Effect of cisplatin, lipopolysaccharide, muramyl dipeptide and recombinant interferon-gamma on murine macrophages in vitro. II. Production of H2O2, O2̄ and interleukin-1

Abstract

Murine macrophage monolayers treated with cisplatin, lipopolysaccharide (LPS), muramyl dipeptide (MDP) or recombinant interferon-γ (rIFNγ) for 2-48 h showed significant increases in the release of H2O2, O2̄ and interleukin-1 (IL-1) as compared to unrelated macrophages. The treatment of macrophages with different combinations of the above agents did not induce synergistic or additive effects on the production of H2O2, O2̄ and IL-1. The priming of macrophages with rIFN-γ had a significant effect in the additional increased production of H2Ō, O2- and IL-1 when subsequently treated with cisplatin, LPS or MDP.