Advancements in estimating post-mortem interval in medico-legal practice: A comprehensive review

Abstract

Estimating the Post-Mortem Interval (PMI) is a crucial aspect of forensic investigations, aiding in the resolution of criminal cases, identifying missing persons, and understanding decomposition processes. This review provides an exhaustive analysis of recent advancements in PMI estimation techniques, encompassing both traditional and emerging methodologies. Included in the study is an exhaustive examination of well-established methodologies, including algor mortis, livor mortis, and rigor mortis, as well as their shortcomings and improvements. It also delves into innovative approaches, including forensic entomology, chemical and molecular biology, microbiology, artificial intelligence, etc. Furthermore, this article discusses the integration of multiple disciplines and the potential of interdisciplinary collaboration to enhance PMI accuracy and reliability. By synthesizing the latest research findings and technological innovations, this review aims to provide forensic practitioners, law enforcement agencies, and medico-legal professionals with a comprehensive understanding of the current state-of-the-art in PMI estimation, facilitating more effective crime scene investigations and judicial proceedings. © 2025 Elsevier B.V.