Catechin isolated from faba beans (Vicia faba L.): insights from oxidative stress and hypoglycemic effect in yeast cells through confocal microscopy, flow cytometry, and in silico strategy

Choudhary, Dhiraj KumarChaturvedi, NavaneetSingh, AmitMishra, Abha2025-01-272025-01-2720227391102https://dl.bhu.ac.in/ir/handle/123456789/15266The present aim of this investigation was to evaluate the effect of catechin from faba beans on oxidative stress and glucose uptake in yeast cells. Flow cytometry approach indicated that 2-NBDG (1.98 � 0.37) seed extract had a lower relative fluorescence signal than methanol (5.98 � 0.67) and acetone seed extract (4.43 � 0.55). In comparison to the control and seed extract, H2O2 exposure increased the apoptosis rate of yeast cells from 8.20% to 64.80%. Yeast cells incubated with H2O2 produced significantly more ROS intensity (162 � 4.32, p < 0.05) than control cells (118 � 2.52, p < 0.05) and less than seed extract-treated cells. Molecular dynamics simulation studies were performed for cat:?-amylase (catechin-?-amylase complex) which revealed the stable and mixed mode of inhibition during a simulation. The synergistic action of polyphenols or catechin present in seed extract may be responsible for the anti-oxidative stress and hypoglycaemic effects. The findings of this study may provide insight into the further development of a novel antidiabetic drug for T2DM. Communicated by Ramaswamy H. Sarma. � 2021 Informa UK Limited, trading as Taylor & Francis Group.antidiabetic drugfaba beanFlow cytometrymolecular dynamics simulationpolyphenolssynergistic effectCatechin isolated from faba beans (Vicia faba L.): insights from oxidative stress and hypoglycemic effect in yeast cells through confocal microscopy, flow cytometry, and in silico strategyArticlehttps://doi.org/10.1080/07391102.2021.1945953