Kujur, AnupamKumar, AkshayPrakash, Bhanu2025-01-302025-01-302021483575https://dl.bhu.ac.in/ir/handle/123456789/25482The present study investigated the novel antifungal, and anti-aflatoxin B1 mechanism of Eugenia caryophyllata L. essential oil (ECEO) loaded chitosan nanomatrix against the toxigenic strain of A. flavus (AFLV-DK-02). Phytochemical profiling of ECEO was done by GC�MS which revealed eugenol (73.6%) as the primary bioactive compound. ECEO was encapsulated inside the chitosan nanomatrix (ECEO-Np) and characterized using SEM, AFM, FTIR and XRD analysis. The ECEO-Np exhibited enhance antifungal (0.25 ?L/mL) and anti-aflatoxin B1 inhibitory activity (0.15 ?L/mL) than ECEO. Antifungal and antiaflatoxin B1 inhibitory activity was found to be related with impairment in the biological functioning of the plasma membrane (ergosterol synthesis, leakage of membrane ions, UV light (260, 280 nm) absorbing material, dead cell by propidium iodide assay, mitochondrial membrane potential (MMP), methylglyoxal and inhibition in essential carbon substrate utilization). ECEO-Np exhibited remarkable free radical scavenging activity with IC50 value of 0.002 ?L/mL. ECEO-Np effectively preserves the sensory characteristics of exposed maize crop seed up to six months of storage and shows considerable safety profile (non-toxic, non-mutagenic, non-hepatotoxic, non-carcinogenic, non-tumorigenic and biodegradable) using computational ADMET (absorption, distribution, metabolism, excretion, and toxicity) analysis. � 2020 Elsevier Inc.AntifungalChitosan nanomatrixEugenia oilMode of actionSafety profileArticlehttps://doi.org/10.1016/j.pestbp.2020.104755