A validated rp-hplc method for the estimation of diosgenin from polyherbal formulation containing Tribulus terrestris Linn

Abstract

In present study a simple, rapid and specific high-performance-liquid-chromatographic (HPLC) method has been developed and validated for estimation of bioactive marker diosgenin (DSG) from polyherbal formulation containing gokhru (Tribulus terrestris Linn.) extract. Separation of DSG was achieved on reverse-phase 250mm×4.6mm, 5μ, symmetry-C8 column with mobile phase of water and methanol. Scanning wavelength was optimized and selected as 205nm. The linear regression analysis of calibration plots showed good linear relationship with r2=0.9996 in concentration ranges of 25-75μg/mL. The method was validated for its calibration curve, specificity, precision and robustness. The recovery was found in range of 98.06-99.50%. The developed HPLC method can be used to quantify DSG for quality control and standardization of polyherbal formulations containing gokhru. The analytical data were compared by statistical evaluation and the method was found to be repeatable and selective.