Immunochemical detection of serum LDH1: An indigenous method in the diagnosis of myocardial infarction

Abstract

Immunochemical studies on lactate dehydrogenase (LDH) were carried out to develop an indigenous immunochemical method for the diagnosis of acute myocardial infarction (AMI). Heart-specific LDH1 was purified and polyclonal antibody against LDH1 was raised in rabbits. Immunodiffusion and immunoblotting identifications show the presence of LDH1 in AMI test samples. Immunochemical detection of LDH1 in human serum may substitute kinetic method of total LDH assay and electrophoretic analysis of LDH1 isozyme in the diagnosis of AMI.