Cloning and expression analysis of nagJ hyaluronidase gene of Clostridium chauvoei

Abstract

Black quarter caused by Clostridium chauvoei, a Gram positive anaerobe, is an important bacterial disease of ruminants. Though role of virulence factors such as cctA, sialidase and flagellin is well documented, hyaluronidases of C. chauvoei are not yet characterized. So, the present study was aimed at cloning and expression of hyaluronidase (nagJ) gene of C. chauvoei. The nagJ gene of C. chauvoei was amplified by PCR and cloned and expressed in prokaryotic expression system. Further, expressed recombinant hyaluronidase protein was purified and the expression was confirmed by SDS-PAGE and Western blotting analysis. Further investigation is needed to evaluate its functional activity and role in virulence of C. chauvoei. © 2018 Indian Council of Agricultural Research. All Rights Reserved.