In-silico study of transcription factor binding elements of human PAX gene family members

Abstract

PAX gene family members, tissue specific transcription factors mainly involved, in the formation of tissues and. organs during embryonic development and has important role in transcriptional regulation. The presence of consensus paired domain play significant role in DNA-binding transcription regulation with PAX domain. Regulatory behavior of PAX family members were determined using cis-acting elements study and repeat identification. The study helped in investigating the potential conserved motifs in the paired domain. Further, investigation of cis-acting elements was done to elucidate the function for each PAX members and then repeat analyses and their correlation with functional elements were done. The study illustrates that the cis-acting elements are involved in tissue specific developmental expression and transcription al regulation of PAX family members. Further, based on physiochemical property study of these PAX gene family members it was found that they are mainly Ser, Pro, Gly and Ala rich amino acids. It was found that repeats containing functional DNA motifs interact with signature motifs of paired domain. The mam six signature motifs NQLGG, NGRPLP, RPC, SR, GCVSKIL and PGAIGGSKP are involved in interaction. Altogether, this study provides new insights into the regulatory behavior of upstream region of each PAX members and its effect in trans criptional regulation and developmental expression of these PAX members with involvement in disease management. © 2013 Asian Network for Scientific Information.