectiveness was found maximum at 10 kR gamma rays in both the varieties Kalanamak and Badshah Bhog.