Uricase conjugated nanostructured NiFe2O4/rGO modified flexible screen-printed carbon electrode based interface for long-range electrochemical determination of uric acid

Abstract

Uric acid (UA) is a biomarker linked with gout and kidney stones that is harmful to the human body. Thus, this study explores the detection of UA using the differential pulse voltammetry (DPV) method. Initially synthesizing a highly active Nickel ferrite/reduced graphene oxide (NiFe2O4/rGO) nanocomposite ferrite material by simple surfactant-free low-temperature hydrothermal approach. The as-synthesized material was studied using various spectroscopic and microscopic techniques. The bioelectrode was fabricated on a screen-printed carbon electrode (SPCE), and the as-prepared Uricase/NiFe2O4/rGO/SPCE demonstrated improved electrochemical oxidation peak current (Ipa). While its electrochemical biosensing performance towards various UA concentrations shows a wide linear range from 5 to 900 μM, with a lower limit of detection (21·9 μM), high sensitivity (1·05×10−4 mA/μM cm2), good selectivity (RSD < 5·7 %), excellent stability, and repeatability. In addition, the fabricated bioelectrode was also explored to quantify the amount of UA in bovine serum albumin (BSA) real spiked samples. Hence, the proposed biosensor has potential applications in point-of-care-treatment of numerous disorders related to UA metabolism. © 2024