Dwivedi, RomiSingh, SaumyaChauhan, Brijesh SinghSrikrishna, S.Panday, Anoop KumarChoudhury, Lokman H.Singh, Vinod Prasad2025-03-012025-03-01202010106030https://dl.bhu.ac.in/ir/handle/123456789/67727An aroyl hydrazone based fluorescent probe, hpsh, has been developed for the selective detection of Cu2+ ions in pure aqueous medium by static fluorescence quenching. The fluorescence quenching of hpsh in the presence of Cu2+ takes place as a result of ground state complex formation through intramolecular charge transfer (ICT). Addition of Cu2+ ions changes the color of the solution from colorless to yellow-green which is clearly visible by naked eye. Large Stokes shift of hpsh prevents the self-quenching of the probe in absence of metal ions. The observed stoichiometry between Cu2+ and probe has been found as 1:2 (M: L). MTT assay of hpsh on fruit flies confirms that the probe is non-toxic and biocompatible. The plausible in vivo bioimaging application of the probe to detect Cu2+ in Drosophila gut tissues as well as in adult fruit fly has been investigated with excellent results. © 2020 Elsevier B.V.Bio imagingFluorescent probeLarge stokes shiftStatic quenchingArticlehttps://doi.org/10.1016/j.jphotochem.2020.112501