Krishna, A.Abhilasha, S.2025-02-212025-02-21200084301https://dl.bhu.ac.in/ir/handle/123456789/52669Follicular kinetics were studied using immunocytochemical localization of proliferating cell nuclear antigen (PCNA) and changes in circulating steroid concentrations to investigate the cause of prolonged survival of Graafian follicles and delayed ovulation in the vespertilionid bat Scotophilus heathi. PCNA immunoreactivity was limited to the granulosa cells and varied with stage of follicular development and reproductive phase. Two waves of follicular growth appear to be initiated in October and February. The first wave resulted in the appearance of some late antral follicles in November. These antral follicles grew slowly and differed from typical mammalian Graafian follicles, as the cells of their cumulus oophoricus were large and hypertrophied. The second wave of follicular development resulted in fresh recruitment and selection of dominant follicles in February. The steroid-hormone profile showed an unusually high concentration of circulating androstenedione from October to December. The estradiol concentration showed two peaks coinciding with two peaks of PCNA immunoreactivity. The results show that the unusually high androstenedione concentration might be responsible for the development of non-ovulatory Graafian follicles with hypertrophied granulosa cells, which survive for a prolonged period. The decline in androstenedione concentration allows the development of ovulatory follicles in S. heathi.Articlehttps://doi.org/10.1139/z00-053