In Vitro Propagation of Guava from Shoot Cultures of Mature Trees

Abstract

Studies on the in vitro propagation of Psidium guajava L. demonstrate that shoot tip explants from mature trees are capable of forming multiple shoots. Proliferation of shoot tip was obtained on MS basal medium containing different combinations and concentrations of auxins and cytokinins. However, highest number of shoots per explant was obtained from cultures grown on medium supplemented with 1 mg l-1 BAP only. Rooting was achieved by transferring the individual shoots to 1/2 strength MS medium having 0.1-0.5 mg 1-1 IBA and NAA. About 80% shoots rooted well when medium was supplemented with IBA and NAA (0.2 mg 1-1 each) together. Addition of activated charcoal showed beneficial effects on rooting percentages and plantlet growth. Plantlets were acclimated and successfully established in soil. © 1987, Gustav Fischer Verlag, Stuttgart. All rights reserved.