DNA barcoding and genetic diversity studies of Water lily aphid, Rhopalosiphum nymphaeae from India

Abstract

Aim: In this study, DNA barcoding of Rhophalosiphum nymphaeae was conducted using a population collected from five different aquatic host plants, and the genetic diversity analysis was performed. Methodology: Genomic DNA from R. nymphaeae adults was extracted and the COI gene was amplified using PCR. The PCR products were sequenced, analyzed for identification using NCBI and BOLD databases, and the sequences were submitted to NCBI GenBank. Further genetic analysis were conducted to study the genetic diversity of the pest. Results: Two new aquatic weed hosts of water lily aphid was reported from India, i.e., Blue water lily, Nymphaea nouchali Burm. f., and Four leaf clover, Marsilea quardrifolia L. The genetic diversity analysis revealed the lesser diversity among the Indian and USA population of water lily aphid as compared to the populations from China, Canada and Lithuania. Interpretation: The genetic diversity analysis indicated no genetic variation in the Indian population, however, a moderate level of variation was observed in the rest of the world population. This work will facilitate the quick identification of R. nymphaeae independent of sex and stage, furthermore helping in either pest management or its use as bio-control agent. © 2025 Triveni Enterprises. All rights reserved.