Shoot regeneration from immature cotyledons of Cicer arietinum

Abstract

Shoot regeneration was achieved from immature cotyledons of five chickpea (Cicer arietinum L.) genotypes: C235, ICC4971, ICC11531, ICC12257 and ICC12873. The cotyledons cultured on Murashige and Skoog (MS) medium supplemented with 3 or 5 mg dm-3 zeatin with or without 0.04 mg dm-3 indole acetic acid (IAA) showed formation of cotyledon like structures (CLS) at their proximal ends. Subsequently, shoot regeneration took place in some of the CLS forming explants. CLS were also formed in cotyledons cultured on MS + 0.2 - 1 mg dm-3 thidiazuron (TDZ); direct shoot regeneration was observed in cotyledons cultured on 1 mg dm-3 TDZ. The shoot buds elongated on media containing indole butyric acid (IBA), benzylaminopurine (BAP) and gibberellic acid (GA3). Complete plantlets were obtained by rooting of shoots following pulse treatment with 200 mg dm-3 IBA for 5 min and culture on growth regulator free half-strength MS medium.