Tiwari, ShikhaUpadhyay, NehaSingh, Bijendra KumarDubey, Nawal K.Dwivedy, Abhishek K.Singh, Vipin Kumar2025-01-272025-01-2720229505423https://dl.bhu.ac.in/ir/handle/123456789/13964The study explores in�vitro antifungal and aflatoxin B1 inhibitory potency of chemically characterised Lippia origanoides EO (LOEO) encompassed in chitosan nanoparticle (CS-LOEO-Np). CS-LOEO-Np was physico-chemically characterised through XRD, SEM and FTIR analyses. CS-LOEO-Np exhibited improved antifungal and AFB1 inhibitory efficacy (0.05 and 0.045 �l mL?1, respectively) in contrast to LOEO (0.30 and 0.25 �l mL?1, respectively). Bioactivity of LOEO loaded nanoparticle was enhanced as reflected by depletion in ergosterol content, rapid cellular ion release and reduced methylglyoxal content. IC50 value equivalent to 4.17 �l mL?1 displayed better antioxidant potency of CS-LOEO-Np as compared to LOEO (IC50 = 6.20 �l mL?1). CS-LOEO-Np preserved sensorial attributes of stored Nigella sativa (model food matrix) samples and have higher lethal toxicity dose, that is LD50 = 8832 mg kg?1. Hence, CS-LOEO-Np could serve as novel green candidate to ensure food security with better nutritional and sensorial features. � 2022 Institute of Food Science and TechnologyAflatoxin B₁antioxidantchitosanLippia origanoidesmethylglyoxalArticlehttps://doi.org/10.1111/ijfs.15639