Synthesis, characterization, and in vitro drug release study of 3-arm poly-β-alanine

Abstract

Synthesis of three arms star-shaped poly-β-alanine (3-b-ala) based on tri(prop-2-yn-1-yl) benzene-1,3,5-tricarboxylate (TBT) and azido terminated poly-β-alanine (N3-P-ala) was performed using click reaction. TBT was synthesized by nucleophilic substitution reaction between propargyl alcohol and 1,3,5-benzenetricarbonyltrichloride. For the first time, N3-P-ala was synthesized through anionic polymerization of acrylamide using sodium azide as an initiator. TBT was characterized by FT-IR and 1HNMR. N3-p-ala was characterized by FT-IR, GPC, and 1HNMR and 3-b-ala was characterized by FT-IR, GPC, 1HNMR, TGA, and XRD. The synthesized 3-b-ala was used for drug loading and releasing studies. Polymer loaded drug (3-b-ala-D) hybrid was used in in vitro studies of drug (Diclofenac sodium) release in phosphate buffer solution (PBS) at 37 ± 0.5°C and pH 7.4. The drug loading and releasing studies were analyzed by UV-visible spectrophotometer. 3-b-ala-D was examined by AFM to analyze the surface morphology and roughness. © 2015 Wiley Periodicals, Inc.