Nested multiplex PCR detection of human papillomavirus 16 and 18 in pre-invasive lesions and its implication in screening of carcinoma cervix

Abstract

Introduction: Carcinoma cervix is a preventable disease and is caused by certain high risk Papillomaviruses. In the present study, our aim was to investigate the utility of nested multiplex Polymerase Chain Reaction in detecting HPV 16 and 18 in cervical scrapes/biopsy samples and to correlate with cervical cytology/ histopathology findngs. Methods: A total of 119 females were subjected for Papanicolaou smear examination of cervical scrapes and/ or histopathological examination of cervical tissues. These samples were also subjected to nested multiplex Polymerase Chain Reaction targeting HPV 16/ 18 specific E6/7 gene sequences. Results: HPV 16/18 were detected in 33.6% (40/119) cases included in the study. The overall HPV 16/ 18 positivity among cases with Negative for Intraepithelial Lesion or Malignancy, Low grade Squamous Intraepithelial Lesion, and High grade Squamous Intraepithelial Lesion was observed to be 20.8%, 44%, and 66.7% respectively. Positivity for HPV 16 in cases with SCC was found to be 80%. HPV positivity among subjects reported with reactive cellular changes, a sub category of Negative for Intraepithelial Lesion or Malignancy, was observed to be 26.6%. Conclusion: HPV 16 and 18 positivity in cases reported with different stages of pre invasive lesions of carcinoma cervix, particularly in the subcategory reactive cellular changes of Negative for Intraepithelial Lesion or Malignancy, indicates that NMPCR detection of HPV 16/ 18 may be used as a screening tool for carcinoma cervix in conjunction with Papanicolaou smear examination.