Title:
MALDI mass sequencing and characterization of filarialglutathione-S-transferase

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dc.contributor.authorSarika Gupta
dc.contributor.authorAnchal Singh
dc.contributor.authorMarshleen Yadav
dc.contributor.authorKalyan Singh
dc.contributor.authorSushma Rathaur
dc.date.accessioned2026-02-07T04:47:09Z
dc.date.issued2007
dc.description.abstractGlutathione-S-transferase has been detected in the somatic extract and excretory-secretory products of different life stages of Setaria cervi, a bovine filarial parasite. The enzyme was subjected to MALDI-TOF followed by mass spectrometry and the nearest match found was Pleuronectes platessa GST. Molecular mass of the purified enzyme was≈26 kDa as determined by SDS-PAGE and MALDI-TOF. Setaria cervi GST exhibited high activity towards 1-chloro-2,4-dinitrobenzene and ethacrynic acid. Kinetic analysis with respect to 1-chloro-2,4-dinitrobenzene and glutathione as substrate revealed a Km of 2.22 mM and 0.61 mM, respectively. The activity was inhibited significantly by Cibacron blue and α-tocopherol. © 2007 Elsevier Inc. All rights reserved.
dc.identifier.doi10.1016/j.bbrc.2007.02.147
dc.identifier.issn10902104
dc.identifier.urihttps://doi.org/10.1016/j.bbrc.2007.02.147
dc.identifier.urihttps://dl.bhu.ac.in/bhuir/handle/123456789/19408
dc.subjectFilariasis
dc.subjectGlutathione-S-transferase
dc.subjectMALDI mass
dc.subjectSetaria cervi
dc.titleMALDI mass sequencing and characterization of filarialglutathione-S-transferase
dc.typePublication
dspace.entity.typeArticle

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