Plant tissue culture in tree species

Abstract

Growing cells, tissues, and organs on nutrient media under aseptic and controlled condition is called plant tissue culture. It has several applications such as mass multiplication of plants, germplasm conservation, production of pathogen-free plants, secondary metabolite production, and transgenic plant production. It is highly useful in understanding basic and applied aspects of plant science such as embryology, morphogenesis, cytology, pathology, physiology, biochemistry, etc. For improvement of plants through genetic manipulation, an efficient plant regeneration protocol is essential requirement. Improvement of several crops has become possible by screening and selecting superior somaclonal and gametoclonal variants. Commercial production of such plants has several benefits over conventional breeding techniques, as it takes less time with less labor input. © 2022 Elsevier Inc. All rights reserved.